An effective screening for proliferative vitreoretinopathy-associated genes

• Project/Area Number: 24592687
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Fukuoka University
• Principal Investigator: SASSA, Yukio 福岡大学, 医学部, 講師 (80580315)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Shigeo 九州大学, 医学部, 講師 (50363370) ; ISHIBASHI Tatsuro 九州大学, 医学部, 教授 (30150428) ; FUKUSHIMA Syuuichiro 大阪大学, 基礎工学部, 助教 (40362644) ; YASUI Takeshi 徳島大学, 工学部, 教授 (70314408) ; KONO Tosihiro 福岡大学, 医学部, 教授 (40117106)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 眼増殖性疾患 / 網膜色素上皮細胞 / SHG光 / コラーゲン / リモデリング / SHG顕微鏡 / ペリオスチン / テナシンC / 牽引性網膜剥離 / 増殖糖尿病網膜症 / 増殖性網膜症 / コラーゲン可視化 / テナシンＣ / コラーゲンリモデリング / レーザー脈絡膜新生血管モデル / 走査電子顕微鏡 / 線維化

Outline of Final Research Achievements

We recently have revealed the ocular proliferation-associated genes by microarray analysis of intraocular proliferative tissues. We have previously reported a new realtime collagen-remodeling models using the SHG microscope.　SHG microscope is based on a nonlinear optical effect known as second-harmonic generation and can image collagen structures without staining. In this project, we use the multi-well culture slides(μ-Slide Angiogenesis, ibidi) to evaluate the effect of multi proteins on collagen remodeling at once. We evaluated the effect of occular proliferation-associated proteins including periostin and tenacin C on the collagen remodeling. We also revealed that periostin and tenacin C promoted the retinal cell(retinal endothelial cell and retinal pigment epithelial cell) migration, proliferation and tube-formation.

Research Products

• [Journal Article] Microarray analysis of gene expression in fibrovascular membranes excised from patients with proliferative diabetic retinopathy. (2015)
  • Author(s): Ishikawa K, Yoshida S, Kobayashi Y, Zhou Y, Nakama T, Nakao S, Sassa Y, Oshima Y, Niiro H, Akashi K, Kono T, Ishibashi T.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 56(2) Pages: 932-46
  • Peer Reviewed
• [Journal Article] Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy: possible involvement of periostin. (2015)
  • Author(s): Kobayashi Y, Yoshida S, Nakama T, Zhou Y, Ishikawa K, Arita R, Nakao S, Miyazaki M, Sassa Y, Oshima Y, Izuhara K, Kono T, Ishibashi T
  • Journal Title: Br J Ophthalmol. Volume: 99(4) Pages: 451-6
  • Peer Reviewed
• [Journal Article] Inhibition of choroidal fibrovascular membrane formation by new class of RNA interference therapeutic agent targeting periostin (2015)
  • Author(s): Takahito Nakama, Shigeo Yoshida, Keijiro Ishikawa, Yoshiyuki Kobayashi, Yedi Zhou, Shintaro Nakao, Yukio Sassa, Yuji Oshima, Kazumasa Takao, Akiko Shimahara, Kazunori Yoshikawa, Tomohiro Hamasaki, Tadaaki Ohgi, Hirotake Hayashi, Akira Matsuda, Akira Kudo, Miho Nozaki, Yuichiro Ogura, Masahiko Kuroda, Tatsuro Ishibashi
  • Journal Title: Gene Therapy Volume: 22(2) Pages: 127-37
  • Peer Reviewed
• [Journal Article] 糖尿病患者の管理 (網膜症） (2015)
  • Author(s): 向野利寛、佐々 由季生
  • Journal Title: 臨床と研究 Volume: ９２（１） Pages: 63-68
  • Peer Reviewed
• [Journal Article] 増殖糖尿病網膜症に対する硝子体手術10年後視力予後因子の統計学的検討 (2015)
  • Author(s): 佐々 由季生、秋山 雅人、吉田茂生、佐伯 有祐、高橋 理恵 光武 智子 向野 利寛
  • Journal Title: 臨床眼科 Volume: -
  • Peer Reviewed
• [Journal Article] Increased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy: possible association with postoperative macular oedema. (2015)
  • Author(s): Yoshida S, Kubo Y, Kobayashi Y, Zhou Y, Nakama T, Yamaguchi M, Tachibana T, Ishikawa K, Arita R, Nakao S, Sassa Y, Oshima Y, Kono T, Ishibashi T.
  • Journal Title: Br J Ophthalmol Volume: -
  • Peer Reviewed
• [Journal Article] Ultrastructural changes of the vitreoretinal interface during long-term follow-up after removal of the internal limiting membrane. (2014)
  • Author(s): Toshio Hisatomi, Shoji Notomi, Takashi Tachibana, Yukio Sassa, Yasuhiro Ikeda, Takao Nakamura, Akifumi Ueno, Hiroshi Enaida, Toshinori Murata, Taiji Sakamoto, Tatsuro Ishibashi
  • Journal Title: American Journal of Ophthalmology Volume: 158(3) Pages: 550-6
  • Peer Reviewed
• [Journal Article] 眼疾患と遺伝子 ゲノムワイド遺伝子発現解析による眼内増殖性疾患の責任遺伝子同定と治療への展開 (2014)
  • Author(s): 吉田 茂生, 石橋 達朗, 石川 桂二郎, 佐々 由季生, 中尾 新太郎, 喜多 岳志, 有田 量一, 荒川 聡, 安里 良, 中間 崇仁, 有馬 充, 秋山 雅人, 小林 義行, 周 也荻, 衛藤 雅予
  • Journal Title: 日本眼科学会雑誌 Volume: 118/３ Pages: 241-282
  • Peer Reviewed / Open Access
• [Journal Article] Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy. (2014)
  • Author(s): Ishikawa K, Yoshida S, Nakao S, Nakama T, Kita T, Asato R, Sassa Y, Arita R, Miyazaki M, Enaida H, Oshima Y, Murakami N, Niiro H, Ono J, Matsuda A, Goto Y, Akashi K, Izuhara K, Kudo A, Kono T, Hafezi-Moghadam A, Ishibashi T.
  • Journal Title: FASEB J Volume: 28 Pages: 131-42
  • Peer Reviewed
• [Journal Article] 眼疾患と遺伝子 ゲノムワイド遺伝子発現解析による眼内増殖性疾患の責任遺伝子同定と治療への展開 (2014)
  • Author(s): 吉田 茂生, 石橋 達朗, 石川 桂二郎, 佐々 由季生, 中尾 新太郎, 喜多 岳志, 有田 量一, 荒川 聡, 安里 良, 中間 崇仁, 有馬 充, 秋山 雅人, 小林 義行, 周 也荻, 衛藤 雅予, 藤澤 公彦, 江内田 寛, 大島 裕司, 武田 篤信, 宮崎 勝徳, 安田 美穂, 吉村 武, 村上 祐介, 平川 沙弥香, 立花 崇 等
  • Journal Title: 日本眼科学会雑誌 Volume: 118 Pages: 241-282
  • Peer Reviewed
• [Journal Article] Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes. (2013)
  • Author(s): Asato R, Yoshida S, Ogura A, Nakama T, Ishikawa K, Nakao S, Sassa Y, Enaida H, Oshima Y, Ikeo K, Gojobori T, Kono T, Ishibashi T.
  • Journal Title: PLoS One Volume: 8(1)
  • NAID: 120005227330
  • Peer Reviewed
• [Journal Article] Comparison of Gene Expression Profile of Epiretinal Membranes Obtained from Eyes with Proliferative Vitreoretinopathy to That of Secondary Epiretinal Membranes (2013)
  • Author(s): Asato R, Yoshida S,Ogura A, Nakama T,Ishikawa K, Nakao S, Sassa Y, Enaida H, Oshima Y, Ikeo K, Gojobori T, Kono T, Ishibashi T
  • Journal Title: Plos One Volume: 10
  • NAID: 120005227330
  • Peer Reviewed
• [Journal Article] 毛様体扁平部濾過手術を施行した血管新生緑内障の術後成績の検討 (2012)
  • Author(s): 佐伯有祐 佐々由季生 安里 良 向野利寛
  • Journal Title: 眼科手術 Volume: 25 Pages: 577-581
  • Peer Reviewed
• [Journal Article] Involvement of periostin in regression of hyaloid vascular system during ocular development (2012)
  • Author(s): Arima M, Yoshida S, Nakama T, Ishikawa K, Nakao S, Yoshimura T, Asato R, Sassa Y, Kita T, Enaida H, Oshima Y, Matsuda A, Kudo A, Ishibashi T.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53 Pages: 6495-503
  • Peer Reviewed
• [Journal Article] Aniti-angiogenic shift in vitreous after vitrectomy in patients with proliferative diabetic retinopathy (2012)
  • Author(s): Yoshida S, Nakama T, Ishikawa K, Arima M, Tachibana T, Nakao S, Sassa Y, Yasuda M, Enaida H, Oshima Y, Kono T, Ishibashi T.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53 Pages: 6997-7003
  • Peer Reviewed
• [Presentation] 増殖糖尿病網膜症術前・術後因子の術10年後視力へ与える影響について統計学的解析 (2014)
  • Author(s): 佐々由季生、佐伯有祐、高橋理恵、光武智子、向野利寛
  • Organizer: 第53回日本網膜硝子体学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] 網膜剥離症例に対するHeavySILTMの使用経験 (2014)
  • Author(s): 佐々 由季生 高橋 理恵 光武 智子 佐伯 有祐 向野 利寛
  • Organizer: 第41回 福岡大学眼科研究会
  • Place of Presentation: 福岡
  • Year and Date: 2014-05-17
• [Presentation] The significant relationships between Fibronectin, Tenascin-C and Periostin in the eyes of the patients with diabetic retinopathy (2013)
  • Author(s): Yukio Sassa; Shigeo Yoshida; Keijiro Ishikawa; Ryo Asato; Mitsuru Arima; Masanori Miyazaki; Hiroshi Enaida; Yuji Oshima; Toshihiro Kono; Tatsuro Ishibashi
  • Organizer: ARVO annual meeting
  • Place of Presentation: Seatle, U.S.A
• [Presentation] Increased Concentrations of Periostin in the Vitreous of Proliferative Diabetic Retinopathy Patients with Tractional Retinal Detachment. (2013)
  • Author(s): Rie Takahashi, Yukio Sassa, Shigeo Yoshida, Yusuke Saeki, Tatsuro Ishibashi, Toshihiro Kono
  • Organizer: The 8th Asia Pacific Vitreo-Retina Society Congress
  • Place of Presentation: Nagoya, Ja
• [Presentation] ヒト増殖膜中のコラーゲンと平骨筋様細胞の分布の可視化 (2013)
  • Author(s): 石原七海、佐々由季生、福島修一郎、荒木勉
  • Organizer: 日本機械学会 第24回バイオフロンティア講演会
  • Place of Presentation: 同志社大学、京都市
• [Presentation] 第2高調波発生光（SHG光）顕微鏡を用いたコラーゲン線維再構築の経時的変化 (2012)
  • Author(s): 佐々 由季生、吉田 茂生、福島 修一郎、斉藤 徹也、田仲 亮介、藤澤 一樹、石川 桂二郎、安里 良、有馬 充、向野 利寛、安井 武史、荒木 勉、石橋 達朗
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] New method for evaluating 3D collagen remodeling by SHG microscope (2012)
  • Author(s): Yukio Sassa, Shigeo Yoshida , Shuichiro Fukushima , Tetsuya Saito, Keijiro Ishikawa, Toshihiro Kono, Tatsuro Ishibashi, Takeshi Yasui, Tsutomu Araki
  • Organizer: The 5th Joint Meeting of Korea-China-Japan Ophthalmologists
  • Place of Presentation: ソウル
• [Book] Diabetic Retinopathy: Fibrovascular membranes associated with PDR: Development of molecular targets by global gene expression profiling (2013)
  • Author(s): Shigeo Yoshida, Keijiro Ishikawa, Mitsuru Arima, Ryo Asato, Yukio Sassa and Tatsuro Ishibashi
  • Publisher: INTECH
• [Book] Advances in Medicine and Biology. Volume 53 (2012)
  • Author(s): Yukio Sassa, Atsunobu Takeda, Shigeo Yoshida, Yo-ichi Kawano, Toshihiro Kono, Tatsuro Ishibashi
  • Publisher: Nova Science Publishers, Inc
• [Patent(Industrial Property Rights)] 第二次高調波光を用いた新規コラーゲン線維化評価モデル (2012)
  • Inventor(s): 佐々 由季生
  • Industrial Property Rights Holder: 佐々 由季生
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-186951
  • Filing Date: 2012-08-27