Interaction between miR-21 and TGFbeta: Role in chronic non-healing wounds

Research Project

Project/Area Number	24592716
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Plastic surgery
Research Institution	University of Miyazaki
Principal Investigator	MADHYASTHA Radha 宮崎大学, 医学部, 助教 (80381078)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	創傷治癒 / MicroRNA / TGFb1 / microRNA
Outline of Final Research Achievements	This project aimed to investigate the interaction between TGFβ1 and microRNA miR-21 in diabetic wounds, and to evaluate the role of miR-21 in diabetic wound healing. Employing mouse skin fibroblast cells, we found that TGFβ1 enhances miR-21 expression in hyperglycemic conditions. TGFβ1 effect on miR-21 was dependent on NFkB induced ROS generation. MiR-21 was found to promote cell migration towards wound area. Analysis of diabetic fibroblast cells revealed that miR-21 was downregulated in these cells.

Report

(4 results)