Interaction between miR-21 and TGFbeta: Role in chronic non-healing wounds
Project/Area Number |
24592716
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plastic surgery
|
Research Institution | University of Miyazaki |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 創傷治癒 / MicroRNA / TGFb1 / microRNA |
Outline of Final Research Achievements |
This project aimed to investigate the interaction between TGFβ1 and microRNA miR-21 in diabetic wounds, and to evaluate the role of miR-21 in diabetic wound healing. Employing mouse skin fibroblast cells, we found that TGFβ1 enhances miR-21 expression in hyperglycemic conditions. TGFβ1 effect on miR-21 was dependent on NFkB induced ROS generation. MiR-21 was found to promote cell migration towards wound area. Analysis of diabetic fibroblast cells revealed that miR-21 was downregulated in these cells.
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Report
(4 results)
Research Products
(2 results)