Experimental study of induction of cytokines through skin and ulcer base using electroporation
| Project/Area Number |
24592724
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAKUDO Natsuko 関西医科大学, 医学部, 助教 (00509490)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | PRP / cytokine / electroporation / regenerative medicine / skin / skin ulcer / 電圧 / パルス |
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| Outline of Final Research Achievements |
Skin and basis of skin ulcer have protective function not to permeate through them for medications and materials over 20kD molecules in the size. It is aimed to treat skin wounds and aging skin broadly, if medications and materials with larger molecule can be penetrated. In this time, how to penetrate 20-100kD growth factors minimally invasively trough the skin and the base of the skin ulcer using electroporation (EP method) is studied.
Any invasions in EP method, and permeating bFGF and PRP, which contains various growth factors, were investigated invasions. As comparing in voltages, in 80V light skin stimulation was observed only during induction. No skin and soft tissue damages remained. Cytokines penetrating the skin surface was confirmed, however, more detailed mechanisms will be investigated in the future.
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Report
(4 results)
Research Products
(53 results)
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[Journal Article] Latent TGFβ binding protein-2 is essential for the development of ciliary zonule microfibrils.2014
Author(s)
Inoue T., Ohbayashi T., Fujikawa Y., Yoshida H., Akama T.O., Noda K., Horiguchi M., Kameyama K., Hata Y., Takahashi K., Kusumoto K. and Nakamura T.
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Journal Title
Human Molecular Genetics
Volume: 23
Issue: 21
Pages: 5672-5682
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Principles of PRP and the PRP therapy2014
Author(s)
Kusumoto K, Morimoto N, Fukuda S, Kakudo N, Miyake Y, Notodihardjo PV, Ogura T, Hara T.
Organizer
The 12th Korea-Japan Congress of Plastic and Reconstructive Surgery
Place of Presentation
Incheon, Korea, Incheon Convention Center
Year and Date
2014-05-17
Related Report
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[Presentation] 多血小板血漿（PRP）の意義とその展望2014
Author(s)
楠本健司, 森本尚樹, 井口有子, 覚道奈津子, Priscilla Valentin, 小倉常敬, 原 朋也
Organizer
第57回日本形成外科学会総会・学術集会
Place of Presentation
長崎市 長崎ブリックホール
Year and Date
2014-04-10
Related Report
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[Presentation] PRP療法の原理と調整について2014
Author(s)
覚道奈津子, 森本尚樹, Notodihardjo Priscilla Valentin, 小倉常敬, 原 朋也, 楠本健司
Organizer
第57回日本形成外科学会総会・学術集会
Place of Presentation
長崎市 長崎ブリックホール
Year and Date
2014-04-10
Related Report
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[Presentation] Autologous platelet-rich plasma(PRP) and the application for various wounds and poor conditions
Author(s)
Kusumoto K, Suzuki K, Fukuda S, Kakudo N, Tanaka Y, Sasao T, Miyake Y, Kushida S, Priscilla V, Ogura T.
Organizer
The 11th Japan-Korea Congress of Plastic and Reconstructive Surgery
Place of Presentation
Awaji-kokusai-yumebutai-kaigijyou, Awaji island, Japan（国内）
Related Report
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[Presentation] Autologous Platelet-Rich Plasma(PRP) and the application for chronic wounds
Author(s)
Kenji Kusumoto, Kenji Suzuki, Satoshi Fukuda, Natsuko Kakudo, Yoshihito Tanaka, Takashi Sasao, Yoshikazu Miyake, Satoshi Kushida, Priscilla Valentin Notodihardjo, Tsunetaka Ogura
Organizer
the 4th Congress of the World Union of Wound Healing Societies
Place of Presentation
Pacifico Yokohama, Yokohama, Japan（国内）
Related Report
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[Presentation] An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers
Author(s)
Naoki Morimoto, Tatsuya Ito, Norikazu kanda, Katsuya Kawai, Yoko Nakamura, Satoshi Teramukai, Masayuki Yokode, Taira Maekawa, Akira Shimizu, Shigehiko Suzuki
Organizer
the 4th Congress of the World Union of Wound Healing Societies
Place of Presentation
Pacifico Yokohama, Yokohama, Japan（国内）
Related Report
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