The study of the repair mechanism by neuron and glial cell after central nervous system injury

• Project/Area Number: 24592729
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Fukushima Medical University (2013-2014); Yamagata University (2012)
• Principal Investigator: ISEKI Ken 福島県立医科大学, 医学部, 教授 (70332921)
• Co-Investigator(Kenkyū-buntansha): GOTO Kaoru 山形大学, 医学部, 教授 (30234975)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: グリオーシス / 凍結脳損傷 / プロテオグリカン / 脂質代謝酵素 / 肝障害 / 核内脂質代謝酵素 / コンドロイチン硫酸プロテオグリカン / ヘパラン硫酸プロテオグリカン / 中枢神経 / アストロサイト / 脳損傷 / ニューロン / グリア

Outline of Final Research Achievements

Transcription factor OASIS controls the production of proteoglycan in embryonic development, whereas Olig is a key factor of glial differentiation. However, detailed repair mechanism after brain injury remains unclear. We investigated the expression pattern of these transcription factors after brain injury. Results suggest that OASIS is involved in the regulatory mechanism of non-permissive environments for axonal outgrowth and that Olig genes regulate glial differentiation. The expression pattern of the relevant molecules remained unchanged in KO mice of diacylglycerol kinase, an enzyme responsible for the control of a lipid messenger diacylglycerol.

Research Products

• [Journal Article] Role of the N-terminal hydrophobic residues of DGKε in targeting the endoplasmic reticulum. (2014)
  • Author(s): Matsui H, Hozumi Y, Tanaka T, Okada M, Nakano T, Suzuki Y, Iseki K, Kakehata S, Topham MK, Goto K.
  • Journal Title: Biochim Biophys Acta Molecular and Cell Biology of Lipids Volume: 1842 Issue: 10 Pages: 1440-1450
  • DOI: 10.1016/j.bbalip.2014.07.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] An Outbreak of Carbon Monoxide Poisoning in Yamagata Prefecture Following the Great East Japan Earthquake (2013)
  • Author(s): Iseki K, Hayashida A, Shikama Y, Goto K, Tase C
  • Journal Title: Asia Pac J of Med Toxicol Volume: 2 Pages: 37-41
  • Peer Reviewed
• [Journal Article] Gliosis-specific transcription factor OASIS coincides with proteoglycan core protein genes in the glial scar and inhibits neurite outgrowth (2012)
  • Author(s): Iseki K, Hagino S, Nikaido T, Zhang Y, Mori T, Yokoya S, Hozumi Y, Goto K, Wanaka A, Tase C
  • Journal Title: Biomedical Research Volume: 33 Pages: 345-353
  • NAID: 130004903839
  • Peer Reviewed
• [Journal Article] NMDA receptor-mediated Ca(2+) influx triggers nucleocytoplasmic translocation of diacylglycerol kinase ζ under oxygen-glucose deprivation conditions, an in vitro model of ischemia, in rat hippocampal slices (2012)
  • Author(s): Suzuki Y, Yamazaki Y, Hozumi Y, Okada M, Tanaka T, Iseki K, Ohta N, Aoyagi M, Fujii S, Goto K.
  • Journal Title: Histochem CellBiol Volume: 137 Issue: 4 Pages: 499-511
  • DOI: 10.1007/s00418-011-0907-y
  • Peer Reviewed
• [Presentation] 一酵素ファミリーから見た生命現象 (2014)
  • Author(s): 後藤薫
  • Organizer: 日本解剖学会第60回東北・北海道連合支部学術集会
  • Place of Presentation: 福島学院大学
  • Year and Date: 2014-09-06 – 2014-09-07
  • Invited
• [Presentation] ζ型ジアシルグリセロールキナーゼ欠損マウスにおける臓器特異的糖脂質代謝異常と肥満について (2014)
  • Author(s): 中野知之、後藤薫
  • Organizer: 日本解剖学会第60回東北・北海道連合支部学術集会
  • Place of Presentation: 福島学院大学
  • Year and Date: 2014-09-06 – 2014-09-07
• [Presentation] ベータ型ジアシルグリセロールキナーゼはGluR2を介して線条体投射ニューロンの棘突起形成に関与する (2014)
  • Author(s): 八月朔日泰和、後藤薫
  • Organizer: 日本解剖学会第60回東北・北海道連合支部学術集会
  • Place of Presentation: 福島学院大学
  • Year and Date: 2014-09-06 – 2014-09-07
• [Presentation] An Overview of Hydrogen Sulfide Poisoning in Yamagata Prefecture (2013)
  • Author(s): Iseki K, Hayashida A, Yamazaki K, Tase C
  • Organizer: 12th Asia Pacific Association of Medical Toxicology
  • Place of Presentation: ドバイ
• [Presentation] Subcellular localization of Diacylglycerol kinase ε in transfected neurons (2012)
  • Author(s): Hirooki Matsui, Yasukazu Hozumi, Ken Iseki, Kaoru Goto
  • Organizer: 42th Annual Meeting of Neuroscience
  • Place of Presentation: Convention Center, New Orleans, USA