Functional analysis of Hippo signaling pathway during salivary gland development in mice.

Research Project

Project/Area Number	24592768
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Morphological basic dentistry
Research Institution	Hiroshima University
Principal Investigator	MICHINORI Kitagawa 広島大学, 医歯薬保健学研究院(歯), 助教 (30314496)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	唾液腺 / 発生 / シグナル伝達 / 細胞増殖 / Hippo / 管腔構造
Outline of Final Research Achievements	The aim of this study is to clarify the role of Hippo signal transduction pathway for cell growth and maintenance of luminal structure in mouse salivary gland tissue. The analysis of gene expression of Hippo factors showed that transcription co-factor Yap protein localized in different manner between embryo and adult salivary gland cells. Inhibition of Yap protein translation decreased growth of salivary gland tissue and branching of epithelium in tissue culture system. The results suggest that Yap has an important role in growth of salivary gland tissue.