| Project/Area Number |
24592771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
MIYAZAKI Toshihiro 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10174161)
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| Co-Investigator(Kenkyū-buntansha) |
MORIISHI Takeshi 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (20380983)
BABA Tomomi 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (60189727)
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| Co-Investigator(Renkei-kenkyūsha) |
KOMORI Toshihisa 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タウ蛋白質 / 象牙芽細胞 / Runx2 / マイクロアレイ / 免疫組織化学 / 細胞分化 / 突起形成 / チューブリン / 細胞骨格 / 骨芽細胞 / 免疫電子顕微鏡 / 象牙芽細胞突起 / 骨芽細胞突起 / 免疫電顕 / マイクロアレー |
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| Outline of Final Research Achievements |
The aim of this study is to examine the mechanism of the formation of processes in the osteoblasts and odontoblasts. We have demonstrated using microarray and Real-time PCR analyses that microtubule-associated protein tau (Mapt), a neuronal phosphoprotein, is highly expressed in wild-type mouse molars but its expression is severely reduced in Tg(Col1a1-Runx2) mouse molars, in which odontoblasts lose their characteristic polarized morphology including a long process extending to dentin. Furthermore, immunohistochemical analysis showed that Mapt was exclusively localized in terminally differentiated odontoblasts including their processes in wild-type molars and was ultrastructurally present around the α-tubulin-positive filamentous structures. This result indicates that Mapt may be an important factor for odontoblast morphogenesis and is a useful marker protein for evaluating the terminal differentiation of odontoblasts in mice.
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