Analysis of primary hematopoiesis in the adult mice

• Project/Area Number: 24592777
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: Nakamura Masanori 昭和大学, 歯学部, 教授 (50180394)
• Co-Investigator(Kenkyū-buntansha): NONAKA Naoko 昭和大学, 歯学部, 准教授 (20307052)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 造血 / 一次造血 / ビスプォスプォネート / 髄外造血 / 肝臓 / hemal node / 骨髄 / hamal node / bisphosphonate / 脾臓 / hamel node / グロビン

Outline of Final Research Achievements

The treatment with nitrogen-containing bisphosphonate (NBP) and phenylhydrazine (PHZ) into mice enhanced the extramedullary hematopoiesis in the liver and induced the appearance of nuclear erythrocytes in the peripheral blood and hemal node like structure in the abdominal cavity. The gene analysis of hemoglobin showed the appearance of primary hematopoiesis in the bone marrow, liver and hemal node like structure inaddition to the secondary hematopoiesis. Our results first indicated the switch from the secondary to primary hematopoiesis in normal genomic animal model.

Research Products

• [Journal Article] he expression of embryonic globin mRNA in a severely anemic mouse model induced by treatment with nitrogen-containing bisphosphonate (2016)
  • Author(s): Otsuka H, Takito J, Endo Y, Yagi H, Soeta S, Nonaka N, Nakamura M
  • Journal Title: BMC Hematol Volume: 16 Issue: 1 Pages: 4-4
  • DOI: 10.1186/s12878-016-0041-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Analysis of periapical alveolar bone resorption after the removal of interdental wire ligation (2015)
  • Author(s): Kubono Y, Mayahara M, Otsuka H, Kataoka R, Takagi Y, Asari J, Nonaka N, Inoue M, Nakamura M
  • Journal Title: Ped Dent J Volume: 25 Issue: 1 Pages: 1-7
  • DOI: 10.1016/j.pdj.2014.12.002
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regulation of Osteoclast Multinucleation by the Actin Cytoskeleton Signaling Network. (2014)
  • Author(s): Takito J, Otsuka H, Yanagisawa N, Arai H, Shiga M, Inoue M, Nonaka N, Nakamura M
  • Journal Title: J Cell Physiol Volume: 230 Issue: 2 Pages: 395-405
  • DOI: 10.1002/jcp.24723
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NKG2D+ IFN-γ+ CD8+ T cells are responsible for palladium allergy. (2014)
  • Author(s): Kawano M, Nakayama M, Aoshima Y, Nakamura K, Ono M, Nishiya T, Nakamura S, Takeda Y, Dobashi A, Takahashi A, Endo M, Ito A, Ueda K, Sato N, Higuchi S, Kondo T, Hashimoto S, Watanabe M, Watanabe M, Takahashi T, Sasaki K, Nakamura M, Sasazuki T, Narushima T, Suzuki R, Ogasawara K.
  • Journal Title: PLoS One. Volume: 9 Issue: 2 Pages: 1-12
  • DOI: 10.1371/journal.pone.0086810
  • Peer Reviewed / Open Access
• [Journal Article] Composition of the blood supply in the sublingual and submandibular spaces and its relationship to the lateral lingual foramen of the mandible. (2014)
  • Author(s): Nakajima K, Tagaya A, Otonari-Yamamoto M, Seki K, Araki K, Sano T, Okano T, Nakamura M
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Volume: 117 Issue: 1 Pages: e32-e38
  • DOI: 10.1016/j.oooo.2012.03.032
  • Peer Reviewed
• [Journal Article] Mast cell maturation is driven via a novel group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis. (2013)
  • Author(s): Taketomi, Y., Morimoto, K., Murakami, M., et al., 他30名
  • Journal Title: Nature Immunol. Volume: (印刷中) Issue: 6 Pages: 554-63
  • DOI: 10.1038/ni.2586
  • Peer Reviewed
• [Journal Article] Calcified tissue formation of subcutaneously transplanted mouse dental pulp. (2012)
  • Author(s): Ikeda-Isogai M, Ohtsuka T, Baba K, Nonaka N, Nakamura M.
  • Journal Title: Acta Histochem Volume: 114(1) Issue: 1 Pages: 55-61
  • DOI: 10.1016/j.acthis.2011.02.007
  • Peer Reviewed
• [Journal Article] The transient appearance of zipper-like actin superstructures during the fusion of osteoclasts. (2012)
  • Author(s): Takito J, Nakamura M, Yoda M, Tohmonda T, Uchikawa S, Horiuchi K, Toyama Y, Chiba K
  • Journal Title: J Cell Sci Volume: 125 Issue: 3 Pages: 662-672
  • DOI: 10.1242/jcs.090886
  • Peer Reviewed
• [Journal Article] Linked precursors via the zipper-like structure or the filopodium during the secondary fusion of osteoclasts. (2012)
  • Author(s): Takito J, Nakamura M
  • Journal Title: Communicative & Integrative Biology Volume: 5 Issue: 5 Pages: 450-454
  • DOI: 10.4161/cib.20980
  • Peer Reviewed
• [Journal Article] Role of the junctional epithelium in periodontal innate defense and homeostasis. (2012)
  • Author(s): Tsukamoto Y, Usui M, Yamamoto G, Takagi Y, Tachikawa T, Yamamoto M, Nakamura M.
  • Journal Title: J Periodontal Res Volume: 47(6) Issue: 6 Pages: 750-7
  • DOI: 10.1111/j.1600-0765.2012.01490.x
  • Peer Reviewed
• [Journal Article] Intranasal administration of PACAP: uptake by brain and regional brain targeting with cyclodextrins. (2012)
  • Author(s): Nonaka Naoko
  • Journal Title: Peptides Volume: 36 Issue: 2 Pages: 168-175
  • DOI: 10.1016/j.peptides.2012.05.021
  • Peer Reviewed
• [Journal Article] Involvement of intestinal intraepithelial lymphocytes in turnover of intestinal epithelial cells -Morphological and functional alterations due to daily administration of FK506-. (2012)
  • Author(s): Hidaka M, Nakamura M, Ohmichi Y, Itoh J, Fukuzawa K, Masuko T, Yagi H
  • Journal Title: Cell Immunol Volume: 276 Issue: 2 Pages: 124-133
  • DOI: 10.1016/j.cellimm.2012.10.007
  • Peer Reviewed
• [Presentation] 加齢マウス唾液腺におけるリンパ球浸潤と十全大補湯によるその抑制効果 (2016)
  • Author(s): 野中直子，中村雅典
  • Organizer: 第15回日本抗加齢医学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2016-05-29
• [Presentation] 十全大補湯による加齢マウス唾液腺におけるリンパ球浸潤抑制効果 (2016)
  • Author(s): 野中直子，中村雅典
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 郡山
  • Year and Date: 2016-03-28
• [Presentation] 長骨骨幹部および骨幹端における骨修復機構の比較 (2016)
  • Author(s): 井上知、大塚裕忠、瀧戸次郎、柳澤伸彰、野中直子、中村雅典
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 郡山
  • Year and Date: 2016-03-28
• [Presentation] ミノドロネート投与によるコラーゲン誘導型関節炎モデルへの影響 (2016)
  • Author(s): 新井宏、大塚裕忠、瀧戸次郎、井上知、栁澤伸彰、野中直子、中村雅典.
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 郡山
  • Year and Date: 2016-03-28
• [Presentation] 破骨細胞に見られるアクチン超構造体の静的構造 (2016)
  • Author(s): 瀧戸二郎、中村雅典
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 郡山
  • Year and Date: 2016-03-28
• [Presentation] 窒素含有型ビスホスホネートを用いた重症貧血マウスモデルにおける新規造 血構造誘導機構の解析 (2016)
  • Author(s): 大塚裕忠，中村雅典
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 郡山
  • Year and Date: 2016-03-28
• [Presentation] Effect of Minodronate on collagen-induced arthritis model mice (2016)
  • Author(s): Hiroshi Arai, Hirotada Otsuka , Jiro Takito , Satoshi Inoue , Nobuaki Yanagisawa , Naoko Nonaka, Masanori Nakamura
  • Organizer: APICA
  • Place of Presentation: Singapore
  • Year and Date: 2016-03-17
  • Int'l Joint Research
• [Presentation] The suppressant effect of lymphocyte infiltration by Juzentaihoto in the aging mouse salivary glands. (2015)
  • Author(s): Naoko Nonaka, Masanori Nakamura
  • Organizer: GSA2015 Annual Scientific Meeting
  • Place of Presentation: Orlando, USA
  • Year and Date: 2015-11-18
  • Int'l Joint Research
• [Presentation] The anatomy of the zipper-like structure (2015)
  • Author(s): Jiro Takito and Masanori Nakamura
  • Organizer: 63rd Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Fukuoka
  • Year and Date: 2015-10-30
• [Presentation] Change of PACAP receptor expressing cells during the development of granular duct in male mouse submandibular glands (2015)
  • Author(s): Naoko Nonaka, Masanori Nakamura
  • Organizer: 2015 Experimental Biology
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-26 – 2015-04-01
• [Presentation] Dynamic change of PACAP receptor with the development of granular ducts in male mouse submandibular glands (2015)
  • Author(s): Naoko Nonaka, Seiji Shioda, Masanori Nakamura
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The Effect of Minodronate on Murine Hematopoiesis (2015)
  • Author(s): Inoue S, Ohtsuka H, Arai H, Nakamura M
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Effect of Nitrogen-containing bisphosphonates on collagen-induced arthritis model mice (2015)
  • Author(s): Arai H, Otsuka H, Nakamura M
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The study of fat associated lymphoid clusters and hemal node-like structures in critical anemic mice (2015)
  • Author(s): Otsuka H, Takito J, Nonaka N, Nakamura M
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Dissociation of the effect of Rho agonist on endocytosis from that on cell fusion in RAW 264.7 cells (2015)
  • Author(s): Takito J, Kawashima T, Nakamura M
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 雄性マウス顎下腺における顆粒性導管の出現とPACAPレセプターの局在変化について (2014)
  • Author(s): 野中直子，中村雅典
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Immunohistochemical localization of PACAP receptor in developing mouse salivary glands (2014)
  • Author(s): Yuko Matoba, Naoko Nonaka, Eisaku Imamura, Masanori Nakamura
  • Organizer: 11th Asian Congress on Oral and Maxillofacial Surgery
  • Place of Presentation: Xi’an, China
  • Year and Date: 2014-08-22 – 2014-08-25
• [Presentation] Localization of PACAP receptors in mouse major salivary glands with aging (2014)
  • Author(s): Naoko Nonaka, Masanori Nakamura.
  • Organizer: IFAA
  • Place of Presentation: Beijing, China
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] PACAPによる唾液分泌効果と加齢に伴うPACAPレセプター局在の解析 (2014)
  • Author(s): 野中直子，中村雅典
  • Organizer: 第14回日本抗加齢医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-06-06 – 2014-06-08
• [Presentation] マウス唾液腺の発生過程におけるPACAPレセプター局在の免疫組織学的解析 (2014)
  • Author(s): 野中直子、中町智哉、塩田清二、中村雅典
  • Organizer: 第119回 日本解剖学会学術大会・総会
  • Place of Presentation: 宇都宮
• [Presentation] アクチン細胞骨格を制御するシグナルネットワークによる破骨細胞多核化の調節 (2014)
  • Author(s): 瀧戸二郎、中村雅典
  • Organizer: 第119回 日本解剖学会学術大会・総会
  • Place of Presentation: 宇都宮
• [Presentation] Regulation of osteoclast multinucleation by the signaling network of actin cytoskeleton (2013)
  • Author(s): Jiro Takito, Hirotada Otsuka, Nobuaki Yanagisawa, Hiroshi Arai, Masayasu Shiga, Mitsuko Inoue, Naoko Nonaka, Masanori Nakamura
  • Organizer: Annual Meeting of The American Society for Cell Biology
  • Place of Presentation: New Orleans
• [Presentation] Histological and immunohistochemical comparison of major salivary glands in young and old mice. (2013)
  • Author(s): Naoko Nonaka, Masanori Nakamura
  • Organizer: Experimental Biology 2013
  • Place of Presentation: Boston
• [Presentation] 人体解剖学実習におけるグループダイナミックス評価の検討その２ (2013)
  • Author(s): 野中直子、中島功、片岡竜太、中村雅典
  • Organizer: 第32回日本歯科医学教育学会総会および学術大会
  • Place of Presentation: 札幌
• [Presentation] 顎関節の発生と加齢変化 (2013)
  • Author(s): 中村雅典
  • Organizer: 第31回 日本顎咬合学会学術大会・総会
  • Place of Presentation: 東京
  • Invited
• [Presentation] マウス唾液腺の発生過程におけるPACAPレセプター（PAC1R）局在の解析 (2013)
  • Author(s): 野中直子、中村雅典
  • Organizer: 第55回 歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
• [Presentation] PACAPによる唾液分泌効果とレセプター局在の解析 (2013)
  • Author(s): 野中直子、中町智哉、塩田清二、中村雅典
  • Organizer: 日本解剖学会関東支部第101回学術集会
  • Place of Presentation: 東京
• [Presentation] マウス唾液腺の発生過程におけるPACAPレセプター（PAC1R）局在について (2013)
  • Author(s): 高木佳辰，野中直子，的場裕子，今村栄作，中村雅典
  • Organizer: 第33回昭和歯学会例会
  • Place of Presentation: 東京
• [Presentation] ウシ骨由来移植材の吸収過程の解析 (2013)
  • Author(s): 新井宏,栁澤伸彰,鈴木治,中村雅典
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
• [Presentation] 加齢に伴うマウス唾液腺における構造的機能的変化の解析 (2013)
  • Author(s): 野中直子、中町智哉、塩田清二、中村雅典
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松
• [Presentation] 成体マウスにおける胚子ヘモグロビンの再発現について (2013)
  • Author(s): 大塚裕忠、中村雅典
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松
• [Presentation] 破骨細胞融合時に出現する細胞間アクチン超構造体 (2013)
  • Author(s): 瀧戸二郎、中村雅典
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松
• [Presentation] Histological and Immunohisotchemical comparison of mouse salivary glands in aging. (2013)
  • Author(s): Nonaka N, Nakamura M
  • Organizer: 91th International Association of Dentak Research
  • Place of Presentation: シアトル
• [Presentation] Intranasal Delivery of PACAP: Targeting to Brain Regions Beyond the Olfactory Bulb. (2012)
  • Author(s): Nonaka N, Nakamachi N,Nakamura M, Shioda S, Banks WA
  • Organizer: The 3rd Meeting of the Japan Branch of the International Neuropeptide Society
  • Place of Presentation: 北九州
  • Invited
• [Presentation] 加齢に伴うマウス唾液腺におけるPACAPレセプター局在の解析 (2012)
  • Author(s): 野中直子、中村雅典
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
• [Book] 組織学・口腔組織学<第4版> (2014)
  • Author(s): 磯川桂太郎・稲井哲一朗・中村雅典・山本仁・渡邊弘樹
  • Total Pages: 448
  • Publisher: わかば出版
• [Book] 歯の解剖学 (2014)
  • Author(s): 近藤信太郎・中村雅典・松野昌展
  • Total Pages: 160
  • Publisher: わかば出版
• [Book] ネッター頭頸部・口腔顎顔面の臨床解剖アトラス<原著第2版> (2014)
  • Author(s): 前田健康 監訳
  • Total Pages: 688
  • Publisher: 医歯薬出版
• [Book] The Functional Diversity of Kupffer Cells. In Handbook of Macrophages: Life Cycle, Functions and Diseases (2012)
  • Author(s): Nakamura M, Otsuka Y, Yagi H, Endo Y
  • Total Pages: 16
  • Publisher: Nova Science Publishers
• [Book] Extramedullary erythropoiesis in anemia. In The New Frontiers in Research for Oral Cancer. (2012)
  • Author(s): Nakamura M, Otsuka H, Nonaka N, Yanagisawa N, Yagi H, Endo Y
  • Total Pages: 11
  • Publisher: MARUZEN PLANET