| Project/Area Number |
24592786
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
NINOMIYA Tadashi 松本歯科大学, 総合歯科医学研究所, 講師 (00360222)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Hiroaki 松本歯科大学, 歯学部, 教授 (50227930)
HIRAGA Toru 松本歯科大学, 歯学部, 准教授 (70322170)
KOIDE Masanori 松本歯科大学, 総合歯科医学研究所, 講師 (10367617)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ラクトフェリン / 骨芽細胞 / 破骨細胞 / 細胞分化 / 好中球 / 破骨細胞分化 / LRP-1 / 骨芽細胞分化 |
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| Outline of Final Research Achievements |
To elucidate the roles of lactoferrin for the bone metabolism, we examined the effects of bovine- (bLF) and neutrophil-derived lactoferrin (nLF) on osteoblast and osteoclast differentiation. In vitro studies using mesenchymal stromal cells (MSCs) showed that bLF and nLF promoted the mRNA expressions of osteoblast markers and increased a number of ALPase-positive cells. On the other hand, formation of TRAP-positive cell from bone marrow macrophages (BMMφ) and the mRNA expressions of osteoclast markers in BMMφ were inhibited by these LF. In addition, neutrophil-cultured conditioned medium accelerated osteoblast differentiation of MSCs and inhibited osteoclast differentiation of BMMφ. Lipoprotein receptor related protein 1 as a receptor of lactoferrin expressed in BMMφ with the stimulation of RANKL and MSCs.
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