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Functional analysis of the molecular complex in spatiotemporal regulation of protein phosphorylation.

Research Project

Project/Area Number 24592805
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

TAKEUCHI HIROSHI  九州歯科大学, 歯学部, 教授 (70304813)

Co-Investigator(Renkei-kenkyūsha) GAO Jing  九州大学, 大学院歯学研究院, 助教 (40585882)
Research Collaborator ZHANG Zhao  
NAGANO Koki  
WANG Daguang  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsリン酸化 / 脱リン酸化 / 細胞内情報伝達 / 開口分泌 / 細胞内情伝達機構
Outline of Final Research Achievements

We have previously isolated a signaling molecule, PRIP which forms the protein complex with multiple kinases and phosphatases. In this year, we identified that (a)the C2 domains of PRIP and those of other molecules form hetero-dimers, and (b)a PRIP-interacting kinase Akt regulates exocytosis by phosphorylation of its new substrate tomosyn.
The results obtained in this study would promote understanding the mechanism how the phospho-dependent cellular signaling is spatio-temporally regulated by the complex containing multiple enzymes responsible for protein phosphorylation/de-phosphorylation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (36 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 2 results,  Acknowledgement Compliant: 5 results) Presentation (23 results) Remarks (2 results)

  • [Journal Article] Promotion of insulin-induced glucose uptake in C2C12 myotubes by osteocalcin.2015

    • Author(s)
      Tsuka, S., Aonuma, F., Higashi, S., Ohsumi, T., Nagano, K., Mizokami, A., Kawakubo-Yasukochi, T., Masaki, C., Hosokawa, R. and Hirata, M. and Takeuchi, H.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 459 Issue: 3 Pages: 437-442

    • DOI

      10.1016/j.bbrc.2015.02.123

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tomosyn is a novel Akt substrate mediating insulin-dependent GLUT4 exocytosis.2015

    • Author(s)
      Nagano, K., Takeuchi, H., Gao, J., Mori, Y., Otani, T., Wang, D. and Hirata, M.
    • Journal Title

      The International Journal of Biochemistry & Cell Biology

      Volume: 62 Pages: 62-71

    • DOI

      10.1016/j.biocel.2015.02.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Signaling pathway for adiponectin expression in adipocytes by osteocalcin.2015

    • Author(s)
      Otani, T., Mizokami, A., Hayashi, Y., Gao, J., Mori, Y., Nakamura, S., Takeuchi, H. and Hirata, M.
    • Journal Title

      Cellular Signalling

      Volume: 27 Issue: 3 Pages: 532-544

    • DOI

      10.1016/j.cellsig.2014.12.018

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hetero-oligomerization of C2 domains of phospholipase C-related but catalytically inactive protein and synaptotagmin-1.2015

    • Author(s)
      Wang, D., Takeuchi, H., Gao, J., Zhang, Z. and Hirata, M.
    • Journal Title

      Advances in Biological Regulation

      Volume: 57 Pages: 120-129

    • DOI

      10.1016/j.jbior.2014.09.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Oral administration of osteocalcin improves glucose utilization by stimulating glucagon-like peptide-1 secretion.2014

    • Author(s)
      Mizokami, A., Yasutake, Y., Higashi, S., Kawakubo-Yasukochi, T., Chishaki, S., Takahashi, I., Takeuchi, H. and Hirata, M.
    • Journal Title

      Bone

      Volume: 69 Pages: 68-79

    • DOI

      10.1016/j.bone.2014.09.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PRIP (phospholipase C-related but catalytically inactive protein) inhibits exocytosis by direct interactions with syntaxin 1 and SNAP-25 through its C2 domain2013

    • Author(s)
      Zhang, Z.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288 Issue: 11 Pages: 7769-7780

    • DOI

      10.1074/jbc.m112.419317

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation2013

    • Author(s)
      Umebayashi, H.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 432 Issue: 2 Pages: 268-274

    • DOI

      10.1016/j.bbrc.2013.01.119

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteocalcin induces release of glucagon-like peptide-1 and thereby stimulates insulin secretion in mice.2013

    • Author(s)
      Akiko Mizokami, Yu Yasutake, Jing Gao, Miho Matsuda, Ichiro Takahashi, Hiroshi Takeuchi, Masato Hirata
    • Journal Title

      Plos ONE

      Volume: 8 Issue: 2 Pages: e57375-e57375

    • DOI

      10.1371/journal.pone.0057375

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phospholipase C-related but catalytically inactive protein, PRIP as a scaffolding protein for phospho-regulation2013

    • Author(s)
      Sugiyama et al
    • Journal Title

      Advances in Bioloical Regulation

      Volume: 53 Issue: 3 Pages: 331-340

    • DOI

      10.1016/j.jbior.2013.07.001

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulated interaction of protein phosphatase 1 and protein phosphatase 2A with phospholipase C-related but catalytically inactive protein.2012

    • Author(s)
      Goro Sugiyama, Hiroshi Takeuchi, Koki Nagano, Jing Gao, Yukiko Ohyama, Yoshide Mori, Masato Hirata
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 16 Pages: 3394-403

    • DOI

      10.1021/bi2018128

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phospholipase C-related but catalytically inactive protein (PRIP) modulates synaptosomal-associated protein 25 (SNAP-25) phosphorylation and exocytosis.2012

    • Author(s)
      Jing Gao, Hiroshi Takeuchi, Zhao Zhang, Mitsunori Fukuda and Masato Hirata
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 287 Issue: 13 Pages: 10565-10578

    • DOI

      10.1074/jbc.m111.294645

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Hetero-oligomerization of C2 domains of phospholipase C-related but catalytically inactive protein and synaptotagmin-1.2015

    • Author(s)
      Wang, D., Takeuchi, H., Gao, J., Zhang, Z., Hirata, M.
    • Organizer
      Kyudai Oral Bioscience 2015 -9th International Symposium-
    • Place of Presentation
      福岡リーセントホテル(福岡県福岡市)
    • Year and Date
      2015-02-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Osteocalcin modifies insulin-induced glucose-uptake in cultured myotube.2015

    • Author(s)
      Tsuka, S., Aonuma, F., Hosokawa, R., Hirata, M., Takeuchi, H.
    • Organizer
      APC2015
    • Place of Presentation
      九州歯科大学講堂(福岡県北九州市)
    • Year and Date
      2015-01-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 培養筋芽細胞を用いたオステオカルシンによる血糖調節修飾作用の検討2014

    • Author(s)
      柄慎太郎、青沼史子、細川隆司、東泉、大住伴子、平田雅人、竹内弘
    • Organizer
      第67回日本薬理学会西南部会
    • Place of Presentation
      産業医科大学(福岡県北九州市)
    • Year and Date
      2014-11-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Phosphorylation of SNAP-25 by protein kinase-A is negatively involved in calcium-dependent exocytosis in PC12 cells.2014

    • Author(s)
      Gao, J., Takeuchi, H., Wang D. and Hirata, M.
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Interaction of the C2 domains from different molecules.2014

    • Author(s)
      Wang, D., Takeuchi, H., Gao, J., Hirata, M.
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Involvement of tomosyn in insulin-dependent GLUT4 exocytosis as an Akt-substrate.2014

    • Author(s)
      Nagano, K., Takeuchi, H., Gao, J., Otani, T., Hirata, M.
    • Organizer
      39th Hormone and Cell Regulation
    • Place of Presentation
      Mont Sainte Odile, France
    • Year and Date
      2014-10-09 – 2014-10-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨格筋に対するオステオカルシンの効果について,2014

    • Author(s)
      柄慎太郎、青沼史子、細川隆司、平田雅人、竹内弘
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] オステオカルシン受容体候補タンパク質 GPRC6A の免疫組織学的解析2014

    • Author(s)
      東泉、溝上顕子、大住伴子、平田雅人、竹内弘
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] オステオカルシン経口投与時の体内動態と糖代謝への作用2014

    • Author(s)
      溝上顕子、安武雄、東泉、川久保-安河内友世、高橋一郎、竹内弘、平田雅人
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of osteocalcin on skeletal muscle cells.2014

    • Author(s)
      Tsuka, S., Aonuma, F., Hosokawa, R., Hirata, M. and Takeuchi, H.
    • Organizer
      IADR General Session
    • Place of Presentation
      Capetown, South Africa
    • Year and Date
      2014-06-25 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of regulated interaction between Akt and PRIP.2013

    • Author(s)
      竹内弘、杉山悟郎、長野公喜、大谷崇仁、高靖、平田雅人
    • Organizer
      第 86 回日本生化学会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] Akt による開口分泌調節タンパク質 tomosyn のリン酸化の検討2013

    • Author(s)
      長野公喜、竹内弘、高靖、杉山悟郎、大谷崇仁、平田雅人
    • Organizer
      第 86 回日本生化学会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] Differential role of SNAP-25 phosphorylation by protein kinase A and C in SNARE complex formation.2013

    • Author(s)
      Gao, J, Takeuchi, H., Wang, D., Hirata, M
    • Organizer
      第 86 回日本生化学会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] GLUT4 トランスロケーションにおける Akt による tomosyn のリン酸化の影響2013

    • Author(s)
      長野公喜、竹内弘、杉山悟郎、大谷崇仁、平田雅人
    • Organizer
      第 55 回歯科基礎医学会学術大会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Research-status Report
  • [Presentation] Molecular characterization of PRIP as an adaptor protein of Akt2013

    • Author(s)
      杉山悟郎、竹内弘、長野公喜、大谷崇仁、平田雅人
    • Organizer
      第 55 回歯科基礎医学会学術大会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification of tomosyn as a new downstream target of Akt.2013

    • Author(s)
      Nagano, K., Takeuchi, H., Sugiyama, G., Gao, J., Otani, T., Hirata, M.
    • Organizer
      EB2013
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Phosphorylation-dependent interaction of PRIP with Akt.2013

    • Author(s)
      ugiyama, G., Nagano, K., Otani, T., Gao, J., Takeuchi, T., Hirata, M.
    • Organizer
      EB2013
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Involvement of Phospholipase C-related but catalytically inactive protein in regulated exocytosis through the pleckstrin homology and the C2 domains.2013

    • Author(s)
      Takeuchi, H., Zhang, Z., Gao, J., Wang, DG., Hirata, M.
    • Organizer
      EB2013
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Phosphorylation of tomosyn by Akt is implicated in the regulation of GLUT4 translocation.2013

    • Author(s)
      Nagano, K. Takeuchi, H., Gao, J., Otani, T., Hirata, M.
    • Organizer
      The 8th Japan-Korea Conference on Celllular Signaling for Young Scientists
    • Place of Presentation
      福岡市
    • Related Report
      2013 Research-status Report
  • [Presentation] Negative regulation of exocytosis by PRIP through intermolecular interactions.2013

    • Author(s)
      Takeuchi, H., Zhang, Z., Gao, J., Wang, D., Hirata, M.
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of Phospholipase C-related but Catalytically Inactive Protein in Exocytosis by Direct Interaction with Syntaxin 1 and SNAP-25.2013

    • Author(s)
      Zhang, Z., Takeuchi, H., Gao, J., Wang, D., Hirata, M.
    • Organizer
      International Symposium on Human Resource Deelopment towards Global Initiative
    • Place of Presentation
      タイ王国
    • Related Report
      2012 Research-status Report
  • [Presentation] Direct interaction of phospholipase C-related but catalytically inactive protein with syntaxin 1A and SNAP25 through C2 domain.2012

    • Author(s)
      Zhang, Z., Takeuchi, H., Gao, J., Wang, D., Hirata, M.
    • Organizer
      22nd IUBMB & 37th FEBS Congress
    • Place of Presentation
      スペイン
    • Related Report
      2012 Research-status Report
  • [Presentation] Role of phosphorylation of SNAP-25 by protein kinase A on SNARE complex formation.2012

    • Author(s)
      Gao, J., Takeuchi, H., Hirata, M.
    • Organizer
      22nd IUBMB & 37th FEBS Congress
    • Place of Presentation
      スペイン
    • Related Report
      2012 Research-status Report
  • [Remarks] 所属研究室ホームページ

    • URL

      http://www.kyu-dent.ac.jp/research/lecture/oral_pharmacological

    • Related Report
      2014 Annual Research Report
  • [Remarks] 所属分野ホームページ

    • URL

      http://www.kyu-dent.ac.jp/research/lecture/oral_pharmacological

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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