Analysis of a balance between pro-inflammatory and anti-inflammatory pulpal responses in the pathogenesis of pulpitis

• Project/Area Number: 24592871
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: NAKANISHI Tadashi 徳島大学, ヘルスバイオサイエンス研究部, 准教授 (00217770)
• Co-Investigator(Kenkyū-buntansha): YUMOTO Hiromichi 徳島大学, 病院, 講師 (60284303) ; HOSOKAWA Yoshitaka 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (90346601)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯髄炎 / 歯髄細胞 / サイトカイン / 歯髄 / 炎症 / 象牙芽細胞 / リンパ球

Outline of Final Research Achievements

With advance of dental caries, the infiltration of effecter T cells and the production of various inflammatory mediators such as prostaglandin (PG) in the dental pulp tissue become apparent. In this study, we evaluated the role of a balance between pro-inflammatory and anti-inflammatory responses in the pathogenesis of pulpitis using dental pulp cells. As a result, interleukin-17 from effecter T cells induced the production of cytokines such as CXCL10 and CCL20 in the dental pulp cells. In contrast, PGF2α up-regulated CCL20 production and down-regulated CXCL10 production in the activated dental pulp cells.

Research Products

• [Journal Article] Catechins inhibit vascular endothelial growth factor production and cyclooxygenase-2 expression in human dental pulp cells. (2015)
  • Author(s): T. Nakanishi, K. Mukai, Y. Hosokawa, D. Takegawa, T. Matsuo
  • Journal Title: International Endodontic Journal Volume: 48 Issue: 3 Pages: 277-282
  • DOI: 10.1111/iej.12312
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Modulatory roles of interferon-γ through indoleamine 2,3-dioxygenase induction in innate immune response of dental pulp cells (2014)
  • Author(s): D. Takegawa, T. Nakanishi, K. Hirao, H. Yumoto, K. Takahashi, T. Matsuo
  • Journal Title: Journal of Endodontics Volume: 40 Issue: 9 Pages: 1382-1387
  • DOI: 10.1016/j.joen.2014.03.018
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 歯髄疾患の病態と診断 (2013)
  • Author(s): 松尾敬志、尾崎和美、中西 正、湯本浩通、高橋加奈子、平尾功治、武川大輔
  • Journal Title: 日本歯内療法学会雑誌 Volume: 34 Pages: 67-76
  • NAID: 10031183916
• [Presentation] Interleukin-17 regulates inflammatory mediator expression in IL-1β-stimulated dental pulp cells (2015)
  • Author(s): T. Nakanishi, D. Takegawa, K. Hirao, H. Yumoto, T. Matsuo
  • Organizer: 2015 IADR/AADR/CADR General Session and Exhibition
  • Place of Presentation: ボストン（米国）
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Interleukin-17がヒト歯髄線維芽細胞の炎症メディエーター発現に及ぼす影響 (2014)
  • Author(s): 中西 正、武川大輔、平尾功治、湯本浩通、松尾敬志
  • Organizer: 日本歯科保存学会 2014年度秋季学術大会（第141回）
  • Place of Presentation: 山形テルサ（山形県山形市）
  • Year and Date: 2014-10-30 – 2014-10-31
• [Presentation] Prostaglandin F2alpha regulates cytokine expression in dental pulp cells (2013)
  • Author(s): T. Nakanishi et al.
  • Organizer: IADR General Session & Exhibition (第91回)
  • Place of Presentation: ワシントン州コンベンションセンター（米国）
• [Presentation] Prostaglandin F2alphaは歯髄細胞における炎症メディエーター発現を調節する (2012)
  • Author(s): 中西 正 他
  • Organizer: 日本歯科保存学会 2012年度春季学術大会（第136回）
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県）