| Project/Area Number |
24592921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
MOROI RYOJI 九州大学, 大学病院, 助教 (70325471)
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| Co-Investigator(Kenkyū-buntansha) |
牧平 清超 九州大学, 歯学研究科(研究院), 准教授 (80304450)
坂井 貴子 九州大学, 大学病院, 講師 (60128022)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 骨吸収 / メカニカルストレス / RANKL / 破骨細胞 |
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| Outline of Final Research Achievements |
Mechanical loading conditions result in alteration in bone mineral density and quantity, which may be partly attributed to an imbalance in bone formation and resorption. To investigate the effect of mechanical loading (compress and stretch) on osteoblast and osteoclast differentiation, we used a stretch system to culture MC3T3-E1 and RAW264.7 cells. The expressions of Runx2, EphB4, MMP9 and TACE mRNA in MC3T3-E1 cells were enhanced under compressive force, while the expressions of Osterix, ColI and EphB4 mRNA were inhibited under stretch force. On the other hand, compressive force inhibited the enhancement of TRAP and DC-STAMP mRNA in RAW264.7 cells by RANKL. Stretch force had no effect on the enhancement of DC-STAMP mRNA expression by RANKL.
These results, taken together, demonstrate that the responses of MC3T3-E1 and RAW264.7 cells to compressive and stretch force are different.
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