A study for bone resorption of jaw at mechanical stress

• Project/Area Number: 24592921
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Kyushu University
• Principal Investigator: MOROI RYOJI 九州大学, 大学病院, 助教 (70325471)
• Co-Investigator(Kenkyū-buntansha): 牧平 清超 九州大学, 歯学研究科(研究院), 准教授 (80304450) ; 坂井 貴子 九州大学, 大学病院, 講師 (60128022)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 骨吸収 / メカニカルストレス / RANKL / 破骨細胞

Outline of Final Research Achievements

Mechanical loading conditions result in alteration in bone mineral density and quantity, which may be partly attributed to an imbalance in bone formation and resorption. To investigate the effect of mechanical loading (compress and stretch) on osteoblast and osteoclast differentiation, we used a stretch system to culture MC3T3-E1 and RAW264.7 cells. The expressions of Runx2, EphB4, MMP9 and TACE mRNA in MC3T3-E1 cells were enhanced under compressive force, while the expressions of Osterix, ColI and EphB4 mRNA were inhibited under stretch force. On the other hand, compressive force inhibited the enhancement of TRAP and DC-STAMP mRNA in RAW264.7 cells by RANKL. Stretch force had no effect on the enhancement of DC-STAMP mRNA expression by RANKL.
These results, taken together, demonstrate that the responses of MC3T3-E1 and RAW264.7 cells to compressive and stretch force are different.

Research Products

• [Journal Article] 周期性伸展刺激がRAW264.7細胞とMC3T3-E1細胞に与える影響 (2012)
  • Author(s): 牧平清超，峯裕一，首藤崇裕，寺田善博，二川浩樹
  • Journal Title: 老年歯科医学 Volume: 27 Pages: 77-86
  • NAID: 10031137201
  • Peer Reviewed
• [Presentation] 動物モデルを用いたチタン製インプラント周囲組織中のチタンに関する解析 (2014)
  • Author(s): 動物モデルを用いたチタン製インプラント周囲組織中のチタンに関する解析
  • Organizer: 日本歯科理工学会九州地方会夏期セミナー
  • Place of Presentation: 鹿児島県鹿児島市
  • Year and Date: 2014-08-08
• [Presentation] ラット実験モデルを用いたインプラント周囲粘膜炎の解析 (2014)
  • Author(s): 首藤崇裕, 和智貴紀, 片山洋子, 篠原義憲, 的野良就, 諸井亮司, 牧平清超
  • Organizer: 日本補綴歯科学会第123回学術大会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2014-05-24 – 2014-05-25
• [Presentation] インプラント体表面からの溶出チタンの検討 (2014)
  • Author(s): 和智貴紀, 首藤崇裕, 中村優介, 的野良就, 篠原義憲, 諸井亮司, 牧平清超
  • Organizer: 日本補綴歯科学会第123回学術大会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2014-05-24 – 2014-05-25
• [Presentation] チタンイオンとLPSが歯周組織に与える影響 (2013)
  • Author(s): 和智貴紀，首藤崇裕，篠原義憲, 的野良就，諸井亮司, 栗田賢一, 牧平清超
  • Organizer: 日本補綴歯科学会九州支部学術大会
  • Place of Presentation: 佐賀
• [Presentation] チタンイオン存在下でLPSが歯周組織に与える影響 (2013)
  • Author(s): 和智貴紀，首藤崇裕，篠原義憲，的野良就，諸井亮司，牧平清超
  • Organizer: 日本歯科理工学会九州地方会（夏期セミナー）
  • Place of Presentation: 長崎
• [Presentation] 周期性伸展刺激に対する破骨細胞の反応 (2012)
  • Author(s): 和智貴紀, 牧平清超，首藤崇裕，峯裕一，諸井亮司，二川浩樹，寺田善博
  • Organizer: 日本補綴歯科学会中国四国・九州支部合同学術大会
  • Place of Presentation: 広島市