The fundamental Study about regeneration of submandibular gland

• Project/Area Number: 24593011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: SHIMIZU Osamu 日本大学, 歯学部, 講師 (40260971)
• Co-Investigator(Kenkyū-buntansha): OKA Shunichi 日本大学, 歯学部, 准教授 (20256879)
• Co-Investigator(Renkei-kenkyūsha): SHIRATSUCHI Hiroshi 日本大学, 歯学部, 専修医 (50710844)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 唾液腺再生 / 顎下腺 / 細胞骨格 / integrin / duct like structure / in virto / in vitro / duct-like structure / スフェロイド培養 / 成長因子

Outline of Final Research Achievements

The aim of this study was to asses alterration in the cytoskeleton and localization of integrin during regeneration of the rat submandibular gland. The findings suggested that alpha3- and alpha6beta1-integrin regulate the regeneration and alterration in cytoskeleton effect regeneration of acinar cell from the duct like structure. Additionally, in vitro investigation of the salivary regeneration was available by this study. We can evaluate the roles of many proteins during regeneration of submandibular gland.

Research Products

• [Journal Article] Immunohistologicalstudy of small Rho GTPases and beta-catenin during regeneration of the rat submandibular gland (2012)
  • Author(s): Hiroshi Shiratsuchi, Osamu Shimizu, et al.
  • Journal Title: J Mol Hist Volume: 43 Issue: 6 Pages: 751-759
  • DOI: 10.1007/s10735-012-9437-8
  • Peer Reviewed
• [Journal Article] Alteration of the actin cytoskeleton and localisation of the alpha6beta1 and alpha3 integrins during regeneration of the rat submandibular gland (2012)
  • Author(s): Shimizu O, Shiratsuchi H, et al.
  • Journal Title: Arch Oral Biol Volume: 57 Issue: 8 Pages: 1127-1132
  • DOI: 10.1016/j.archoralbio.2012.02.018
  • Peer Reviewed
• [Presentation] ラット顎下腺再生過程におけるwnt/beta-cateninタンパク質の局在 (2015)
  • Author(s): 安光智洋、清水 治、白土博司、玉川崇浩、米原啓之
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] Immunolocalization of FGFs and FGFRs during rat submandibular gland regeneration (2015)
  • Author(s): Osamu Shimizu, Hiroshi Shiratsuchi, Tomohiro Yasumitsu, Takayuki Mashimo, Tatsuhisa Watanabe, Tadahito Saito, Yasuyuki Yonehara
  • Organizer: 93rd IADR
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] ラット顎下腺再生過程におけるFGFおよびFGFRファミリータンパク質の局在 (2013)
  • Author(s): 白土博司、清水 治、安光智洋、斎藤忠仁、真下貴之、米原啓之
  • Organizer: 第58回日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] ラット顎下腺再生過程における基礎的研究 (2012)
  • Author(s): 清水 治
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: インテックス大阪