Development of a treatment model for osteoporosis using an inner bone environment system

• Project/Area Number: 24593054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMACHIKA Eiki 岡山大学, 大学病院, 講師 (10294422)
• Co-Investigator(Kenkyū-buntansha): TSUJIGIWA Hidetsugu 岡山理科大学, 理学部, 教授 (70335628)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 骨 / 骨粗鬆症 / 間葉系幹細胞 / 骨再生

Outline of Final Research Achievements

Our 3-year research study described that (1) CD34+, c-kit+, Sca1+ mesenchymal stem cells were easy to be differentiated to bone like cells. (2) Most suitable conditions for making beta-TCP as a mesenchymal stem cells scaffold were 1200C calcination temperature and through-holes structure with diameters of 300 micro-m. (3) Results from the study of our inner bone environments system showed that T cells especially gamma-delta T cells were affected by anti-osteoporosis drug and this mechanism may be explained by the Wnt10b cell signaling.

Research Products

• [Journal Article] Hemoglobin receptor protein from Porphyromonas gingivalis induces interleukin-8 production in human gingival epithelial cells through stimulation of the mitogen-activated protein kinase and NF-κB signal transduction pathways (2014)
  • Author(s): Fujita Y, Nakayama M, Naito M, Yamachika E, Inoue T, Nakayama K, Iida S, Ohara N.
  • Journal Title: Infection and Immunity Volume: 82 Issue: 1 Pages: 202-211
  • DOI: 10.1128/iai.01140-12
  • Peer Reviewed
• [Journal Article] Effect of geometry and microstructure of honeycomb TCP scaffolds on bone regeneration (2013)
  • Author(s): Takabatake K, Yamachika E, Tsujigiwa H, Takeda Y, Kimura M, Takagi S, Nagatsuka H, Iida S
  • Journal Title: J Biomed Mater Res A. Volume: 00A Issue: 9 Pages: 1-9
  • DOI: 10.1002/jbm.a.34966
  • Peer Reviewed
• [Journal Article] Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro (2013)
  • Author(s): Tsujigiwa H, Katase N, Lefeuvre M, Yamachika E, Tamamura R, Ito S, Takebe Y, Matsuda H, Nagatsuka H
  • Journal Title: J Oral Pathol Med Volume: 42(10) Issue: 10 Pages: 799-806
  • DOI: 10.1111/jop.12080
  • Peer Reviewed
• [Journal Article] Bone regeneration from mesenchymal stem cells(MSCs) and compact bone-derived MSCs as an animal model (2013)
  • Author(s): E. Yamachika and S. Iida
  • Journal Title: Japanese Dental Science Review Volume: 49(1-2) Issue: 1 Pages: 35-44
  • DOI: 10.1016/j.jdsr.2012.11.003
  • NAID: 10031201721
  • Peer Reviewed
• [Journal Article] マウス皮質骨由来細胞による骨再生 (2013)
  • Author(s): 山近 英樹, 松原 正和, 喜多 憲一郎, 高畠 清文, 藤田 佑貴, 松村 達志, 平田 泰久, 飯田 征二
  • Journal Title: 日本口腔外科学会雑誌 Volume: 59(4) Pages: 223-229
  • NAID: 40019685876
  • Peer Reviewed
• [Journal Article] Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells (MSCs) and regeneration of bone from MSC in vivo (2012)
  • Author(s): Yamachika E, Tsujigiwa H, Matsubara M, Hirata Y, Kita K, Takabatake K, Mizukawa N, Kaneda Y, Nagatsuka H, Iida S
  • Journal Title: J Mol Histol Volume: 43(2) Issue: 2 Pages: 223-33
  • DOI: 10.1007/s10735-011-9385-8
  • Peer Reviewed
• [Presentation] リゼドロネートおよびテリパラチドのリンパ球分画へ与える影響 (2014)
  • Author(s): 山近英樹、松原正和、松井裕一、片岡陽平、藤田佑貴、石田展久、武田斉子、飯田征二
  • Organizer: 第59回日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] The effects of risedronate and teriparatide to lymphocyte ratio (2014)
  • Author(s): Eiki Yamachika, Kiyofumi Takabatake, Masakazu Matsubara, Yuuichi Matsui, Youhei Tataoka, Yuuki Fujita, Atsushi Ikeda, Nobuhisa Ishida, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Seiji Iida
  • Organizer: American association of Oral and Maxillofacial Surgens 96th annual meeting
  • Place of Presentation: Honolulu HI
  • Year and Date: 2014-09-09 – 2014-09-13
• [Presentation] ラット歯髄組織からの硬組織再生に関する基礎的研究 (2013)
  • Author(s): 山近英樹、高畠清文、松原正和、藤田佑貴、森谷徳文、山本祐也、有村友紀、池田篤司、飯田征二
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡国際会議場
• [Presentation] ハニカムβTCP細胞外微小環境下での硬組織形成 (2013)
  • Author(s): 伊藤 聡、片瀬直樹、玉村亮、武部祐一郎、山近英樹、高木愼、長塚仁
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 骨組織再建におけるハニカムTCPの形状や微細構造の影響 (2013)
  • Author(s): 高畠清文、山近英樹、飯田征二
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡国際会議場
• [Presentation] マウス骨髄由来細胞および皮質骨由来細胞による骨再生 (2012)
  • Author(s): 山近英樹、喜多憲一郎、松原正和、高畠清文、仲田直樹、石田展久、水川展吉、松村達志、高木 愼、飯田征二
  • Organizer: 第57回（社）日本口腔外科学会総会
  • Place of Presentation: 横浜
• [Presentation] OVXマウスにおけるDHNAの骨吸収抑制効果 第二報 骨量に対する統計学的解析 (2012)
  • Author(s): 喜多憲一郎、山近英樹、松原正和、石田展久、森谷徳文、松村達志、藤田佑貴、高畠清文、飯田征二
  • Organizer: 第57回（社）日本口腔外科学会総会
  • Place of Presentation: 横浜
• [Presentation] 喜多憲一郎、山近英樹、松原正和、石田展久、平田泰久、藤田佑貴、高畠清文、辻極秀次、長塚 仁、飯田征二 (2012)
  • Author(s): 骨粗鬆症モデルマウスにおける1.4-dihydroxy-2-napthoic acid(DHNA)の骨吸収抑制効果についての検討
  • Organizer: 第66回日本口腔科学会学術集会
  • Place of Presentation: 広島