The application of central analgesic activity of chemical derivatives of gamma-aminobutyric acid (GABA) to general anesthesia -the growing out of narcotic-dependent anesthesia-

• Project/Area Number: 24593058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyushu University
• Principal Investigator: ITO SHINICHI 九州大学, 歯学研究科(研究院), 助教 (00315095)
• Research Collaborator: KARUBE Noriko
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: GABA誘導体 / 全身麻酔 / シナプス前神経終末部 / 神経伝達物質 / 中枢神経系 / プレガバリン / ガバペンチン / プロポフォール / 静脈麻酔薬 / シナプス前神経終末 / 鎮痛 / 静脈内鎮静法

Outline of Final Research Achievements

We examined the sedative effects of pregabalin on the intravenous sedation (IVS) using propofol. The propofol CTs at the BIS values of 60-80 were significantly reduced in the pregabalin group. The propofol CTs were significantly lowered at Ramsay’s sedation score 3-6 in the pregabalin group. We conclude that oral premedication of pregabalin reduced the amount of propofol to obtain acceptable and adequate sedation level.
We investigated the effects of propofol on the glutamate-induced increase in [Ca2+]pre in the presynaptic boutons of hippocampal CA1 pyramidal neurons. Propofol dose-dependently suppressed glutamate-induced increase in [Ca2+]pre in boutons attached to dendrites, but not to the soma or base of the dendritic tree. Propofol may possibly have some effect on glutamate-regulated neurotransmitter release from excitatory presynaptic nerve terminals through inhibiting the increase in [Ca2+]pre induced by glutamate.

Research Products

• [Journal Article] Effects of propofol on glutamate-induced calcium mobilization in presynaptic boutons of rat hippocampal neurons (2016)
  • Author(s): Shinichi Ito, Noriko Karube, Hitomi Sugiyama, Jun Hirokawa, Seiko Kitahara, Takeshi Yokoyama
  • Journal Title: Open Journal of Anesthesiology Volume: 6 Issue: 03 Pages: 27-36
  • DOI: 10.4236/ojanes.2016.63005
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Effects of oral premedication of pregabalin on intravenous sedation using propofol. (2014)
  • Author(s): Noriko Karube, Shinichi Ito, Saori Sako, Takeshi Yokoyama.
  • Organizer: The 62nd Annual Meeting of Japanese Association for Dental Research (JADR)
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] Oral premedication of pregabalin may decrease propofol injection-induced pain. (2014)
  • Author(s): Noriko Karube, Shinichi Ito, Saori Sako, Takeshi Yokoyama.
  • Organizer: The 7th Annual Meeting of the Federation of Asian Dental Anesthesiology Societies （FADAS）
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2014-10-11
• [Presentation] Effect of Pregabalin on Intravenous Sedation Using Propofol Target-controlled Infusion. (2014)
  • Author(s): Noriko Karube, Shinichi Ito, Saori Sako, Takeshi Yokoyama.
  • Organizer: AAOMS (American Association of Oral and Maxillofacial Surgeons) 96th Annual Meeting, Scientific Sessions & Exhibition
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] 前投薬としてのプレガバリンが静脈内鎮静法に及ぼす影響 (2014)
  • Author(s): 加留部紀子, 怡土 信一, 横山 武志
  • Organizer: 第24回九州歯科麻酔シンポジウム
  • Place of Presentation: 北九州市
• [Presentation] Effects of pregabalin on intravenous sedation using propofol target-controlled infusions (2014)
  • Author(s): Noriko Karube, Shinichi Ito, Takeshi Yokoyama
  • Organizer: AAOMS 96th Annual Meeting, Scientific Sessions and Exhibition in Conjunction with the Japanese Society and Korean Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: Honolulu, HI
• [Presentation] 九州大学病院歯科麻酔科における日帰り全身麻酔症例の臨床統計的観察 (2012)
  • Author(s): 今田弘記、怡土信一、横山武志
  • Organizer: 第40回 日本歯科麻酔学会総会・学術集会
  • Place of Presentation: 福岡
• [Presentation] 中止・延期となった小児歯科日帰り全身麻酔症例の検討 (2012)
  • Author(s): 全奈穂, 坂本英治, 加藤由美子, 加留部紀子, 今田弘記, 佐古沙織, 伊藤田翔子, 表武典, 塚本真規, 怡土信一, 藤原茂樹, 横山武志
  • Organizer: 第40回 日本歯科麻酔学会総会・学術集会
  • Place of Presentation: 福岡