Cell sheets were prepared and layered to produce a hybrid cell sheet

• Project/Area Number: 24593061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: ABE Shinichi 東京歯科大学, 歯学部, 教授 (40256300)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 筋シート / 細胞シート / デスミン / ケラチン / 口腔粘膜 / 積層シート / 上皮シート

Outline of Final Research Achievements

In the present study, cell sheets were prepared using cells extracted from the oral mucosal epithelium, subepithelial tissue of the oral mucosa, and muscle tissue, and layered to produce a hybrid cell sheet. In the layered sheet, the mucosal epithelium, connective tissue layer, and muscle layer and their specific proteins increased over time with stratification. Specifically, the same structural proteins as those in vivo were expressed in the sheet.

Research Products

• [Journal Article] Expression of Cross-Linked Protein on Tissue-Engineered Epithelial Cell Sheets from Rabbit Oral Mucosa (2014)
  • Author(s): Suzuki M, Yamane S, Higa K, Umezawa T, Serikawa M, Shimazaki J, Abe S.
  • Journal Title: Journal of Hard Tissue Biology Volume: 23 Issue: 2 Pages: 275-280
  • DOI: 10.2485/jhtb.23.275
  • NAID: 130004480664
  • ISSN: 1341-7649, 1880-828X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression of cross-linked protein on tissue-engineered epithelial cell sheets from rabbit oral mucosa (2014)
  • Author(s): Suzuki, M., Yamane, S., Higa, K., Umezawa, T., Serikawa, M., Shimazaki, J. and Abe, S.
  • Journal Title: Journal of Hard Tissue Biology Volume: 23
  • NAID: 130004480664
  • Peer Reviewed
• [Journal Article] Expression of intermediate filaments in the development of genioglossus muscle. (2012)
  • Author(s): Kikuchi, A., Kishi, A., Yamamoto, M., Yamane, S., Umezawa, T., Ide, Y., Abe, S.
  • Journal Title: Journal of Hard Tissue Biology Volume: 4 Pages: 421-426
  • NAID: 130004848388
  • Peer Reviewed
• [Presentation] 骨格筋筋芽細胞シートへの間葉系細胞の影響 (2015)
  • Author(s): 梅澤貴志、山根茂樹、阿部伸一
  • Organizer: 第120回日本解剖学会総会全国学術集会
  • Place of Presentation: 栃木県
  • Year and Date: 2015-03-22
• [Presentation] 間葉系細胞の共培養が骨格筋筋芽細胞のMHCに与える影響 (2014)
  • Author(s): 梅澤貴志、山根茂樹、芹川雅光、阿部伸一
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡県
  • Year and Date: 2014-09-26
• [Presentation] 骨格筋細胞シートの解析
  • Author(s): 芹川雅光，梅澤貴志，山根茂樹，井出吉信，阿部伸一，比嘉一成，島崎 潤
  • Organizer: 東京歯科大学学会
  • Place of Presentation: 東京歯科大学（千代田区）
• [Presentation] 細胞シートとコラーゲンを用いた口腔粘膜組織の再生
  • Author(s): 下尾嘉昭，山根茂樹，比嘉一成，島崎 潤，井出吉信，阿部伸一
  • Organizer: 第293回 東京歯科大学学会例会
  • Place of Presentation: 千葉市
• [Remarks] 東京歯科大学解剖学講座
  • URL: http://www.tdc.ac.jp/dept/anat/publish.html