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Cell sheets were prepared and layered to produce a hybrid cell sheet

Research Project

Project/Area Number 24593061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionTokyo Dental College

Principal Investigator

ABE Shinichi  東京歯科大学, 歯学部, 教授 (40256300)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords筋シート / 細胞シート / デスミン / ケラチン / 口腔粘膜 / 積層シート / 上皮シート
Outline of Final Research Achievements

In the present study, cell sheets were prepared using cells extracted from the oral mucosal epithelium, subepithelial tissue of the oral mucosa, and muscle tissue, and layered to produce a hybrid cell sheet. In the layered sheet, the mucosal epithelium, connective tissue layer, and muscle layer and their specific proteins increased over time with stratification. Specifically, the same structural proteins as those in vivo were expressed in the sheet.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Expression of Cross-Linked Protein on Tissue-Engineered Epithelial Cell Sheets from Rabbit Oral Mucosa2014

    • Author(s)
      Suzuki M, Yamane S, Higa K, Umezawa T, Serikawa M, Shimazaki J, Abe S.
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 23 Issue: 2 Pages: 275-280

    • DOI

      10.2485/jhtb.23.275

    • NAID

      130004480664

    • ISSN
      1341-7649, 1880-828X
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression of cross-linked protein on tissue-engineered epithelial cell sheets from rabbit oral mucosa2014

    • Author(s)
      Suzuki, M., Yamane, S., Higa, K., Umezawa, T., Serikawa, M., Shimazaki, J. and Abe, S.
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 23

    • NAID

      130004480664

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of intermediate filaments in the development of genioglossus muscle.2012

    • Author(s)
      Kikuchi, A., Kishi, A., Yamamoto, M., Yamane, S., Umezawa, T., Ide, Y., Abe, S.
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 4 Pages: 421-426

    • NAID

      130004848388

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 骨格筋筋芽細胞シートへの間葉系細胞の影響2015

    • Author(s)
      梅澤貴志、山根茂樹、阿部伸一
    • Organizer
      第120回日本解剖学会総会全国学術集会
    • Place of Presentation
      栃木県
    • Year and Date
      2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 間葉系細胞の共培養が骨格筋筋芽細胞のMHCに与える影響2014

    • Author(s)
      梅澤貴志、山根茂樹、芹川雅光、阿部伸一
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡県
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨格筋細胞シートの解析

    • Author(s)
      芹川雅光,梅澤貴志,山根茂樹,井出吉信,阿部伸一,比嘉一成,島崎 潤
    • Organizer
      東京歯科大学学会
    • Place of Presentation
      東京歯科大学(千代田区)
    • Related Report
      2013 Research-status Report
  • [Presentation] 細胞シートとコラーゲンを用いた口腔粘膜組織の再生

    • Author(s)
      下尾嘉昭,山根茂樹,比嘉一成,島崎 潤,井出吉信,阿部伸一
    • Organizer
      第293回 東京歯科大学学会例会
    • Place of Presentation
      千葉市
    • Related Report
      2012 Research-status Report
  • [Remarks] 東京歯科大学解剖学講座

    • URL

      http://www.tdc.ac.jp/dept/anat/publish.html

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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