Development of new dental treatment stress reduction method from the brain neural network mechanism

• Project/Area Number: 24593068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tsurumi University
• Principal Investigator: KAWAHARA Hiroshi 鶴見大学, 歯学部, 教授 (10186124)
• Co-Investigator(Kenkyū-buntansha): KAWAHARA Yukie 久留米大学, 医学部, 講師 (10279135) ; ABE Keiko 鶴見大学, 歯学部, 講師 (30401334) ; HARANO Nozomu 九州歯科大学, 歯学部, 助教 (50423976)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ストレス / 神経科学 / 脳・神経 / 薬理学 / 歯学 / 精神鎮静法 / 抗不安薬 / マイクロダイアリシス

Outline of Final Research Achievements

We studied effects of the acute stress on neural activity in the rat brain.
The actions of the anxiolytis and sedatives on the central nervous system have been studied by microdialysis. This approach has shown that anxiolytis and sedatives suppress the accentuation of the locus coeruleus - medial prefrontal cortex neural activity, the accentuation of the ventral tegmental area - nucleus accumbens neuron activity, and the accentuation of the raphe - amygdala neuron activity in the stress state.

Research Products

• [Journal Article] Enhancement of analgesic effect by combination of non-noxious stimulation and noxious stimulation in humans (2015)
  • Author(s): Fujii-Abe K, Umino M, Fukayama H, Kawahara H
  • Journal Title: Pain Practice Volume: - Issue: 2 Pages: 141-147
  • DOI: 10.1111/papr.12265
  • Peer Reviewed
• [Journal Article] Effects of Lactobacillus crispatus as a candidate for oral probiotic bacteria on Haemophilus influenzae (2015)
  • Author(s): Sogabe-Ashigaki K, Imai S, Okada A, Matin K, Akimaru G, Terai T, Okamura T, Hanada N, Kawahara H
  • Journal Title: Asian Pacific Journal of Dentistry Volume: VOL15 Pages: 3-11
  • Peer Reviewed
• [Journal Article] The spontaneously hypertensive rat/Izm (SHR/Izm) shows attention deficit/hyperactivity disorder-like behaviors but without impulsive behavior: Therapeutic implications of low-dose methylphenidate (2014)
  • Author(s): Kishikawa Y, Kawahara Y, Yamada M, Kaneko F, Kawahara H, Nishi A
  • Journal Title: Behavioural Brain Research Volume: VOL274 Pages: 235-242
  • DOI: 10.1016/j.bbr.2014.08.026
  • Peer Reviewed
• [Journal Article] Food reward-sensitive interaction of ghrelin and opioid receptor pathways in mesolimbic dopamine system. (2013)
  • Author(s): Kawahara Y, Kaneko F, Yamada M, Kishikawa Y, Kawahara H, Nishi A.
  • Journal Title: Neuropharmacology Volume: 67 Pages: 395-402
  • DOI: 10.1016/j.neuropharm.2012.11.022
  • Peer Reviewed
• [Journal Article] Upregulation of the dorsal raphe nucleus-prefrontal cortex serotonin system by chronic treatment with escitalopram in hyposerotonergic Wistar-Kyoto rats. (2013)
  • Author(s): Yamada M, Kawahara Y, Kaneko F, Kishikawa Y, Sotogaku N, Poppinga WJ, Folgering JH, Dremencov E, Kawahara H, Nishi A.
  • Journal Title: Neuropharmacology Volume: 72 Pages: 169-178
  • DOI: 10.1016/j.neuropharm.2013.04.044
  • Peer Reviewed
• [Presentation] Chronic treatment with fluoxetine suppresses the stress response of serotonin by up-regulating dopamine D1 receptor signaling in the DG (2014)
  • Author(s): Kawahara Y, Shuto T, Hanada Y, Kawahara H, Nishi A
  • Organizer: 第25回マイクロダイアリシス研究会
  • Place of Presentation: お茶ノ水女子大学（東京都文京区）
  • Year and Date: 2014-12-20
• [Presentation] 注意欠陥／多動性障害様行動ラットに対する低用量メチルフェニデートの効果 (2014)
  • Author(s): 岸川由紀，河原幸江，山田麻記子，河原 博，西 昭徳
  • Organizer: 第87回日本薬理学会
  • Place of Presentation: 仙台国際センター(宮城県仙台市）
• [Presentation] 口腔顔面領域における三叉神経節ニューロンのエンドセリン受容体を介した侵害受容機構 (2013)
  • Author(s): 山本 徹，小野賢太郎，人見涼露，左合徹平，稲永清敏，渡邉誠之，河原 博
  • Organizer: 第60回日本麻酔科学会
  • Place of Presentation: ホテル札幌芸文館（北海道札幌市）
• [Presentation] グレリンの脳内オピオイドを介した食物報酬に関する中脳辺縁ドーパミン神経調節作用 (2013)
  • Author(s): 河原幸江，金子富美，山田麻記子，岸川由紀，河原 博，西 昭徳
  • Organizer: 第43回日本神経精神薬理学会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市））
• [Presentation] Yamada M, Kawahara Y, Kaneko F, Kishikawa Y, Kawahara H, Nishi A (2013)
  • Author(s): Comparison of serotonergic system in WKY and Wistar rats
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Kawahara Y, Yamada M, Kaneko F, Kishikawa Y, Kawahara H, Nishi A (2013)
  • Author(s): Food reward-sensitive interaction of ghrelin and opioid receptor pathways in mesolimbic dopamine system
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Effects of low-dose methylphenidate on attention-defict hyperactivity disorder (ADHD)-like behaviors in the spontaneously hypertensive rat, an animal model of ADHD
  • Author(s): Kishikawa Y, Kawahara Y, Yamada M ,Kawahasra H, Nishi a A
  • Organizer: 第24回マイクロダイアリシス研究会
  • Place of Presentation: お茶の水女子大学（東京都文京区）
• [Presentation] Altered responses of serotonergic system to acute and chronic escitalopram in WKY rats
  • Author(s): Yamada M, Kawahara Y, Kaneko F, Kishikawa Y, Kawahara H, Nishi A
  • Organizer: 第23回マイクロダイアリシス研究会
  • Place of Presentation: お茶の水女子大学（東京都）