Effect of bone microenvironment factors on bone healing

• Project/Area Number: 24593069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hyogo Medical University
• Principal Investigator: KAZUKI TAKAOKA 兵庫医科大学, 医学部, 講師 (60373122)
• Co-Investigator(Kenkyū-buntansha): URADE Masahiro 兵庫医科大学, 医学部, 特別招聘教授 (70104883)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨代謝 / 顎骨壊死 / TGF-β / 骨微小環境 / 破骨前駆細胞 / 骨治癒 / ビスフォスフォネート / ２型糖尿病ラット / 微小環境因子

Outline of Final Research Achievements

We evaluated the side effects of bisphosphonates on extraction socket healing in spontaneously diabetic Torii (SDT) rats, which is an established model of non-obese type 2 diabetes mellitus in study of bone microenvironment. We demonstrated the development of BRONJ-like lesions and suggested that low bone turnover with less inflammatory cell infiltration plays an important role in the development of BRONJ. The aim of this study was to determine the effects of TGF-β1 on migration of OC precursors (OPs) in the bone microenvironment. The round shape of RAW cells changed into a spindle shape by treatment with TGF-β1. The cell migration of RAW cells was decreased by treatment with TGF-β1. We showed that TGF-β1 is released from the bone matrix induces motile OPs to quiescent OPs in bone microenvironment.

Research Products

• [Presentation] 自然発症2型糖尿病ラットにおけるビスフォスフォネート関連顎骨壊死 (2014)
  • Author(s): 高岡一樹，西岡稔浩，阿部徹也，山村倫世，瀬川英美，野口一馬，浦出雅裕，岸本裕充
  • Organizer: 第59日本口腔外科学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Bisphosphonates-related osteonecrosis of the jaws using Spontaneously Diabetic Torii rats (2014)
  • Author(s): Takaoka Kazuki, Yamamura Michiyo, Nishioka Toshihiro, Abe Tetsuya, Segawa Emi, Noguchi Kazuma, Urade Masahiro, Kishimoto Hiromitsu
  • Organizer: The 2014 Annual Meeting of the American Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] Bisphosphonates-related osteonecrosis of the jaws using Spontaneously Diabetic Torii rats (2013)
  • Author(s): Kazuki Takaoka
  • Organizer: The 2nd Meeting of the International Association of Dental Research-Asia Pacific Region
  • Place of Presentation: Bangkok, Thailand
• [Presentation] 2型糖尿病ラットにおけるビスビスフォスフォネート製剤関連顎骨壊死の発症 (2012)
  • Author(s): 西岡稔浩，高岡一樹，阿部徹也，山村倫世，奥井 森，瀬川英美，大津奈央，野口一馬，岸本裕充，浦出雅裕
  • Organizer: 第66日本口腔科学会学術集会
  • Place of Presentation: 広島
• [Presentation] Bisphosphonates-related osteonecrosis of the jaws using Spontaneously Diabetic Torii rats (2012)
  • Author(s): K. Takaoka, T. Nishioka, T. Abe, M. Yamamura, N. Ohtsu, E. Segawa, Y. Zushi, S. Okui, K. Noguchi, H. Kishimoto, M. Urade,
  • Organizer: 21st Congress of the European Association for Cranio-Maxillo- Facial Surgery
  • Place of Presentation: Dubrovnik, Croatia
• [Presentation] 自然発症2型糖尿病ラットにおけるビスフォスフォネート関連顎骨壊死モデルの樹立 (2012)
  • Author(s): 高岡一樹，西岡稔浩，阿部徹也，山村倫世，奥井 森，瀬川英美，大津奈央， 野口一馬，岸本裕充，浦出雅裕
  • Organizer: 第57日本口腔外科学会学術集会
  • Place of Presentation: 東京