Elucidation of oral flora of children with no transmission of mutans streptococci

• Project/Area Number: 24593108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: TANAKA Mitsuro 岩手医科大学, 歯学部, 教授 (10143596)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ミュータンスレンサ球菌 / 感染 / 細菌叢 / 歯周病原性細菌 / T-RFLP / ストレプトコッカスミュータンス / 口腔内細菌叢 / 次世代シーケンサー / レッドコンプレックス / 口腔細菌叢

Outline of Final Research Achievements

Mutans Streptococcus (MS) was detected in 121 children out of 327. The abundance ratio was 37%. The detection ratio of P.gingivalis, T.denticola, T.forsythensis, A.actinomyceptemcomitans in 121 children who had MS in their mouth and 206 children who had no MS were 16：12、0：0、26：30、77：77 respectively. This result showed that there was no relationship between existence of the four major periodontal pathogen bacteria and existence of MS in the mouth of children.

Research Products

• [Journal Article] 小児プラークへの歯周病原性細菌の定着 (2014)
  • Author(s): 木村美澄
  • Journal Title: 岩手医科大学歯学雑誌 Volume: 38 Pages: 107-116
  • Peer Reviewed / Open Access
• [Presentation] Evaluation of T-RFLP as a tool for comprehensive analysis of oral microflora (2015)
  • Author(s): Keiko Saito, Mitsuro Tanaka
  • Organizer: 25th congress of International Association of Paediatric Dentistry
  • Place of Presentation: Glasgow, UK
  • Year and Date: 2015-07-01 – 2015-07-04
• [Presentation] 小児プラークへの歯周病原性細菌の定着 (2014)
  • Author(s): 木村美澄、下山佑、及川利佳子、松本弘紀、蒔苗剛、斎藤桂子、木村重信、田中光郎
  • Organizer: 第５２回日本小児歯科学会大会
  • Place of Presentation: 品川区立総合区民会館、東京都
  • Year and Date: 2014-05-16 – 2014-05-17
• [Presentation] 小児プラークへの歯周病原性細菌の定着 (2014)
  • Author(s): 木村美澄、下山佑、及川利佳子、松本弘紀、蒔苗剛、斎藤桂子、木村重信、田中光郎
  • Organizer: 第５２回日本小児歯科学会大会
  • Place of Presentation: 東京都品川区立総合区民会館
• [Presentation] ‘Red complex species’を含む歯周病原性細菌の小児プラークへの感染 (2013)
  • Author(s): 木村美澄、下山佑、及川利佳子、松本弘紀、木村重信、田中光郎
  • Organizer: 第５１回日本小児歯科学会学術大会
  • Place of Presentation: 岐阜市
• [Presentation] Colonization of Porphyromonas gingivalis in the plaques from mothers and their children (2012)
  • Author(s): Misumi Kimura, Yu Shimoyama, Shigenobu Kimura, Mitsuro Tanaka
  • Organizer: 11th congress of the European Academy of Pediatric Dentistry
  • Place of Presentation: Strasbourg, France
• [Presentation] 小児プラークへのPorphyromonas gingivalisの感染 (2012)
  • Author(s): 木村美澄，下山佑，及川利佳子，田中光郎
  • Organizer: 第66回日本細菌学会東北支部総会
  • Place of Presentation: 仙台