| Project/Area Number |
24593117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
IWASAKI Kengo 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (40401351)
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| Co-Investigator(Kenkyū-buntansha) |
KOMAKI Motohiro 東京医科歯科大学, 医歯(薬)学総合研究科, 寄付講座准教授 (30401368)
MORITA Ikuo 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60100129)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯周病 / 再生 / 幹細胞 / 歯周組織 |
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| Outline of Final Research Achievements |
Periodontitis is chronic inflammatory disease, which is characterized by the destruction of the tooth supporting tissues. In this study, we investigated our hypothesis that transplantation of paracrine factors from periodontal ligament stem cells (PDLSC) could induce the regeneration of lost periodontal tissues. We transplanted 3 different paracrine factor preparations (Control-CM, PDLSC-CM and Fibroblast-CM) into surgically created periodontal defects in rats. After 4 weeks of transplantation, PDLSC-CM demonstrated significant new bone formation under micro-CT observations compared with other 2 groups. It was also revealed that PDLSC-CM contained various growth factors, cytokines and anigiogenic factors in protein array analysis. Moreover, we found that gene expression of TNF-alpha was reduced by the PDLSC-CM transplantation in regenerating rat periodontal tissue, suggesting the relationships between reduction of inflammation and tissue regeneration.
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