Nobel periodontal regenerative therapy using paracrine factors from stem cells

• Project/Area Number: 24593117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IWASAKI Kengo 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (40401351)
• Co-Investigator(Kenkyū-buntansha): KOMAKI Motohiro 東京医科歯科大学, 医歯(薬)学総合研究科, 寄付講座准教授 (30401368) ; MORITA Ikuo 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60100129)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯周病 / 再生 / 幹細胞 / 歯周組織

Outline of Final Research Achievements

Periodontitis is chronic inflammatory disease, which is characterized by the destruction of the tooth supporting tissues. In this study, we investigated our hypothesis that transplantation of paracrine factors from periodontal ligament stem cells (PDLSC) could induce the regeneration of lost periodontal tissues. We transplanted 3 different paracrine factor preparations (Control-CM, PDLSC-CM and Fibroblast-CM) into surgically created periodontal defects in rats. After 4 weeks of transplantation, PDLSC-CM demonstrated significant new bone formation under micro-CT observations compared with other 2 groups. It was also revealed that PDLSC-CM contained various growth factors, cytokines and anigiogenic factors in protein array analysis. Moreover, we found that gene expression of TNF-alpha was reduced by the PDLSC-CM transplantation in regenerating rat periodontal tissue, suggesting the relationships between reduction of inflammation and tissue regeneration.

Research Products

• [Journal Article] Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion. (2014)
  • Author(s): Iwasaki K, Komaki M, Yokoyama N, Tanaka Y, Taki A, Honda I, Kimura Y, Takeda M, Akazawa K, Oda S, Izumi Y, Morita I.
  • Journal Title: Tissue Eng Part A Volume: 20 Pages: 693-704
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion. (2014)
  • Author(s): Iwasaki K, Komaki M, Yokoyama N, Tanaka Y, Taki A, Honda I, Kimura Y, Takeda M, Akazawa K, Oda S, Izumi Y, Morita I.
  • Journal Title: Tissue Eng Part A. Volume: 20 Pages: 693-704
  • DOI: 10.1089/ten.tea.2013.0017
  • Peer Reviewed
• [Presentation] 歯根膜幹細胞由来液性因子を用いた歯周組織再生 (2013)
  • Author(s): 岩崎剣吾, 小牧基浩, 赤澤惠子, 横山尚毅, 菖蒲弘人, 永田瑞, 木村康之, 遠井政行, 和泉雄一, 森田育男
  • Organizer: 第56回秋季日本歯周病学会学術大会
  • Place of Presentation: 前橋
• [Presentation] 歯根膜幹細胞培養上清を用いたラット歯周組織の再生 (2013)
  • Author(s): 岩崎剣吾, 小牧基浩 ,横山尚毅, 菖蒲弘人,森田育男
  • Organizer: 第34回日本炎症再生医学会
  • Place of Presentation: 京都
• [Presentation] 歯根膜幹細胞の培養過程における形態および分化能の変化について (2013)
  • Author(s): 岩崎剣吾, 小牧基浩, 赤澤惠子, 横山尚毅, 菖蒲弘人,木村康之, 遠井政行, 和泉雄一, 森田育男
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: 東京
• [Presentation] ラット臼歯部歯周組織欠損に対する歯根膜幹細胞転写羊膜を用いた歯周組織再生治療について (2012)
  • Author(s): 岩崎剣吾, 小牧基浩, 木村康之, 横山尚毅, 和泉雄一, 森田育男
  • Organizer: 第55回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌
• [Presentation] 歯根膜幹細胞転写羊膜移植によるラット歯周組織の再生 (2012)
  • Author(s): 岩崎 剣吾，小牧 基浩，滝 敦子，本多 泉，森田 育男
  • Organizer: 第33回日本炎症再生医学会
  • Place of Presentation: 福岡