Usefulness of the complex consisting of three regenerative factors, which can be processed and shaped in vitro for regeneration of severely destructed periodontal tissues

• Project/Area Number: 24593122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Hiroshima University
• Principal Investigator: SHIBA Hideki 広島大学, 医歯薬保健学研究院(歯), 教授 (60260668)
• Co-Investigator(Kenkyū-buntansha): FUJITA Tsuyoshi 広島大学, 病院, 講師 (80379883) ; TAKEDA Katsuhiro 広島大学, 大学院医歯薬保健学研究院, 助教 (10452591)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周療法学 / 歯周組織再生 / 幹細胞 / 細胞外基質 / LL37 / 再生3要素 / 歯周外科学 / 再生３要素

Outline of Final Research Achievements

We developed the ball-shaped mesenchymal stem cell complex (B-MSC) which contains bone marrow-derived MSC, self-produced extracellular matrices and no artificial scaffold, and which can be processed in vitro. Furthermore, the osteogenic-differentiated B-MSC and the synthesized LL37 which increases the production of vascular endothelial growth factor enhanced bone regeneration in the rat calvarial defect model. Therefore, the transplantation of B-MSC and LL37 may represent a novel tissue engineering therapy for regeneration of periodontal tissues (bone).

Research Products

• [Journal Article] Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration. (2015)
  • Author(s): Kittaka M, Kajiya M, Shiba H, Takewaki M, Takeshita K, Khung R, Fujita T, Iwata T, Nguyen TQ, Ouhara K, Takeda K, Fujita T, Kurihara H.
  • Journal Title: Cytotherapy. Volume: 2 Issue: 7 Pages: 48-61
  • DOI: 10.1016/j.jcyt.2015.01.007
  • Peer Reviewed / Open Access
• [Journal Article] The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect (2013)
  • Author(s): Kittaka M., Shiba H., Kajiya M., Fuita T., Iwata T., Rathvisa K., Ouhara K., Takeda K., Fujita T., Komatsuzawa H., Kurihara, H.
  • Journal Title: Peptides Volume: 46 Pages: 136-42
  • DOI: 10.1016/j.peptides.2013.06.001
  • Peer Reviewed
• [Journal Article] Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells. (2013)
  • Author(s): Kittaka M
  • Journal Title: J Periodontal Res Volume: Apr;48(2) Issue: 2 Pages: 228-34
  • DOI: 10.1111/j.1600-0765.2012.01524.x
  • Peer Reviewed
• [Presentation] 骨髄間葉系幹細胞集塊Clumps of a MSC/ECM complex(C-MSC)を用いた新規組織再生治療法の開発 (2014)
  • Author(s): 加治屋幹人、柴秀樹、竹脇学、竹下慶、Khung Rathvisal、Nguyen Quoc Troung、岩田倫幸、應原一久、武田克浩、藤田剛、栗原英見
  • Organizer: 日本歯科医学会 第30回「歯科医学を中心とした総合的な研究を推進する集い」
• [Presentation] 骨髄間葉系幹細胞集塊Clumps of mesenchymal stem cells/extracellular matrix complexを利用した新規組織再生療法開発 (2014)
  • Author(s): 加治屋幹人、 橘高瑞穂、 柴秀樹、 竹脇学、 竹下慶、 岩田倫幸、應原一久、武田克浩、藤田剛、栗原英見
  • Organizer: 第57回秋季日本歯周病学会学術大会
• [Presentation] 抗菌ペプチドLL37はラット頭蓋骨骨欠損モデルにおける骨再生を促進する (2012)
  • Author(s): 橘髙瑞穂, 藤田 剛, 柴 秀樹, 栗原英見
  • Organizer: 第54回歯科基礎医学会学術大会ならび総会
  • Place of Presentation: 郡山市