An experimental study of mechanism of periodontal and peri-implant tissue destruction.

• Project/Area Number: 24593127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Nagasaki University
• Principal Investigator: HARA Yoshitaka 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (60159100)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Atsutoshi 長崎大学, 大学院医歯薬学総合研究科(歯学系), 准教授 (70253680) ; UKAI Takashi 長崎大学, 病院(歯学系), 講師 (20295091) ; KANEKO Takashi 福岡歯科大学, 歯学部, 教授 (10284697) ; YOSHINAGA Yasunori 福岡歯科大学, 歯学部, 准教授 (60452869)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: インプラント周囲炎 / 歯周炎 / 免疫複合体 / 骨吸収 / LPS / ラット / 病理組織学的研究 / インプラント / 実験病理学的研究 / 歯周ポケット / A. actinomycetemcomitans / S. aureus / 免疫組織学的研究

Outline of Final Research Achievements

We applied our experimental periodontitis model to the peri-implant tissues and compared periimplant destruction to periodontitis without using a ligature.
Rats were divided into five groups. Implantation was performed after extraction of right first molars in rats. The left first molars were left untreated to be examined as natural teeth. The immunized group consisted of rats that had received intraperitoneal LPS, whereas the non immunized group received only PBS. The untreated baseline group received only implantation. After booster injection, half of each group received topical application of LPS in the palatal gingival sulcus daily for 3 days. The other half of the groups received PBS. Histopathological and histometrical findings were observed. Peri-implant tissue destruction was greater in the immunized and LPS-applied groups than in the other groups. No periodontal destruction was observed. Antigen-induced peri-implant tissue destruction occurs faster than periodontal tissue

Research Products

• [Journal Article] Histopathological comparison of the onset of peri-implantitis and periodontitis in rats (2016)
  • Author(s): Yuzo Takamori, Ikiru Atsuta, Hirotaka Nakamura, Takashi Sawase, Kiyoshi Koyano, Yoshitaka Hara
  • Journal Title: Clinical Oral Implant Research Volume: 27 Pages: 1-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gram-positive bacteria as an antigen topically applied into gingival sulcus of immunized rat accelerates periodontal destruction (2013)
  • Author(s): Fumiko Nagano , Takashi Kaneko, Yasunori Yoshinaga, Takashi Ukai, Akiko Kuramoto, Susumu Nakatsu, Kazushi Oshino, Ikuhisa Ichimura, Yoshitaka Hara
  • Journal Title: Journal of Periodontal Research Volume: 48
  • Peer Reviewed
• [Presentation] ラット実験的歯周炎モデルを用いた歯周炎とインプラント周囲炎の病理組織学的比較 (2015)
  • Author(s): 髙森雄三, 中村弘隆, 藏本明子, 吉村篤利, 鵜飼 孝, 尾崎幸生, 白石千秋, 山下恭德, 泉 聡史, 小林弘樹, 小山美香, 原 宜興
  • Organizer: 日本歯科保存学会2015年度秋季学術大会
  • Place of Presentation: 文京シビックホール（東京）
  • Year and Date: 2015-11-12
• [Presentation] Histopathological study of peri-implantitis and periodontitis in rat (2015)
  • Author(s): Yuzo Takamori, et al
  • Organizer: General meeting of Asian-Pacific Society of Periodontology
  • Place of Presentation: Bali (Indonesia)
  • Year and Date: 2015-10-08 – 2015-10-09
• [Presentation] Histopathological Comparison Of Destruction Between Peri-implant And Periodontal Tissue In Rats. (2015)
  • Author(s): Takamori Y, Takamori A, Yoshinaga Y, Ukai T, Ozaki Y, Shiraishi C, Izumi S, Kobayashi H, Nakamura H, Yoshimura A, Hara Y
  • Organizer: 11th Asian Pacific Society of Periodontology meeting
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2015-10-07
  • Int'l Joint Research
• [Presentation] ラットにおけるグラム陽性細菌の感作および歯肉溝内滴下は歯周組織破壊を促進する (2013)
  • Author(s): 長野史子、金子高士、吉永泰周、鵜飼孝、藏本明子、中津晋、高森雄三、野口恵司、原 宜興
  • Organizer: 第４回口腔環境制御研究カテゴリー集会
  • Place of Presentation: 長崎
• [Presentation] 菌体破砕物による感作とその歯肉溝内滴下は歯周ポケットの形成を誘導する (2012)
  • Author(s): 長野史子、吉永泰周、金子高士、藏本明子、中津晋、高森雄三、野口恵司、岸本隆明、原 宜興
  • Organizer: 第５４回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌
• [Presentation] Topical application of sonicated and heat-treated Staphyrococcus aureus or Aggregatebacter actinomycetemcomitans into rat gingival sulcus induces periodontal tissue destruction after immunization. (2012)
  • Author(s): Fumiko Nagano, Yasunori Yoshinaga, Takashi Kaneko, Akiko Kuramoto, Susumu Nakatsu, Takaaki Kishimoto, Yuzo Takamori, Satosi Noguchi, Yoshitaka Hara
  • Organizer: 7th Conference of the Eyropean Federation of Periodontology
  • Place of Presentation: Vienna