The role of IL-35 on Th17 cells in inflamed gingiva

• Project/Area Number: 24593134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: MITANI Akio 愛知学院大学, 歯学部, 教授 (50329611)
• Co-Investigator(Kenkyū-buntansha): KIKUCHI Takeshi 愛知学院大学, 歯学部, 講師 (40421242) ; AINO Makoto 愛知学院大学, 歯学部, 助教 (20572811) ; ISHIHARA Yuichi 松本歯科大学, 歯学部, 教授 (50261011)
• Research Collaborator: FUJIMURA Takeki 愛知学院大学, 歯学部, 助教 (40749892)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: IL-35 / 歯周組織 / Th17 / 歯学 / 免疫学 / IL-17 / 歯周炎

Outline of Final Research Achievements

IL-17- and IL-35-related gene expressions in gingiva and that protein levels in GCF were higher in patients with chronic periodontitis compared to the healthy group. There was a tendency toward a negative correlation between IL-17 and IL-35 in GCF samples; however, this was not significant. Furthermore, rIL-35 inhibited the Th17-related genes expression and IL-17 protein production in Th17 cells. These results suggest that IL-35 may directly act to the Th17 cells, and followed by inhibition of the IL-17 production.
Taken together, IL-35 might play a suppressive role in periodontal disease by inhibition of IL-17 production from Th17 cells in order to suppress excessive inflammation in gingiva.

Research Products

• [Journal Article] Increased expression of interleukin (IL)-35 and IL-17, but not IL-27, in gingival tissues with chronic periodontitis (2015)
  • Author(s): Mitani A, Niedbala W, Fujimura T, Mogi M, Miyamae S, Higuchi N, Abe A, Hishikawa T, Mizutani M, Ishihara Y, Nakamura H, Kurita K, Ohno N, Tanaka Y, Hattori M, Noguchi T
  • Journal Title: J Periodontol Volume: 86 Issue: 2 Pages: 301-309
  • DOI: 10.1902/jop.2014.140293
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Irradiation with a low-level diode laser induces the developmental endothelial locus-1 gene and reduces pro-inflammatory cytokines in epithelial cells (2014)
  • Author(s): Fujimura T, Mitani A, Fukuda M, Mogi M, Osawa K, Takahashi S, Aino M, Iwamura Y, Miyajima S, Yamamoto H, Noguchi T
  • Journal Title: Lasers Med Sci Volume: 29 Issue: 3 Pages: 987-994
  • DOI: 10.1007/s10103-013-1439-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inflammatory bone loss in experimental periodontitis induced by Aggregatibacter actinomycetemcomitans in IL-1Ra knockout mice. (2014)
  • Author(s): Izawa A, Ishihara Y, Mizutani H, Kobayashi S, Goto H, Okabe E, Takeda H, Ozawa Y, Kamiya Y, Sugita Y, Kubo K, Kamei H, Kikuchi T, Mitani A, Hayashi J, Nishihara T, Maeda H, Noguchi T.
  • Journal Title: Infect Immun Volume: 82 Issue: 5 Pages: 1904-1913
  • DOI: 10.1128/iai.01618-13
  • Peer Reviewed
• [Journal Article] Effect of IL-15 and natural killer cells on osteoclast and osteoblast in a mouse coculture (2014)
  • Author(s): Takeda H, Kikuchi T, Soboku K, Okabe I, Mizutani H, Mitani A, Ishihara Y, Noguchi T
  • Journal Title: Inflammation Volume: 37 Issue: 3 Pages: 657-669
  • DOI: 10.1007/s10753-013-9782-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Periodontitis-activated monocytes/macrophages cause aortic inflammation (2014)
  • Author(s): Miyajima S, Naruse K, Kobayashi Y, Nakamura N, Nishikawa T, Adachi K, Suzuki Y, Kikuchi T, Mitani A, Mizutani M, Ohno N, Noguchi T, Matsubara T
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 5171-5171
  • DOI: 10.1038/srep05171
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Morphological Alterations of Periodontal Pocket Epithelium Following Nd:YAG Laser Irradiation (2014)
  • Author(s): Ting CC, Fukuda M, Watanabe T, Sanaoka A, Mitani A, Noguchi T
  • Journal Title: Photomed Laser Surg Volume: 32 Issue: 12 Pages: 649-657
  • DOI: 10.1089/pho.2014.3793
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Relationship between findings of mandibular cortical bone in inferior border and bone mineral densities of lumbar vertebrae in postmenopausal women (2014)
  • Author(s): Naitoh M, Takada ST, Kurosu Y, Inagaki K, Mitani A, Ariji E
  • Journal Title: Okajimas Folia Anatomica Japonica Volume: 91 Issue: 3 Pages: 49-55
  • DOI: 10.2535/ofaj.91.49
  • NAID: 130004773615
  • ISSN: 0030-154X, 1881-1736
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Five-year clinical results for treatment of intrabony defects with EMD, guided tissue regeneration and open-flap debridement: a case series (2014)
  • Author(s): Mitani A,Takasu H,Horibe T, Furuta H, Nagasaka T, Aino M, Fukuda M, Fujimura T, Mogi M, Noguchi T.
  • Journal Title: J Periodont Res Volume: 印刷中 Issue: 1 Pages: 123-130
  • DOI: 10.1111/jre.12188
  • Peer Reviewed / Open Access
• [Journal Article] Management of traumatic dental injury after periodontal surgery in patient with hereditary gingival fibromatosis: Case report (2014)
  • Author(s): Inagaki K, Kamei H, Mitani A, Noguchi T.
  • Journal Title: Oral Health Dent Manag Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Follicular dendritic cell-secreted protein expression is reduced in experimental periodontitis concurrently with the up-regulation of IL-17 mRNA and an increase in the RANKL/OPG mRNA ratio (2013)
  • Author(s): Takahashi S, Fukuda M, Mitani A, Fujimura T, Iwamura Y, Sato S, Kubo T, Sugita Y, Maeda H, Shinomura T, Noguchi T.
  • Journal Title: J Periodont Res Volume: 印刷中 Issue: 3 Pages: 390-397
  • DOI: 10.1111/jre.12118
  • Peer Reviewed / Open Access
• [Journal Article] Nitric Oxide-induced regulatory T cells inhibit Th17 but not Th1 cell differentiation and function (2013)
  • Author(s): Niedbala W, Besnard AG, Jiang HR, Alves-Filho JC, Fukada SY, Nascimento D, Mitani A, Pushparaj P, Alqahtani MH, Liew FY.
  • Journal Title: J Immunol Volume: 191 Issue: 1 Pages: 164-170
  • DOI: 10.4049/jimmunol.1202580
  • Peer Reviewed
• [Journal Article] Antimicrobial photodynamic therapy using a diode laser with a potential new photosensitizer, indocyanine green-loaded nanospheres, may be effective for the clearance of Porphyromonas gingivalis (2013)
  • Author(s): Nagahara A, Mitani A, Fukuda M, Yamamoto H, Tahara K, Morita I, Ting CC, Watanabe T, Fujimura T, Osawa K, Sato S, Takahashi S, Iwamura Y, Kuroyanagi T, Kawashima Y, Noguchi T.
  • Journal Title: J Periodontal Res Volume: 48 Issue: 5 Pages: 591-599
  • DOI: 10.1111/jre.12042
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide of Aggregatibacter actinomycetemcomitans up-regulates inflammatory cytokines, prostaglandin E2 synthesis and osteoclast formation in interleukin-1 receptor antagonist-deficient mice (2013)
  • Author(s): Mizutani H, Ishihara Y, Izawa A, Fujihara Y, Kobayashi S, Gotou H, Okabe E, Takeda H, Ozawa Y, Kamiya Y, Kamei H, Kikuchi T, Yamamoto G, Mitani A, Nishihara T, Noguchi T.
  • Journal Title: J Periodont Res Volume: 48 Issue: 6 Pages: 748-756
  • DOI: 10.1111/jre.12065
  • Peer Reviewed
• [Journal Article] Aggregatibacter actinomycetemcomitans lipopolysaccharide stimulated epithelial cells produce interleukin-15 that regulates T cell activation (2013)
  • Author(s): Suga T, Mitani A, Mogi M, Kikuchi T, Fujimura T, Takeda H, Hishikawa T, Yamamoto G, Hayashi J, Ishihara Y, Noguchi T.
  • Journal Title: Arch Oral Biol Volume: 58 Issue: 10 Pages: 1541-1548
  • DOI: 10.1016/j.archoralbio.2013.06.020
  • Peer Reviewed
• [Journal Article] Nd:YAG laser irradiation of the tooth root surface inhibits demineralization and root surface softening caused by minocycline application (2013)
  • Author(s): Watanabe T, Fukuda M, Mitani A, Ting CC, Osawa K, Nagahara A, Sato S, Fujimura T, Takahashi S, Iwamura Y, Murakami T, Noguchi T.
  • Journal Title: Photomed Laser Surg Volume: 31 Issue: 12 Pages: 571-577
  • DOI: 10.1089/pho.2013.3561
  • Peer Reviewed
• [Journal Article] Irradiation with a low-level diode laser induces the developmental endothelial locus-1 gene and reduces pro-inflammatory cytokines in epithelial cells (2013)
  • Author(s): Fujimura T, Mitani A, Fukuda M, Mogi M, Osawa K, Takahashi S, Aino M, Iwamura Y, Miyajima S, Yamamoto H, Noguchi T.
  • Journal Title: Lasers Med Sci Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Effect of IL-15 and natural killer cells on osteoclast and osteoblast in a mouse coculture (2013)
  • Author(s): Takeda H, Kikuchi T, Soboku K, Okabe I, Mizutani H, Mitani A, Ishihara Y, Noguchi T.
  • Journal Title: Inflammation Volume: 印刷中
  • Peer Reviewed
• [Journal Article] 日本人における歯周病指数と心臓血管疾患との関連について ― 愛知県豊橋市の健診結果 ― (2012)
  • Author(s): 三谷章雄，大澤数洋，森田一三，林潤一郎，伊藤正満，匹田雅久，佐藤聡太，川瀬仁史，高橋伸行，武田紘明，藤村岳樹，福田光男，前田初彦，野口俊英ほか
  • Journal Title: 日歯保存誌 Volume: 55巻5号 Pages: 313-319
  • NAID: 110009596713
  • Peer Reviewed
• [Presentation] Surface modification of polyvinylidene chloride films using argon ion bombardment for guided bone regeneration (2015)
  • Author(s): Kobayashi S, Hayashi T, Okabe E, Kawase M, Suga T, Noguchi T, Kawai T, Mitani A
  • Organizer: 2015 IADR/AADR/CADR General Session & Exhibition
  • Place of Presentation: Boston
  • Year and Date: 2015-03-13
• [Presentation] Effects of mouthrinse on gingivitis and bacterial flora (2014)
  • Author(s): Tobata H, Kitamura M, Inagaki K, Fujita T, Maeda K, Miki K, Tauchi T, Ozasa M, Mizutani H, Fujimura T, Kawaguchi H, Mizuno N, Ohara K, Hayashi Y, Inoue M, Kurihara H, Mitani A, Noguchi T, Murakami S
  • Organizer: The 62th Annual Meeting of International Association for Dental Research Japanese Division
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-05
• [Presentation] 歯周炎により活性化された単核球が大動脈の炎症を惹起する機序の検討 (2014)
  • Author(s): 宮島真一，成瀬桂子，中村信久，西川徹，足立圭，鈴木佑基，水谷誠，菊池毅，野口俊英，大野紀和，松原達昭，三谷章雄
  • Organizer: 第57回秋季日本歯周病学会
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-19
• [Presentation] Interleukin-1 receptor antagonistは歯肉上皮細胞からのMatrix metalo- peptidase 13発現を抑制する (2014)
  • Author(s): 後藤久嗣, 石原裕一, 伊澤有郎, 岡部栄治郎, 久保勝俊, 杉田好彦, 前田初彦, 野口俊英, 三谷章雄
  • Organizer: 第57回秋季日本歯周病学会
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-19
• [Presentation] GIPは歯周炎を抑制する (2014)
  • Author(s): 鈴木佑基、成瀬桂子、小林泰子、中村信久、西川徹、宮島真一、足立圭、水谷誠、菊池毅、宮澤健, 月山克史, 山田祐一郎, 後藤滋巳, 大野紀和、三谷章雄、松原達昭
  • Organizer: 第回日本糖尿病合併症学会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-03
• [Presentation] High levels of sIL-1RAcP was observed in chronic periodontitis patients (2014)
  • Author(s): Yosuke Kamiya, Yuichi Ishihara, Hidehiko Kamei, Ario Izawa, Eijiro Okabe, Hisashi Goto, Akane Kuno, Yuji Sugawa, Yusuke Ozawa, Akio Mitani
  • Organizer: 2014 Annual Meeting of American Academy of Periodontology
  • Place of Presentation: San Francisco
  • Year and Date: 2014-09-20
• [Presentation] ヒト歯槽骨由来未分化骨芽細胞の骨原性維持に関わるマーカーの探索 (2014)
  • Author(s): 齋藤正寛, 相野 誠, 三谷章雄
  • Organizer: 第140回春季日本歯科保存学会
  • Place of Presentation: 大津
  • Year and Date: 2014-06-19
• [Presentation] T-RFLP法による咽頭領域の細菌叢解析と口臭との関連 (2014)
  • Author(s): 岩村侑樹, 福田光男, 三谷章雄, 林潤一郎, 佐藤聡太, 高橋伸行, 藤村岳樹, 岡田康佑, 村上多惠子, 佐藤孝至, 嶋﨑義浩, 野口俊英
  • Organizer: 第57回春季日本歯周病学会
  • Place of Presentation: 岐阜
  • Year and Date: 2014-05-23
• [Presentation] GIPは歯周炎を抑制する (2014)
  • Author(s): 鈴木佑基, 成瀬桂子, 小林泰子, 中村信久, 西川徹, 宮島真一, 足立圭, 水谷誠, 菊池毅, 宮澤健, 月山克史, 山田祐一郎, 後藤滋巳, 大野紀和, 三谷章雄, 松原達昭
  • Organizer: 第57回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22
• [Presentation] 歯科用コーンビームCTにおける歯根膜腔観察を目的とした最適構成フィルタの検討 (2013)
  • Author(s): 法野祐規、菱川敏光，後藤賢一、内藤宗孝、中山美和、吉田和史、三谷章雄、野口俊英、有地榮一郎、小寺吉衛.
  • Organizer: 日本歯科放射線学会
  • Place of Presentation: 福岡
• [Presentation] 垂直性骨欠損に対し GTR 法とエナメルマトリックスタンパク質を用いた歯周組織再生療法を行った症例の 5 年経過時における臨床的比較 (2013)
  • Author(s): 相野誠, 三谷章雄, 高須英成, 古田英豊, 永坂太郎, 三輪晃資, 菊池毅, 川瀬仁史, 伊藤正満, 林潤一郎, 黒須康成,石原裕一, 稲垣幸司, 福田光男, 野口俊英.
  • Organizer: 日本歯科保存学会春期学術大会
  • Place of Presentation: 福岡
• [Presentation] ICGナノ粒子による軟組織透過エネルギー吸収の基礎的検討 (2013)
  • Author(s): 岡田康佑, 福田光男, 三谷章雄, 山本浩充, 西田英作, 佐藤聡太, 高橋伸行, 藤村岳樹, 岩村侑樹, 須賀貴行, 渡辺智久, 佐々公太, 大澤数洋, 永原絢子, 河合達志, 野口俊英.
  • Organizer: レーザー歯学会
  • Place of Presentation: 東京
• [Presentation] ポケット外照射を想定したインドシアニングリーン封入ナノ粒子が半導体レーザー照射によるエネルギー吸収・温度変化に与える影響 (2013)
  • Author(s): 岩村侑樹, 福田光男, 三谷章雄, 山本浩充, 西田英作, 佐藤聡太, 高橋伸行, 藤村岳樹, 岡田康佑, 河合達志, 野口俊英.
  • Organizer: 愛知学院大学歯学会
  • Place of Presentation: 名古屋
• [Presentation] 歯周病における Follicular Dendritic Cell-Secreted Protein(FDC-SP)の接合上皮での発現について
  • Author(s): 高橋伸行, 福田光男, 三谷章雄, 佐藤聡太, 藤村岳樹, 渡辺智久, 永原絢子, 大澤数洋, 前田初彦, 久保勝俊, 杉田好彦, 篠村多摩之, 大城暁子, 野口俊英
  • Organizer: 第 55 回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌
• [Presentation] 歯周炎患者における末梢血血清中の sIL-1RAcP と臨床所見の関係
  • Author(s): 水谷大樹, 石原裕一, 伊澤有郎, 小澤佑介, 相野 誠, 亀井英彦, 三谷章雄, 林潤一郎, 稲垣幸司, 福田光男, 野口俊英
  • Organizer: 第 55 回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌
• [Presentation] IL-15 の炎症性骨代謝に対する影響
  • Author(s): 武田紘明, 菊池 毅, 惣ト響子, 相野 誠, 三谷章雄, 石原裕一, 野口俊英
  • Organizer: 第 55 回春季日本歯周病学会学術大会
  • Place of Presentation: 札幌
• [Presentation] IL-15の炎症性骨代謝に対する影響
  • Author(s): 武田紘明, 菊池 毅, 惣ト響子, 藤田幸子, 三谷章雄, 相野誠, 藤村岳樹, 野口俊英
  • Organizer: 第80回愛知学院大学歯学会
  • Place of Presentation: 名古屋
• [Presentation] 骨縁下欠損に対するエナメルマトリックスタンパク質を応用した歯周組織再生療法５年経過症例の臨床的検討
  • Author(s): 三谷章雄，高須英成，吉成伸夫，伊藤正満，川瀬仁史，相野誠，林潤一郎，稲垣幸司，黒須康成，三輪晃資，石原裕一，福田光男，野口俊英
  • Organizer: 第136回日本歯科保存学会春季学術大会
  • Place of Presentation: 沖縄
• [Presentation] The Effect of Interleukin-15 on Inflammatory Bone Metabolism
  • Author(s): Akio MITANI, Hiroaki TAKEDA, Takeshi KIKUCHI, Kyoko SOBOKU, Sachiko FUJITA, Yuichi ISHIHARA, Toshihide NOGUCHI
  • Organizer: EUROPEAN CONGRESS OF IMMUNOLOGY
  • Place of Presentation: Glasgow, Scotland, UK
• [Presentation] 半導体レーザーと光感受性物質を用いたaPDTが上皮細胞に与える影響
  • Author(s): 藤村岳樹、福田光男、三谷章雄、永原絢子、山本浩充、大澤数洋、佐藤聡太、高橋伸行、岩村侑樹、須賀貴行、渡辺智久、佐々公太、林潤一郎、菊池毅、別所優、野口俊英
  • Organizer: 第55回日本歯周病学会秋季学術大会
  • Place of Presentation: 筑波
• [Presentation] 遠赤外線分光法を用いたクレンチング時脳血流量の日間変動測定
  • Author(s): 菱川敏光、三谷章雄、亀井英彦、杉浦進介、伊澤有郎、藤村岳樹、足立圭、石田直之、後藤長樹、野口俊英
  • Organizer: 第55回日本歯周病学会秋季学術大会
  • Place of Presentation: 筑波
• [Presentation] Serum sIL-1RAcP levels are correlated with periodontal disease severity
  • Author(s): Y Ishihara, H Mizutani, A Izawa, E Okabe, H Goto, Y Ozawa, M Aino, H Kamei, A Mitani, J Hayashi, K Inagaki, M Fukuda, T Noguchi
  • Organizer: 98TH Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles, USA
• [Presentation] 半導体レーザーの軟組織透過性に関する基礎的検討
  • Author(s): 岩村侑樹、福田光男、三谷章雄、大澤数洋、永原絢子、山本浩充、佐藤聡太、高橋伸行、藤村岳樹、須賀貴行、渡辺智久、佐々公太、野口俊英
  • Organizer: 第24回日本レーザー歯学会総会・学術大会
  • Place of Presentation: 神戸
• [Presentation] 歯肉接合上皮(ラット)におけるFollicular Dendritic Cell-Secreted Protein(FDC-SP)の発現と歯周病の関連性について
  • Author(s): 高橋 伸行、福田 光男、三谷 章雄、藤村 岳樹、永原 絢子、大澤 数洋、佐藤 聡太、野口 俊英、前田 初彦、久保 勝俊、杉田 好彦
  • Organizer: 第81回愛知学院大学歯学会
  • Place of Presentation: 名古屋
• [Book] 慢性疾患としての歯周病へのアプローチ (2014)
  • Author(s): 野口俊英、林潤一郎、三谷章雄ほか
  • Total Pages: 239
  • Publisher: 医歯薬出版
• [Book] 慢性疾患としての歯周病のアプローチ 患者さんの生涯にわたるQOLに貢献するために (2014)
  • Author(s): 野口俊英、林潤一郎、三谷章雄他
  • Total Pages: 252
  • Publisher: 医歯薬出版
• [Book] 臨床歯周病学 第２版 (2013)
  • Author(s): 野口俊英、三谷章雄
  • Total Pages: 408
  • Publisher: 医歯薬出版