Elucidation of the alveolar bone absorption mechanism by the oral malodorous compounds .

• Project/Area Number: 24593171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: IMAI TOSHIO 日本歯科大学, 生命歯学部, 教授 (90120617)
• Co-Investigator(Kenkyū-buntansha): YAEGAKI Ken 日本歯科大学, 生命歯学部, 教授 (40166468) ; KAMODA Takeshi 日本歯科大学, 新潟生命歯学部, 講師 (00366767)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マウス骨髄幹細胞 / 破骨細胞 / 骨芽細胞 / 硫化水素 / 口臭 / 分化誘導 / 口臭物質 / 歯周疾患

Outline of Final Research Achievements

TThis study was aimed to elucidate the effect of hydrogen sulfide on the differentiation mechanism of bone marrow stem cells. Bone marrow stem cells were isolated from mouse femur using stem cell separation device. CD114 (+) positive cells (BMC-CD117 (+)) were isolated from the stem cells using a Magnetic separation system . NFAT1 and Aggrecan Neoepitope were present in the BMC-CD117 (+). BMC-CD117 (+) were cultured for 3 days with hydrogen sulfide. Multinucleated cells of tartrate-resistant acid phosphatase-positive that is a differentiation marker of osteoclast were significantly observed. Hydrogen sulfide was induced differentiation of osteoclasts from bone marrow stem cells, suggesting that it is a risk factor for alveolar bone resorption.

Research Products

• [Presentation] 硫化水素は骨髄幹細胞から破骨細胞を分化誘導する (2014)
  • Author(s): 今井敏夫，那須優則，八重垣 健
  • Organizer: 日本歯科基礎医学会
  • Place of Presentation: 福岡国際会議場 （ 福岡県、福岡市 ）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 揮発性硫黄化合物による骨髄間葉系幹細胞の破骨細胞への分化誘導 (2014)
  • Author(s): 今井 敏夫，伊井 久貴，岡田 実緒，那須 優則，八重垣 健
  • Organizer: 日本口腔衛生学会
  • Place of Presentation: 熊本市国際交流会館 （ 熊本県、熊本市 ）
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 乳歯歯髄幹細胞の膵臓分化における硫化水素の影響 (2014)
  • Author(s): 岡田 実緒, Nikolay Ishkitiev，八重垣 健, 今井 敏夫
  • Organizer: 日本口腔衛生学会
  • Place of Presentation: 熊本市国際交流会館 （ 熊本県、熊本市 ）
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] PKC mediate osteoclast differentiation induced by hydrogen sulfide (2014)
  • Author(s): T.Imai, H.Ii, K.Yaegaki
  • Organizer: AADR annual meeting
  • Place of Presentation: Charlotte, N.C, USA
• [Presentation] 揮発性硫黄化合物による骨髄間葉系幹細胞の破骨細胞への分化誘導 (2014)
  • Author(s): 今井敏夫、伊井久貴、岡田実緒、那須優則、八重垣健
  • Organizer: 第６３回日本口腔衛生学会
  • Place of Presentation: 熊本、熊本市国際交流会館