| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Purpose of this study is to establish biochemical assay systems for understanding of regulatory mechanisms of lysine-methylated nucleosome core particles through the histone demethylase family of LSD1/LSD2 (Lysine-specific demethylase-1/2) which is assumed in the onsets of intractable cancers and/or lifestyle-related diseases. We achieved cell-free protein production methodology to synthesize full-length histone H3 protein containing a site-specific mono-methyl-lysine, which is the demethylation substrates for LSD1/LSD2 in the context of chromatin, through the combination of site-specific incorporation of Boc-protected mono-methyl-lysine into a protein during translation and chemical removal of the Boc group.
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