2014 Fiscal Year Final Research Report
Study on nucleosomes and chromatin-demodifying enzymes
Project/Area Number |
24613007
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
epigenetics
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
UMEHARA Takashi 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (20415095)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 遺伝子発現 / エピジェネティクス / エピゲノム / クロマチン / 染色体 / ヒストン / ヌクレオソーム / 構造解析 |
Outline of Final Research Achievements |
Purpose of this study is to establish biochemical assay systems for understanding of regulatory mechanisms of lysine-methylated nucleosome core particles through the histone demethylase family of LSD1/LSD2 (Lysine-specific demethylase-1/2) which is assumed in the onsets of intractable cancers and/or lifestyle-related diseases. We achieved cell-free protein production methodology to synthesize full-length histone H3 protein containing a site-specific mono-methyl-lysine, which is the demethylation substrates for LSD1/LSD2 in the context of chromatin, through the combination of site-specific incorporation of Boc-protected mono-methyl-lysine into a protein during translation and chemical removal of the Boc group.
|
Free Research Field |
エピジェネティクス
|