Activation of dendritic cells and induction of ovalbumin-specific allergic responses by irradiation
| Project/Area Number |
24614017
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 食物アレルギー / 放射線 / 樹状細胞 / 消化管 / アレルギー / X線 / 上皮細胞 / 腸管膜リンパ球 / 共刺激分子CD40 |
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| Outline of Final Research Achievements |
We have observed that dendritic cells (DCs) of the murine intestine were resistance to X-ray irradiation and expression of costimulatory molecule, CD80 on intestinal DCs was enhanced by 10 Gy irradiation although intestinal epithelial cells were sensitive to X-ray irradiation. Then, damage-associated molecular pattern molecule (DAMP) such as HMGB1 was released into culture supernatants when mouse intestinal epithelial cells by organ culture were irradiated with 10-30 Gy. Furthermore, expression of CD80 and CD86 on intestinal DCs was enhanced after addition of HMGB1 in vitro. Thus, we have shown that DAMP such as HMGB1 is released from intestinal epithelial cells damaged by irradiation and intestinal DCs are activated by the released HMGB1. Gut DCs activated by irradiation seem to induce allergic responses and contribute to induction and progression of food allergies.
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Report
(4 results)
Research Products
(18 results)
