The efficacy of olfactory mucosa as a source of neural stem cells

• Project/Area Number: 24615008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Regenerative medicine
• Research Institution: Kawasaki Medical School
• Principal Investigator: MIYAMOTO OSAMU 川崎医科大学, 医学部, 教授 (00253287)
• Co-Investigator(Kenkyū-buntansha): HIMI Naoyuki 川崎医科大学, 医学部, 講師 (70412161) ; OKABE Naohiko 川崎医科大学, 医学部, 助教 (30614276) ; NARITA Kazuhiko 川崎医科大学, 医学部, 講師 (60104808)
• Research Collaborator: MARUYAMA Emi ; AUER Roland N
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 嗅粘膜 / 神経幹細胞 / 嗅神経鞘細胞 / 移植 / 難治性てんかん / パーキンソン病 / てんかん / キンドリングラット

Outline of Final Research Achievements

The efficacy of olfactory mucosa as a source of neural stem cells was investigated. Stem cells of olfactory epithelium were differentiated into neural cells by adding neurotrophic factors such as BDNF and GDNF to culture medium. On the other hand, olfactory ensheathing cells (OECs) were abundant in lamina propria and cultured until 70% confluent. We transplanted both neural stem cells and OECs into intractable epilepsy or Parkinson model rats. Behavioral abnormality were improved in Parkinson rats but not in epilepsy rats. The results suggest that olfactory mucosa is useful as a cellular reservoir for transplantation.

Research Products

• [Journal Article] Neuroprotection of granulocyte colony-stimulating factor during the acute phase of transient forebrain ischemia in gerbils. (2014)
  • Author(s): Lu F, Nakamura T, Toyoshima T, Liu Y, Shinomiya A, Hirooka K, Okabe N, Miyamoto O, Tamiya T, Keep RF, Itano T
  • Journal Title: Brain Res Volume: 1548 Pages: 49-55
  • DOI: 10.1016/j.brainres.2013.12.010
  • Peer Reviewed
• [Journal Article] c-Fos immunoreactivity of neural cells in intoxication due to high-dose methamphetamine (2014)
  • Author(s): Tomita M, Katsuyama H, Watanabe Y, Shibaike Y, Yoshinari H, Tee JW, Iwachidou N, Miyamoto O
  • Journal Title: J Toxicol Sci Volume: 38 Pages: 671-678
  • NAID: 10031191725
  • Peer Reviewed
• [Journal Article] Delayed administration of the nucleic acid analog 2Cl-C.OXT-A attenuates brain damage and enhances functional recovery after ischemic stroke (2013)
  • Author(s): Okabe N, Nakamura E, Himi N, Narita K, Tsukamoto I, Maruyama T, Sakakibara N, Nakamura T, Itano T, Miyamoto O
  • Journal Title: Brain Research Volume: 1506 Pages: 115-131
  • DOI: 10.1016/j.brainres.2013.02.009
  • Peer Reviewed
• [Journal Article] Calcium influx through the TRPV1 channel of endothelial cells (ECs) correlates with a stronger adhesion between monocytes and ECs. (2012)
  • Author(s): Himi N
  • Journal Title: Adv Med Sci. Volume: 57 Issue: 2 Pages: 224-229
  • DOI: 10.2478/v10039-012-0044-4
  • Peer Reviewed
• [Presentation] 脳塞栓発症後の早期運動による海馬BDNF濃度の変化と神経細胞保護に対する役割 (2015)
  • Author(s): 氷見直之, 高橋尚, 岡部直彦, 陸豊, 中村恵美, 城本高志, 成田和彦, 古我知成, 宮本修
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場（神戸）
  • Year and Date: 2015-03-25
• [Presentation] 脳梗塞後の神経新生と機能回復の関連について (2014)
  • Author(s): 城本高志、岡部直彦、陸豊、中村ー丸山恵美、氷見直之、成田和彦、木村和美、宮本修
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-09-13
• [Presentation] The exercise at early stage after stroke enhanced the recovery of memory function via BDNF elevation in the hippocampus in stroke model rats
  • Author(s): Himi N, Takahashi H, Okabe N, Koga T, Nakamura E, Feng L, Shiromoto T, Narita K and Miyamoto O
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego, USA
• [Presentation] 脳梗塞急性期における運動による認知機能回復と海馬BDNF濃度の上昇との関係
  • Author(s): 氷見直之, 高橋尚, 岡部直彦, 古我知成, 中村恵美, 成田和彦, 宮本修
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] 脳梗塞モデルラットを用いた脳梗塞急性期の運動負荷による機能回復効果の検証
  • Author(s): 氷見直之、高橋尚、中村恵美、国安勝司、岡部直彦、成田和彦、古我知成、宮本修
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
• [Presentation] 脳梗塞モデルラットにおける脳梗塞急性期の海馬BDNF濃度上昇が認知機能回復に及ぼす効果
  • Author(s): 氷見直之、高橋尚、古我知成、岡部直彦、中村恵美、成田和彦、宮本修
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] 脳虚血耐性におけるリアノジン受容体の関与
  • Author(s): 中村恵美、岡部直彦、氷見直之、成田和彦、宮本修
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀（東京都）
• [Remarks] てんかんやパーキンソン病に対する嗅粘膜細胞を使った移植研究
  • URL: http://www.kawasaki-m.ac.jp/physiology/olf.htm
• [Remarks] 川崎医科大学生理学２ 研究内容ー嗅粘膜細胞と移植
  • URL: http://www.kawasaki-m.ac.jp/physiology/res.htm
• [Remarks] 川崎医科大学生理学２
  • URL: http://www.kawasaki-m.ac.jp/physiology/index.htm