Liquid extraction surface analysis of lipids from local tissue areas by mass spectrometry for pathological analysis

• Project/Area Number: 24619007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Chubu University
• Principal Investigator: TAGUCHI Ryo 中部大学, 応用生物学部, 客員教授 (20080210)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: メタボロミクス / リピドミクス / 質量分析

Outline of Final Research Achievements

We applied a liquid extraction surface analysis (LESA) combination with NanoMate as a new approach for localization analysis of lipid molecular species in mammalian tissue slices and body fluid on segmented membrane. As results mass profiles of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in different areas of several tissue slices were effectively obtained by direct extraction. The profiles in molecular species of glycosphingolipids (gangliosides and sulfatides) and triglycerides were also effectively obtained by this method.

Research Products

• [Journal Article] Sterylglucoside catabolism in Cryptococcus neoformans with endoglycoceramidase-relataed protein2 (EGCrP2), the first steryl-b-glucosidaseidentified in fungi (2015)
  • Author(s): Watanabe T, et al.
  • Journal Title: Journal of Biological Chemistry Volume: 290 Issue: 2 Pages: 1005-1019
  • DOI: 10.1074/jbc.m114.616300
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preferential Incorporation of Shorter and Less Unsaturated Acyl Phospholipids into High Density Lipoprotein-Like Particles in the ABCA1- and ABCA7-mediated Biogenesis with apoA-I (2015)
  • Author(s): Noriko Hotta, Sumiko Abe-Dohmae, Ryo Taguchi, and Shinji Yokoyama
  • Journal Title: Chemistry and Physics of Lipids Volume: 187 Pages: 1-9
  • DOI: 10.1016/j.chemphyslip.2015.01.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Very-long-chain polyunsaturated fatty acids accumulate in phosphatidylcholine of fibroblasts from patients with Zellweger syndrome and acyl-CoA oxidase1 deficiency (2014)
  • Author(s): Yuichi Abe, Masanori Honsho, Hiroki Nakanishi, Ryo Taguchi, Yukio Fujiki
  • Journal Title: BBA - Molecular and Cell Biology of Lipids Volume: 1841 Issue: 4 Pages: 610-619
  • DOI: 10.1016/j.bbalip.2014.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of ATP citrate lyase induces triglyceride accumulation with altered fatty acid composition in cancer cells. (2014)
  • Author(s): 5.Migita T, Okabe S, Ikeda K, Igarashi S, Sugawara S, Tomida A, Soga T, Taguchi R, Seimiya H.
  • Journal Title: Int J Cancer. Volume: 135 Issue: 1 Pages: 37-47
  • DOI: 10.1002/ijc.28652
  • Peer Reviewed
• [Journal Article] The adipocyte-inducible secreted phospholipases PLA2G5 and PLA2G2E play distinct roles in obesity. (2014)
  • Author(s): Sato H, Taketomi Y, Ushida A, Isogai Y, Kojima T, Hirabayashi T, Miki Y, Yamamoto K, Nishito Y, Kobayashi T, Ikeda K, Taguchi R, Hara S, Ida S, Miyamoto Y, Watanabe M, Baba H, Miyata K, Oike Y, Gelb MH, Murakami M.
  • Journal Title: Cell Metab. Volume: 20 Issue: 1 Pages: 119-132
  • DOI: 10.1016/j.cmet.2014.05.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel Lysophospholipid Acyltransferase PLAT1 of Aurantiochytrium limacinum F26-b Responsible for Generation of Palmitate-Docosahexaenoate-Phosphatidylcholine and Phosphatidylethanolamine (2014)
  • Author(s): Eriko Abe, Kazutaka Ikeda, Eri Nutahara, Masahiro Hayashi, Atsushi Yamashita, Ryo Taguchi, Kosaku Doi, Daiske Honda, Nozomu Okino, Makoto Ito
  • Journal Title: PLoS one Volume: 9 Issue: 8 Pages: 18-81
  • DOI: 10.1371/journal.pone.0102377
  • Peer Reviewed / Open Access
• [Journal Article] 脂質メタボロミクスーリピドミクス、特集タイトル：生命を支える脂質―最新の研究と臨床 (2014)
  • Author(s): 田口 良
  • Journal Title: 医学のあゆみ Volume: Vol 248, No.13 Pages: 1205-1211
• [Journal Article] Mutation for nonsyndromic mental retardation in the trans-2-enoyl-CoA reductase TER gene involved in fatty acid elongation impairs the enzyme activity and stability, leading to change in sphingolipid profile (2013)
  • Author(s): Abe K, Ohno Y, Sassa T, Taguchi R, Çali şkan, M, Ober C, Kihara A
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 51 Pages: 36741-36749
  • DOI: 10.1074/jbc.m113.493221
  • NAID: 120005385316
  • Peer Reviewed
• [Journal Article] Inhibition of ATP citrate lyase induces an anticancer effect via reactive oxygen species: AMPK as a predictive biomarker for therapeutic impact (2013)
  • Author(s): Migita, T., Okabe, S., Ikeda, K., Igarashi, S., Sugawara, S., Tomida, A., Taguchi, R., Soga, T., Seimiya, H.
  • Journal Title: Am J Pathol. Volume: 182 Issue: 5 Pages: 1800-1810
  • DOI: 10.1016/j.ajpath.2013.01.048
  • Peer Reviewed
• [Journal Article] Mast cell maturation is driven via a novel group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis. (2013)
  • Author(s): Taketomi, Y., Morimoto, K., Murakami, M., et al., 他30名
  • Journal Title: Nature Immunol. Volume: (印刷中) Issue: 6 Pages: 554-63
  • DOI: 10.1038/ni.2586
  • Peer Reviewed
• [Journal Article] Lipidomic analysis of brain tissues and plasma in a mouse model expressing mutated human amyloid precursor protein/tau for Alzheimer's disease. (2013)
  • Author(s): Tajima Y, Ishikawa M, Maekawa K, Murayama M, Senoo Y, Nishimaki-Mogami T, Nakanishi H, Ikeda K, Arita M, Taguchi R, Okuno A, Mikawa R, Niida S, Takikawa O, Saito Y.
  • Journal Title: Lipids Health Dis Volume: 12 Issue: 1 Pages: 68-68
  • DOI: 10.1186/1476-511x-12-68
  • Peer Reviewed
• [Journal Article] Molecular Species of Phospholipids with Very Long Chain Fatty Acids in Skin Fibroblasts of Zellweger Syndrome. (2013)
  • Author(s): Hama K, Nagai T, Nishizawa C, Ikeda K, Morita M, Satoh N, Nakanishi H, Imanaka T, Shimozawa N, Taguchi R, Inoue K, Yokoyama K.
  • Journal Title: Lipids Volume: 48 Pages: 1253-1267
  • Peer Reviewed
• [Journal Article] Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators. (2013)
  • Author(s): Miki Y, Yamamoto K, Taketomi Y, Sato H, Shimo K, Kobayashi T, Ishikawa Y, Ishii T, Nakanishi H, Ikeda K, Taguchi R, Kabashima K, Arita M, Arai H, Lambeau G, Bollinger JM, Hara S, Gelb MH, Murakami M.
  • Journal Title: J Exp Med. Volume: 210 Issue: 6 Pages: 1217-1234
  • DOI: 10.1084/jem.20121887
  • Peer Reviewed
• [Journal Article] Impaired epidermal permeability barrier in mice lacking the Elovl1 gene responsible for very long-chain fatty acid production. (2013)
  • Author(s): Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A.
  • Journal Title: Mol Cell Biol Volume: 33 Issue: 14 Pages: 2787-2796
  • DOI: 10.1128/mcb.00192-13
  • Peer Reviewed
• [Journal Article] Global metabolomic analysis of heart tissue in a hamster model for dilated cardiomyopathy. (2013)
  • Author(s): Maekawa K, Hirayama A, Iwata Y, Tajima Y, Nishimaki-Mogami T, Sugawara S, Ueno N, Abe H, Ishikawa M, Murayama M, Matsuzawa Y, Nakanishi H, Ikeda K, Arita M, Taguchi R, Minamino N, Wakabayashi S, Soga T, Saito Y.
  • Journal Title: J Mol Cell Cardiol Volume: 59 Pages: 76-85
  • DOI: 10.1016/j.yjmcc.2013.02.008
  • Peer Reviewed
• [Journal Article] TLR4-MD-2 complex is negatively regulated by an endogenous ligand, globotetraosylceramide in vascular endothelial cells. (2013)
  • Author(s): Kondo, Y., Tokuda, N., Nishitani, C., Ohto, U., Akashi-TakKondo, Y., Tokuda, N., Nishitani, C., Ohto, U., Akashi-Takamura, S., Ito, Y., Uchikawa, M., Kuroki, Y., Miyake, K., Zhang, Q., Furukawa, K., Furukawa, K
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 110 Issue: 12 Pages: 4714-4719
  • DOI: 10.1073/pnas.1218508110
  • Peer Reviewed
• [Journal Article] The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza (2013)
  • Author(s): Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanebe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.
  • Journal Title: Cell Volume: 153 Issue: 1 Pages: 112-125
  • DOI: 10.1016/j.cell.2013.02.027
  • Peer Reviewed
• [Journal Article] The γ-secretase inhibitor DAPT increases the levels of gangliosides at neuritic terminals of differentiating PC12 cells. (2012)
  • Author(s): Oikawa N., Goto M., Ikeda K., Taguchi R. and Yanagisawa K.
  • Journal Title: Neurosci Lett. Volume: 525 Issue: 1 Pages: 49-53
  • DOI: 10.1016/j.neulet.2012.07.027
  • Peer Reviewed
• [Journal Article] Self-enhancement of hepatitis C virus replication by promotion of specific sphingolipid biosynthesis (2012)
  • Author(s): Hirata Y, Ikeda K, shimotohno K, et al.,
  • Journal Title: PLoS Pathog Volume: PLoS Pathog Issue: 8 Pages: e1002860-e1002860
  • DOI: 10.1371/journal.ppat.1002860
  • Peer Reviewed
• [Journal Article] LPIAT1 regulates arachidonic acid content in phosphatidylinositol and is required for cortical lamination in mice. (2012)
  • Author(s): Lee, HC et al.
  • Journal Title: Mol. Biol. Cell Volume: 23 Issue: 24 Pages: 4689-4700
  • DOI: 10.1091/mbc.e12-09-0673
  • Peer Reviewed
• [Presentation] IIF型分泌性ホスホリパーゼA2は表皮の恒常性維持および病態に寄与する (2013)
  • Author(s): 山本圭、三木寿美、佐藤真利子、武富芳隆、池田和貴、中西広樹、田口良、村上誠
  • Organizer: 第55回日本脂質生化学会
  • Place of Presentation: ホテル松島大観荘、宮城県
• [Presentation] Lipidomic analysis of brain tissues and plasma in a mouse model expressing mutated human amyloid precursor protein/tau for Alzheimer’s disease (2013)
  • Author(s): Yoko Tajima, Keiko Maekawa, Masaki Ishikawa, Mayumi Murayama, Yuya Senoo, Tomoko Nishimaki-Mogami, Hiroki Nakanishi, Kazutaka Ikeda, Makoto Arita, Ryo Taguchi, Alato Okuno, Ryuta Mikawa, Shumpei Niida, Osamu Takikawa, Yoshiro Saito
  • Organizer: The 9th Annual Conference of the Metabolomics Society
  • Place of Presentation: Glasgow Scotland
• [Presentation] 非アルコール性脂肪性肝炎モデルマウス（STAM®マウス）の肝臓における脂肪酸代謝物のメタボローム解析 (2013)
  • Author(s): 前川 京子, 上番増 喬, 田島 陽子, 石川 将己, 村山 真由子, 妹尾 勇弥, 最上（西巻）知子, 中西 広樹, 池田 和貴, 有田誠, 田口 良, 藤井 庄人, 柴崎 友一朗, 米山 博之, 南茂 隆生, 安田 和基, 斎藤 嘉朗
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] 溶媒抽出表面分析法(LESA)による臓器切片からの多価不飽和脂肪酸含有リン脂質の分析 (2013)
  • Author(s): 水野 顕智、川島祐介、小寺義男、松本博行、田口 良
  • Organizer: 第38回日本医用マススペクトル学会年会
  • Place of Presentation: 神戸市産業振興センター、兵庫県
• [Presentation] リピドミクスと質量分析 (2013)
  • Author(s): 田口 良
  • Organizer: 第55回日本脂質生化学会
  • Place of Presentation: ホテル松島大観荘、宮城県
  • Invited
• [Presentation] 非アルコール性脂肪性肝炎モデルマウス（STAMマウス）の肝臓における脂質メタボローム解析 (2013)
  • Author(s): 前川京子,上番増喬, 石川将己, 田島陽子, 妹尾勇弥, 村山真由子, 最上（西巻）知子,中西広樹,池田和貴,田口良,藤井庄人,柴崎友一朗, 米山博之,南茂隆生,安田和基, 斎藤嘉朗
  • Organizer: 日本薬学会１３３回年会
  • Place of Presentation: 横浜
• [Presentation] リピドミクスの基盤的技術と生命科学研究への適用 (2012)
  • Author(s): 田口 良
  • Organizer: 第26回日本医用マススペクトル学会東海支部講演会
  • Place of Presentation: 名古屋（名古屋大学医学部）
  • Invited
• [Presentation] 生体脂質解析における各種質量分析技術と応用例 (2012)
  • Author(s): 田口 良
  • Organizer: 第22回基礎及び最新の分析化学講習会
  • Place of Presentation: 名古屋（名古屋大学、中部大学）
  • Invited
• [Presentation] アルツハイマー病モデルマウスの脳及び血漿における脂肪酸代謝物のメタボローム解析 (2012)
  • Author(s): 前川京子, 田島陽子,石川将己, 村山真由子, 妹尾勇弥 ,最上（西巻）知子,中西 広樹,池田和貴,有田誠,田口良,キョウ建生,奥野海良人,新飯田俊平,斎藤嘉朗,滝川修
  • Organizer: 第７回メタボロームシンポジウム
  • Place of Presentation: 鶴岡
• [Presentation] 溶媒抽出方面分析法による組織切片又は薄膜からの脂質分子種の直接局在解析 (2012)
  • Author(s): 田口 良、水野顕智、水野良基、山口真奈、池田和貴
  • Organizer: 第37回日本医用マススペクトル学会
  • Place of Presentation: 名古屋
• [Presentation] 拡張型心筋症モデルハムスターの心筋における脂質代謝物のメタボローム解析 (2012)
  • Author(s): 前川京子、田島陽子、上野紀子、石川将己、村山真由子、最上知子、中西広樹、池田和貴、有田誠、田口良、岩田裕子、南野直人、若林繁夫、斎藤嘉朗
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] アルツハイマー病モデルマウスの脳組織および血漿における脂質メタボローム解析 (2012)
  • Author(s): 田島陽子,前川京子,石川将己,村山真由子,妹尾勇弥,最上（西巻）知子,中西広樹,池田和貴,有田 誠,田口良,キョウ建生,奥野海良人,新飯田俊平,滝川修,斎藤 嘉朗
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Book] Lipidomics: Technologies and Application （Ed. by Kim Ekroos） Lipidomics for elucidation of Metabolic Syndrome and related Lipid Metabolic Disorder (2012)
  • Author(s): Ryo Taguchi, Kazutaka Ikeda, Hiroki Nakanishi
  • Total Pages: 336
  • Publisher: Wiley-VCH
• [Book] 現代質量分析学（高山光男、瀧浪欣彦、早川滋雄、和田芳直 編） リピドミクス (2012)
  • Author(s): 田口 良
  • Total Pages: 514
  • Publisher: 化学同人