Liquid extraction surface analysis of lipids from local tissue areas by mass spectrometry for pathological analysis
Project/Area Number |
24619007
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
オミクス計測科学
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Research Institution | Chubu University |
Principal Investigator |
TAGUCHI Ryo 中部大学, 応用生物学部, 客員教授 (20080210)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | メタボロミクス / リピドミクス / 質量分析 |
Outline of Final Research Achievements |
We applied a liquid extraction surface analysis (LESA) combination with NanoMate as a new approach for localization analysis of lipid molecular species in mammalian tissue slices and body fluid on segmented membrane. As results mass profiles of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in different areas of several tissue slices were effectively obtained by direct extraction. The profiles in molecular species of glycosphingolipids (gangliosides and sulfatides) and triglycerides were also effectively obtained by this method.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] The adipocyte-inducible secreted phospholipases PLA2G5 and PLA2G2E play distinct roles in obesity.2014
Author(s)
3.Sato H, Taketomi Y, Ushida A, Isogai Y, Kojima T, Hirabayashi T, Miki Y, Yamamoto K, Nishito Y, Kobayashi T, Ikeda K, Taguchi R, Hara S, Ida S, Miyamoto Y, Watanabe M, Baba H, Miyata K, Oike Y, Gelb MH, Murakami M.
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Journal Title
Cell Metab.
Volume: 20
Pages: 119-132
DOI
Related Report
Peer Reviewed
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[Journal Article] Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.2013
Author(s)
Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, Tanaka S, Sakanaka M, Nakamura M, Nishito Y, Kawana M, Kambe N, Ikeda K, Taguchi R, Nakamizo S, Kabashima K, Gelb MH, Arita M, Yokomizo T, Nakamura M, 他13名
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Journal Title
Nat Immunol
Volume: 14(6)
Pages: 554-63
DOI
Related Report
Peer Reviewed
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[Journal Article] Lipidomic analysis of brain tissues and plasma in a mouse model expressing mutated human amyloid precursor protein/tau for Alzheimer's disease.2013
Author(s)
Tajima Y, Ishikawa M, Maekawa K, Murayama M, Senoo Y, Nishimaki-Mogami T, Nakanishi H, Ikeda K, Arita M, Taguchi R, Okuno A, Mikawa R, Niida S, Takikawa O, Saito Y.
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Journal Title
Lipids Health Dis
Volume: 12
Pages: 68
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular Species of Phospholipids with Very Long Chain Fatty Acids in Skin Fibroblasts of Zellweger Syndrome.2013
Author(s)
Hama K, Nagai T, Nishizawa C, Ikeda K, Morita M, Satoh N, Nakanishi H, Imanaka T, Shimozawa N, Taguchi R, Inoue K, Yokoyama K.
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Journal Title
Lipids
Volume: 48
Pages: 1253-1267
Related Report
Peer Reviewed
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[Journal Article] Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators.2013
Author(s)
Miki Y, Yamamoto K, Taketomi Y, Sato H, Shimo K, Kobayashi T, Ishikawa Y, Ishii T, Nakanishi H, Ikeda K, Taguchi R, Kabashima K, Arita M, Arai H, Lambeau G, Bollinger JM, Hara S, Gelb MH, Murakami M.
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Journal Title
J Exp Med.
Volume: 210(6)
Pages: 1217-34
DOI
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Peer Reviewed
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[Journal Article] Impaired epidermal permeability barrier in mice lacking elovl1, the gene responsible for very-long-chain fatty acid production.2013
Author(s)
Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A.
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Journal Title
Mol Cell Biol.
Volume: 33(14)
Pages: 2787-96
DOI
Related Report
Peer Reviewed
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[Journal Article] Global metabolomic analysis of heart tissue in a hamster model for dilated cardiomyopathy.2013
Author(s)
Maekawa K, Hirayama A, Iwata Y, Tajima Y, Nishimaki-Mogami T, Sugawara S, Ueno N, Abe H, Ishikawa M, Murayama M, Matsuzawa Y, Nakanishi H, Ikeda K, Arita M, Taguchi R, Minamino N, Wakabayashi S, Soga T, Saito Y.
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Journal Title
J Mol Cell Cardiol.
Volume: 59
Pages: 76-85
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Peer Reviewed
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[Journal Article] TLR4-MD-2 complex is negatively regulated by an endogenous ligand, globotetraosylceramide.2013
Author(s)
Kondo Y, Ikeda K, Tokuda N, Nishitani C, Ohto U, Akashi-Takamura S, Ito Y, Uchikawa M, Kuroki Y, Taguchi R, Miyake K, Zhang Q, Furukawa K, Furukawa K.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 110
Pages: 4714-4719
DOI
Related Report
Peer Reviewed
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[Journal Article] LPIAT1 regulates arachidonic acid content in phosphatidylinositol and is required for cortical lamination in mice2012
Author(s)
Lee HC, Inoue T, Sasaki J, Kubo T, Matsuda S, Nakasaki Y, Hattori M, Tanaka F, Udagawa O, Kono N, Itoh T, Ogiso H, Taguchi R, Arita M, Sasaki T, Arai H.
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Journal Title
Mol Biol Cell.
Volume: 23
Pages: 4689-4700
DOI
Related Report
Peer Reviewed
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[Presentation] 非アルコール性脂肪性肝炎モデルマウス(STAM®マウス)の肝臓における脂肪酸代謝物のメタボローム解析2013
Author(s)
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Organizer
第86回日本生化学会大会
Place of Presentation
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Year and Date
20130911-20130913
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[Presentation] Lipidomic analysis of brain tissues and plasma in a mouse model expressing mutated human amyloid precursor protein/tau for Alzheimer’s disease2013
Author(s)
Yoko Tajima, Keiko Maekawa, Masaki Ishikawa, Mayumi Murayama, Yuya Senoo, Tomoko Nishimaki-Mogami, Hiroki Nakanishi, Kazutaka Ikeda, Makoto Arita, Ryo Taguchi, Alato Okuno, Ryuta Mikawa, Shumpei Niida, Osamu Takikawa, Yoshiro Saito
Organizer
The 9th Annual Conference of the Metabolomics Society
Place of Presentation
Glasgow Scotland
Year and Date
20130701-20130704
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[Presentation] リピドミクスと質量分析2013
Author(s)
田口 良
Organizer
第55回日本脂質生化学会
Place of Presentation
ホテル松島大観荘、宮城県
Year and Date
20130606-20130607
Related Report
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[Presentation] 非アルコール性脂肪性肝炎モデルマウス(STAMマウス)の肝臓における脂質メタボローム解析2013
Author(s)
前川京子,上番増喬, 石川将己, 田島陽子, 妹尾勇弥, 村山真由子, 最上(西巻)知子,中西広樹,池田和貴,田口良,藤井庄人,柴崎友一朗, 米山博之,南茂隆生,安田和基, 斎藤嘉朗
Organizer
日本薬学会133回年会
Place of Presentation
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Year and Date
20130328-20130330
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[Presentation] アルツハイマー病モデルマウスの脳組織および血漿における脂質メタボローム解析2012
Author(s)
田島陽子,前川京子,石川将己,村山真由子,妹尾勇弥,最上(西巻)知子,中西広樹,池田和貴,有田 誠,田口良,キョウ建生,奥野海良人,新飯田俊平,滝川修,斎藤 嘉朗
Organizer
第85回日本生化学会大会
Place of Presentation
福岡
Year and Date
20121214-20121216
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[Presentation] アルツハイマー病モデルマウスの脳及び血漿における脂肪酸代謝物のメタボローム解析2012
Author(s)
前川京子, 田島陽子,石川将己, 村山真由子, 妹尾勇弥 ,最上(西巻)知子,中西 広樹,池田和貴,有田誠,田口良,キョウ建生,奥野海良人,新飯田俊平,斎藤嘉朗,滝川修
Organizer
第7回メタボロームシンポジウム
Place of Presentation
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Year and Date
20121010-20121012
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