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Analysis of mechanisms of resistance to cosmic radiation and genomic instability of plant

Research Project

Project/Area Number 24620001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field 宇宙生命学
Research InstitutionTohoku University

Principal Investigator

Teranishi Mika  東北大学, 生命科学研究科, 助教 (10333832)

Co-Investigator(Kenkyū-buntansha) HIDEMA Jun  東北大学, 大学院生命科学研究科, 准教授 (20250855)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords放射線 / 植物 / DNA / 損傷 / ゲノム不安定化 / 変異 / 不安定化
Outline of Final Research Achievements

In this study, we measured the number of DNA damages induced by ionizing radiation (gamma ray and x-ray) and non-ionizing radiation (UV-B radiation) in Arabidopsis seedlings. After irradiation, whole plants used for preparing an agarose plug. The agarose plugs were subjected to denature and non-denature agarose gel electrophoresis. The number of DNA double strand break (DSB), single strand break (SSB) and cyclobutane pyrimidine dimer (CPD)were calculated using the molecular length standard curve and the quantity of DNA at each migration position as shown by the quantitative image data. As a result, the number of DNA damages increased with increasing dose of radiation. DSB, SSB and CPD were induced by irradiation with gamma ray, x-ray and UV-B radiation. However, induction efficiency was different among the types of radiation. Furthermore, we constructed the transgenic Marchantia polymorpha which has marker gene as a recombination substrate for detect a homologous recombination.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2013 2012

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] UV-B-Induced CPD photolyase gene expression is regulated by UVR8-dependent and -independent pathways in Arabidopsis.2015

    • Author(s)
      Li N, Teranishi M, Yamaguchi H, Matsushita T, Watahiki MK, Tsuge T, Li SS, Hidema J
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 10 Pages: 2014-2023

    • DOI

      10.1093/pcp/pcv121

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Analysis of DNA strand breaks induced by carbon ion beams in Arabidopsis.2015

    • Author(s)
      Teranishi M, Yamaguchi H, Sakamoto AN, Hidema J
    • Journal Title

      JAEA-Review 2014

      Volume: 1 Pages: 115-115

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transport of rice cyclobutane pyrimidine dimer (CPD) photolyase into mitochondria relies on a targeting sequence located in its C-terminal internal region.2014

    • Author(s)
      Takahashi S, Teranishi M, Izumi M, Takahashi M, Takahashi F and Hidema J.
    • Journal Title

      The Plant Journal

      Volume: 79 Issue: 6 Pages: 951-963

    • DOI

      10.1111/tpj.12598

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The phosphorylation status of CPD photolyase in wild rice and other poaceae species2013

    • Author(s)
      Teranishi M, Nakamura K
    • Journal Title

      Saito Ho-on Kai Museum Research Bulletin

      Volume: 77 Pages: 1-8

    • Related Report
      2013 Research-status Report
  • [Journal Article] Over-expression of ATR causes authophagic cell death2013

    • Author(s)
      Mori C, Yamaguchi Y, Teranishi M, Takanami T, Nagase T, Kanno S, Yasui A, Higashitani A
    • Journal Title

      Genes to Cells

      Volume: 18 Issue: 4 Pages: 278-287

    • DOI

      10.1111/gtc.12034

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a phosphorylation site in cyclobutane pyrimidine dimer photolyase of rice.2013

    • Author(s)
      Teranishi M, Nakamura K, Furukawa H and Hidema J.
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 63 Pages: 24-29

    • DOI

      10.1016/j.plaphy.2012.11.003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams.2013

    • Author(s)
      Takano N, Takahashi Y, Yamamoto M, Teranishi M, Yamagushi H, Sakamoto AN, Hase Y, Fujisawa H, Wu J, Matsumoto T, Toki S, Hidema J.
    • Journal Title

      Journal of Radiation Research

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Eukaryotic Class II CPD photolyase structure reveals a basis for improved UV-tolerance in plants2012

    • Author(s)
      K.Hitomi
    • Journal Title

      J.Biol.Chem.

      Volume: 287(15) Issue: 15 Pages: 12060-9

    • DOI

      10.1074/jbc.m111.244020

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] イネにおける太陽紫外線誘発ピリミジン二量体の定量と生育に及ぼす影響2015

    • Author(s)
      寺西美佳、山口弘子、高橋祐子、日出間純
    • Organizer
      日本宇宙生物科学会第29回大会
    • Place of Presentation
      帝京大学板橋キャンパス (東京都・板橋区)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of DNA damage induced by ion beam, gamma ray and UV-B radiation in Arabidopsis2015

    • Author(s)
      Mika Teranishi, Hiroko Yamaguchi, Ayako N Sakamoto, Jun Hidema
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      国立京都国際会館 (京都府・京都市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of solar UVB radiation on rice plant and mechanisms of UVB resistance in plant2015

    • Author(s)
      Jun Hidema, Mika Teranishi, Akihisa Takahashi, Takeo Ohnishi
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      国立京都国際会館 (京都府・京都市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナにおけるイオンビーム誘発損傷の定量解析2014

    • Author(s)
      寺西美佳、山口弘子、日出間純、坂本綾子
    • Organizer
      第9回高崎量子応用研究シンポジウム
    • Place of Presentation
      群馬県高崎市高崎シティギャラリー
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 基部陸上植物ゼニゴケの太陽放射線応答に関する研究2014

    • Author(s)
      一條拓巳、寺西美佳、三岡周子、西浜竜一、河内孝之、日出間純
    • Organizer
      日本宇宙生物科学会第28回大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Year and Date
      2014-09-22 – 2014-09-23
    • Related Report
      2014 Research-status Report
  • [Presentation] イネのtriple-targeting CPD 光回復酵素のミトコンドリア移行シグナル配列に関する研究2014

    • Author(s)
      高橋さやか、寺西美佳、高橋正明、高橋文雄、日出間純
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 植物における放射線誘発DNA損傷の定量解析2013

    • Author(s)
      寺西美佳, 山口弘子, 坂本綾子、土岐精一, 日出間純
    • Organizer
      第27回日本宇宙生物科学会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Light (UV, blue, and red) regulated gene expression of CPD photolyase in Arabidopsis2013

    • Author(s)
      Nan Li, Mika Teranishi, Jun Hidema
    • Organizer
      The 6th Asia & Oceania Conference on Photobiology
    • Place of Presentation
      Australia, Sydney
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification of putative mitochondrion and nucleus targeting sequences of triple-targeted CPD photolyase protein of rice plant2013

    • Author(s)
      Sayaka Takahashi, Mika Teranishi, Masaaki Takahashi, Jun Hidema
    • Organizer
      The 6th Asia & Oceania Conference on Photobiology
    • Place of Presentation
      Australia, Sydney
    • Related Report
      2013 Research-status Report
  • [Presentation] イネの紫外線抵抗性とピリミジン二量体の生成数に関する解析2012

    • Author(s)
      寺西美佳、高橋祐子、山口弘子、日出間純
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      宮城
    • Related Report
      2012 Research-status Report
  • [Presentation] Subcellular localization of triple targeting rice CPD photolyase2012

    • Author(s)
      S Takahashi, M Teranishi, M Takahashi, J Hidema
    • Organizer
      Genetic Stability & Change: Genome Maintenance Mechanisms in Plants.
    • Place of Presentation
      フランス、ロスコフ
    • Related Report
      2012 Research-status Report
  • [Presentation] 植物を用いた太陽放射線誘発DNA損傷修復のリアルタイム観察系の検討2012

    • Author(s)
      寺西美佳、山田道子、高橋さやか、高橋文雄、日出間純
    • Organizer
      日本宇宙生物科学会
    • Place of Presentation
      徳島
    • Related Report
      2012 Research-status Report
  • [Presentation] Light (UV, blue, and red) regulated gene expression of CPD photolyase in rice and Arabidopsis.2012

    • Author(s)
      N Li, T Hirosawa, F Takahashi, M Teranishi, N Takano, J Hidema
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      韓国、済州島
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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