| Project/Area Number |
24621013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
睡眠科学
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
UCHIYAMA Nahoko 国立医薬品食品衛生研究所, 生薬部, 主任研究官 (60392297)
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| Co-Investigator(Kenkyū-buntansha) |
ARITAKE Kosuke 筑波大学, 国際統合睡眠医科学研究機構, 准教授 (70390804)
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| Co-Investigator(Renkei-kenkyūsha) |
URADE Yoshihiro 筑波大学, 国際統合睡眠医科学研究機構, 教授 (10201360)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | カンナビノイド / 睡眠 / 覚醒 / 脳波 / リポカリン型プロスタグランジンD合成酵素 / アデノシンA2A受容体 |
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| Outline of Final Research Achievements |
In this study, we focused on the effects of cannabinoids on sleep/wake regulation. In the initial study, we revealed that three synthetic cannabinoids: cannabicyclohexanol (CCH)，JWH-018 and (-)-CP-55940, dramatically decreased the locomotor activity (LOC) in wild-type (WT) mice. Subsequently, we compared the suppressive effects of the three synthetic cannabinoids on LOC in Lipocalin-type prostaglandin D synthase (L-PGDS) KO mice and adenosine A2A receptors (A2AR) KO mice, animal models of sleep/wake disorders. The suppressive effects of these cannabinoids on LOC were more potent in L-PGDS KO and/or A2AR KO mice than those of WT mice. Additionally, we tested the effects of the three cannabinoids on sleep/wake regulation evaluated by electroencephalogram (EEG) in WT and L-PGDS KO mice.These cannabinoids significantly changed the EEG power spectrum in WT as well as in L-PGDS KO mice. The power spectrum observed was different from non-rapid eye movement (non-REM) or REM sleep.
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