• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Origin and fate of earliest generated cortical neurons

Research Project

Project/Area Number 24650166
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Neuroscience in general
Research InstitutionOsaka University

Principal Investigator

MURAKAMI Fujio  大阪大学, 生命機能研究科, 教授 (20089882)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords大脳皮質 / 発生様式 / 興奮性神経細胞 / 子宮内電気穿孔 / 興奮性細胞 / ニューロン / 起源
Research Abstract

Neuroepithelial cells (NEs) in mouse cortex are thought to initially self renew and then generate basal progenitor cells (BP). Neurons are then generated from either of them by symmetric or asymmetric divisions. This idea is based on observations of mouse embryos at around E12.5 and how neurons are generated at earlier stages remains largely unknown.
Here we performed in utero electoroporation at E10.5 and observed progeny of labeled NEs in mature brain. We found that subplate cells, cortical excitatory neurons all all layers as well as astrocyes were labelled. This finding indicates that NEs generate subplate cells concomitantly with their self renewal. It also implies that these types of cells have common progenitor origins.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (30 results)

All 2013 2012 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (7 results) Remarks (4 results)

  • [Journal Article] N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration2013

    • Author(s)
      Camilla Luccardini, Laetitia Hennekinne, Lucie Viou, Mitsutoshi Yanagida, Fujio Murakami, Nicoletta Kessaris, Xufei Ma, Robert S Adelstein, René-Marc Mège, Christine Métin
    • Journal Title

      J. Neurosci

      Volume: 33 Issue: 46 Pages: 18149-60

    • DOI

      10.1523/jneurosci.0593-13.2013

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of cerebellar neurons and glias revealed by in utero electroporation : Golgi-like labeling of cerebellar neurons and glias2013

    • Author(s)
      Yoshiaki Kita, Koichi Kawakami, Yoshiko Takahashi and Fujio Murakami
    • Journal Title

      PloS one

      Volume: 8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain2013

    • Author(s)
      Masaki Shinohara, Yan Zhu, Fujio Murakami
    • Journal Title

      The Journal of comparative neurology

      Volume: 521 Issue: 14 Pages: 3340-57

    • DOI

      10.1002/cne.23353

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The control of precerebellar neuron migration by RNA-binding protein Csde1.2013

    • Author(s)
      H Kobayashi, D Kawauchi, Y Hashimoto, T Ogata, F Murakami
    • Journal Title

      Neuroscience

      Volume: 253 Pages: 292-303

    • DOI

      10.1016/j.neuroscience.2013.08.055

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The control of precerebellar neuron migration by RNA binding protein Csde12013

    • Author(s)
      Kobayashi H., Kawauchi D., Hashimoto Y., Ogata T., and Murakami F.
    • Journal Title

      Neuroscience

      Volume: 253 Pages: 292-303

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The control of precerebellar neuron migration by RNA-binding protein Csde1.2013

    • Author(s)
      H Kobayashi, D Kawauchi, Y Hashimoto, T Ogata, F Murakami.
    • Journal Title

      Neuroscience

      Volume: 253 Pages: 292-303

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration.2013

    • Author(s)
      Camilla Luccardini, Laetitia Hennekinne, Lucie Viou, Mitsutoshi Yanagida, Fujio Murakami, Nicoletta Kessaris, Xufei Ma, Robert S Adelstein
    • Journal Title

      The Journal of neuroscience : the official journal of the Society for Neuroscience

      Volume: 33 Pages: 18149-18160

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of cerebellar neurons and glias revealed by in utero electroporation: Golgi-like labeling of cerebellar neurons and glias.2013

    • Author(s)
      Yoshiaki Kita, Koichi Kawakami, Yoshiko Takahashi, Fujio Murakami.
    • Journal Title

      PloS one

      Volume: 8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain.2013

    • Author(s)
      Masaki Shinohara, Yan Zhu, Fujio Murakami.
    • Journal Title

      The Journal of comparative neurology

      Volume: 521 Pages: 3340-57

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of neuropilin-2 in the ipsilateral growth of midbrain dopaminergic axons.2013

    • Author(s)
      Makio Torigoe, Kenta Yamauchi, Atsushi Tamada, Ikuo Matsuda, Atsu Aiba, Valérie Castellani, Fujio Murakami.
    • Journal Title

      Eur J Neurosci

      Volume: 37 Pages: 1573-83

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemokine CXCL12 and its receptors in the developing central nervous system: emerging themes and future perspectives2012

    • Author(s)
      Zhu Y & Murakami F
    • Journal Title

      Developmental Neurobiology

      Volume: 72 Issue: 10 Pages: 1349-1362

    • DOI

      10.1002/dneu.22041

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation of axon-dendrite polarity in situ: Initiation of axons from polarized and non-polarized cells2012

    • Author(s)
      Yumiko Hatanaka, Kenta Yamauchi, and Fujio Murakami
    • Journal Title

      Development, Growth & Differentiation

      Volume: 54 Issue: 3 Pages: 398-407

    • DOI

      10.1111/j.1440-169x.2012.01344.x

    • NAID

      10031136912

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration2012

    • Author(s)
      Naoko Inamura, Toshiya Kimura, Satoshi Tada, Takashi Kurahashi, Mitsutoshi Yanagida, Yuchio Yanagawa, Kazuhiro Ikenaka, and Fujio Murakami
    • Journal Title

      J Neurosci

      Volume: 32 Issue: 17 Pages: 6032-6042

    • DOI

      10.1523/jneurosci.3446-11.2012

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of the leading process, nucleus and Golgi apparatus of migrating cortical interneurons in living mouse embryos2012

    • Author(s)
      Yanagida M, Miyoshi R, Toyokuni R, Zhu Y & Murakami F
    • Journal Title

      Proceedings of the National Academy of Sciences (PNAS)

      Volume: 109 Issue: 41 Pages: 16737-16742

    • DOI

      10.1073/pnas.1209166109

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain2012

    • Author(s)
      Matsui R, Tanabe Y and Watanabe D
    • Journal Title

      D, PloS one

      Volume: 7(11)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemokine CXCL12 and its receptors in the developing central nervous system: emerging themes and future perspectives.2012

    • Author(s)
      Yan Zhu
    • Journal Title

      Dev Neurobiol

      Volume: 71 Pages: 1349-62

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Formation of Axon-dendrite Polarity in situ: Initiation of Axons from Polarized and Non-polarized Cells Development2012

    • Author(s)
      Yumiko Hatanaka
    • Journal Title

      Dev Growth Differ

      Volume: 54 Pages: 398-407

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Migration of cortical interneurons in living mouse embryos: Dynamics of the leading process, nucleus and the Golgi apparatus2012

    • Author(s)
      Mitsutoshi Yanagida
    • Journal Title

      Proc Natl Acak Sci U.S.A.

      Volume: 109 Pages: 16737-42

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration.2012

    • Author(s)
      Naoko Inamura
    • Journal Title

      J Neurosci

      Volume: 32 Pages: 6032-6042

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 前脳基底部における標識細胞群から生じる皮質抑制性介在ニューロンの多様性2013

    • Author(s)
      鳥越万紀夫, 城崎航希, 村上富士夫
    • Organizer
      第36回日本神経科学大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2013-06-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 新皮質を構成するサブプレートニューロンの発生起源・分布・運命2013

    • Author(s)
      片山悠司, 萩本和也, 村上富士夫, 田辺康人
    • Organizer
      第36回日本神経科学大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2013-06-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 前脳基底部における標識細胞群から生じる皮質抑制性介在ニューロンの多様性2013

    • Author(s)
      Makio Torigoe, Koki Shirosashi, Fujio Murakami
    • Organizer
      Neuro2013
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 真皮質を構成するサブプレートニューロンの発生起源・分布・運命2013

    • Author(s)
      Yuji Katayama, Kazuya Hagimoto, Fujio Murakami, Yasuto Tanabe
    • Organizer
      Neuro2013
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 小脳脳室帯・菱脳唇由来細胞の分布パターン2012

    • Author(s)
      喜多善亮, 村上富士夫
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] ネトリン1欠損マウスにおける後脳交連軸索の末梢への伸長2012

    • Author(s)
      山内健太, 山崎真弥, 崎村建司, 梅林真由, 村上富士夫
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 中脳ドーパミン作動性ニューロン軸索の非交差性投射におけるニューロピリン2の役割2012

    • Author(s)
      鳥越万紀夫, 山内健太, 玉田篤史, 松田育雄, 餐場篤 村上富士夫
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-18
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/murakami-lab/

    • Related Report
      2013 Final Research Report
  • [Remarks] 脳ができあがるまで(基本編)

    • URL

      http://square.umin.ac.jp/murakami-lab/ippan/website%20files/home_wbs.html

    • Related Report
      2012 Research-status Report
  • [Remarks] 神経細胞が軸索を伸ばすとき、過渡的にウニのような形態を示す

    • URL

      http://www.fbs.osaka-u.ac.jp/gcoe/jp/events/achievement/cortical/

    • Related Report
      2012 Research-status Report
  • [Remarks] 神経細胞の移動する様子を生きたマウス胎仔で観察することに成功ー胎生期における脳形成の理解に貢献ー

    • URL

      http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/09/20120925_1

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi