Origin and fate of earliest generated cortical neurons

• Project/Area Number: 24650166
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Osaka University
• Principal Investigator: MURAKAMI Fujio 大阪大学, 生命機能研究科, 教授 (20089882)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 大脳皮質 / 発生様式 / 興奮性神経細胞 / 子宮内電気穿孔 / 興奮性細胞 / ニューロン / 起源

Research Abstract

Neuroepithelial cells (NEs) in mouse cortex are thought to initially self renew and then generate basal progenitor cells (BP). Neurons are then generated from either of them by symmetric or asymmetric divisions. This idea is based on observations of mouse embryos at around E12.5 and how neurons are generated at earlier stages remains largely unknown.
Here we performed in utero electoroporation at E10.5 and observed progeny of labeled NEs in mature brain. We found that subplate cells, cortical excitatory neurons all all layers as well as astrocyes were labelled. This finding indicates that NEs generate subplate cells concomitantly with their self renewal. It also implies that these types of cells have common progenitor origins.

Research Products

• [Journal Article] N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration (2013)
  • Author(s): Camilla Luccardini, Laetitia Hennekinne, Lucie Viou, Mitsutoshi Yanagida, Fujio Murakami, Nicoletta Kessaris, Xufei Ma, Robert S Adelstein, René-Marc Mège, Christine Métin
  • Journal Title: J. Neurosci Volume: 33 Issue: 46 Pages: 18149-60
  • DOI: 10.1523/jneurosci.0593-13.2013
  • Peer Reviewed
• [Journal Article] Development of cerebellar neurons and glias revealed by in utero electroporation : Golgi-like labeling of cerebellar neurons and glias (2013)
  • Author(s): Yoshiaki Kita, Koichi Kawakami, Yoshiko Takahashi and Fujio Murakami
  • Journal Title: PloS one Volume: 8
  • Peer Reviewed
• [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain (2013)
  • Author(s): Masaki Shinohara, Yan Zhu, Fujio Murakami
  • Journal Title: The Journal of comparative neurology Volume: 521 Issue: 14 Pages: 3340-57
  • DOI: 10.1002/cne.23353
  • Peer Reviewed
• [Journal Article] The control of precerebellar neuron migration by RNA-binding protein Csde1. (2013)
  • Author(s): H Kobayashi, D Kawauchi, Y Hashimoto, T Ogata, F Murakami
  • Journal Title: Neuroscience Volume: 253 Pages: 292-303
  • DOI: 10.1016/j.neuroscience.2013.08.055
  • Peer Reviewed
• [Journal Article] The control of precerebellar neuron migration by RNA binding protein Csde1 (2013)
  • Author(s): Kobayashi H., Kawauchi D., Hashimoto Y., Ogata T., and Murakami F.
  • Journal Title: Neuroscience Volume: 253 Pages: 292-303
  • Peer Reviewed
• [Journal Article] The control of precerebellar neuron migration by RNA-binding protein Csde1. (2013)
  • Author(s): H Kobayashi, D Kawauchi, Y Hashimoto, T Ogata, F Murakami.
  • Journal Title: Neuroscience Volume: 253 Pages: 292-303
  • Peer Reviewed
• [Journal Article] N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration. (2013)
  • Author(s): Camilla Luccardini, Laetitia Hennekinne, Lucie Viou, Mitsutoshi Yanagida, Fujio Murakami, Nicoletta Kessaris, Xufei Ma, Robert S Adelstein
  • Journal Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 33 Pages: 18149-18160
  • Peer Reviewed
• [Journal Article] Development of cerebellar neurons and glias revealed by in utero electroporation: Golgi-like labeling of cerebellar neurons and glias. (2013)
  • Author(s): Yoshiaki Kita, Koichi Kawakami, Yoshiko Takahashi, Fujio Murakami.
  • Journal Title: PloS one Volume: 8
  • Peer Reviewed
• [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain. (2013)
  • Author(s): Masaki Shinohara, Yan Zhu, Fujio Murakami.
  • Journal Title: The Journal of comparative neurology Volume: 521 Pages: 3340-57
  • Peer Reviewed
• [Journal Article] Role of neuropilin-2 in the ipsilateral growth of midbrain dopaminergic axons. (2013)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Atsushi Tamada, Ikuo Matsuda, Atsu Aiba, Valérie Castellani, Fujio Murakami.
  • Journal Title: Eur J Neurosci Volume: 37 Pages: 1573-83
  • Peer Reviewed
• [Journal Article] Chemokine CXCL12 and its receptors in the developing central nervous system: emerging themes and future perspectives (2012)
  • Author(s): Zhu Y & Murakami F
  • Journal Title: Developmental Neurobiology Volume: 72 Issue: 10 Pages: 1349-1362
  • DOI: 10.1002/dneu.22041
  • Peer Reviewed
• [Journal Article] Formation of axon-dendrite polarity in situ: Initiation of axons from polarized and non-polarized cells (2012)
  • Author(s): Yumiko Hatanaka, Kenta Yamauchi, and Fujio Murakami
  • Journal Title: Development, Growth & Differentiation Volume: 54 Issue: 3 Pages: 398-407
  • DOI: 10.1111/j.1440-169x.2012.01344.x
  • NAID: 10031136912
  • Peer Reviewed
• [Journal Article] Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration (2012)
  • Author(s): Naoko Inamura, Toshiya Kimura, Satoshi Tada, Takashi Kurahashi, Mitsutoshi Yanagida, Yuchio Yanagawa, Kazuhiro Ikenaka, and Fujio Murakami
  • Journal Title: J Neurosci Volume: 32 Issue: 17 Pages: 6032-6042
  • DOI: 10.1523/jneurosci.3446-11.2012
  • Peer Reviewed
• [Journal Article] Dynamics of the leading process, nucleus and Golgi apparatus of migrating cortical interneurons in living mouse embryos (2012)
  • Author(s): Yanagida M, Miyoshi R, Toyokuni R, Zhu Y & Murakami F
  • Journal Title: Proceedings of the National Academy of Sciences (PNAS) Volume: 109 Issue: 41 Pages: 16737-16742
  • DOI: 10.1073/pnas.1209166109
  • Peer Reviewed
• [Journal Article] Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain (2012)
  • Author(s): Matsui R, Tanabe Y and Watanabe D
  • Journal Title: D, PloS one Volume: 7(11)
  • Peer Reviewed
• [Journal Article] Chemokine CXCL12 and its receptors in the developing central nervous system: emerging themes and future perspectives. (2012)
  • Author(s): Yan Zhu
  • Journal Title: Dev Neurobiol Volume: 71 Pages: 1349-62
  • Peer Reviewed
• [Journal Article] Formation of Axon-dendrite Polarity in situ: Initiation of Axons from Polarized and Non-polarized Cells Development (2012)
  • Author(s): Yumiko Hatanaka
  • Journal Title: Dev Growth Differ Volume: 54 Pages: 398-407
  • Peer Reviewed
• [Journal Article] Migration of cortical interneurons in living mouse embryos: Dynamics of the leading process, nucleus and the Golgi apparatus (2012)
  • Author(s): Mitsutoshi Yanagida
  • Journal Title: Proc Natl Acak Sci U.S.A. Volume: 109 Pages: 16737-42
  • Peer Reviewed
• [Journal Article] Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration. (2012)
  • Author(s): Naoko Inamura
  • Journal Title: J Neurosci Volume: 32 Pages: 6032-6042
  • Peer Reviewed
• [Presentation] 前脳基底部における標識細胞群から生じる皮質抑制性介在ニューロンの多様性 (2013)
  • Author(s): 鳥越万紀夫, 城崎航希, 村上富士夫
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2013-06-21
• [Presentation] 新皮質を構成するサブプレートニューロンの発生起源・分布・運命 (2013)
  • Author(s): 片山悠司, 萩本和也, 村上富士夫, 田辺康人
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2013-06-21
• [Presentation] 前脳基底部における標識細胞群から生じる皮質抑制性介在ニューロンの多様性 (2013)
  • Author(s): Makio Torigoe, Koki Shirosashi, Fujio Murakami
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館
• [Presentation] 真皮質を構成するサブプレートニューロンの発生起源・分布・運命 (2013)
  • Author(s): Yuji Katayama, Kazuya Hagimoto, Fujio Murakami, Yasuto Tanabe
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館
• [Presentation] 小脳脳室帯・菱脳唇由来細胞の分布パターン (2012)
  • Author(s): 喜多善亮, 村上富士夫
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-20
• [Presentation] ネトリン1欠損マウスにおける後脳交連軸索の末梢への伸長 (2012)
  • Author(s): 山内健太, 山崎真弥, 崎村建司, 梅林真由, 村上富士夫
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-18
• [Presentation] 中脳ドーパミン作動性ニューロン軸索の非交差性投射におけるニューロピリン2の役割 (2012)
  • Author(s): 鳥越万紀夫, 山内健太, 玉田篤史, 松田育雄, 餐場篤 村上富士夫
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-18
• [Remarks]
  • URL: http://square.umin.ac.jp/murakami-lab/
• [Remarks] 脳ができあがるまで（基本編）
  • URL: http://square.umin.ac.jp/murakami-lab/ippan/website%20files/home_wbs.html
• [Remarks] 神経細胞が軸索を伸ばすとき、過渡的にウニのような形態を示す
  • URL: http://www.fbs.osaka-u.ac.jp/gcoe/jp/events/achievement/cortical/
• [Remarks] 神経細胞の移動する様子を生きたﾏｳｽ胎仔で観察することに成功ー胎生期における脳形成の理解に貢献ー
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/09/20120925_1