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A novel molecular mechanism underlying the sense of oily odorants by olfaction

Research Project

Project/Area Number 24650170
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Neuroscience in general
Research InstitutionNara Medical University

Principal Investigator

TSUBOI Akio  奈良県立医科大学, 医学部, 教授 (20163868)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiroo  奈良県立医科大学, 医学部, 助教 (20390685)
YOSHIHARA Sei-ichi  奈良県立医科大学, 医学部, 助教 (90360669)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords嗅覚系 / 油センシング / 匂いセンサー / カルシウムイメージング / 不飽和長鎖脂肪酸 / 必須脂肪酸
Research Abstract

About mechanisms that animals feel oil, there are some reports for oil sensors in gustation; however, the perception of oil by olfaction remains to be uncovered. It is possible that the perception of the oily smell by olfaction is necessary so that animals consume oil effectively in the natural world.
In this study, we focused on olfactory sensory neurons (OSNs) and sensor molecules that respond to unsaturated long-chain fatty acids to elucidate a novel molecular mechanism of oil sensing by olfaction. Notably, we not only analyzed mice by behavior experiments, but also examined responses to oily odorants in OSNs by the calcium imaging to clarify the difference in responsiveness to odorants among several kinds of oils and unsaturated long-chain fatty acids. In addition, we searched for the genes expressing specifically in the oil sensor OSNs, which we have recently found, to clarify the function of oil sensors by ectopic expression of candidate molecules in OSNs with the lentivirus.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (36 results)

All 2014 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (21 results) (of which Invited: 5 results) Book (4 results) Remarks (3 results)

  • [Journal Article] 嗅覚系におけるCO2センシングの分子機構2013

    • Author(s)
      高橋弘雄, 坪井昭夫
    • Journal Title

      化学と生物

      Volume: 第51巻 Pages: 437-439

    • NAID

      130007602381

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 嗅覚系におけるCO2センシングの分子機構2013

    • Author(s)
      高橋弘雄、坪井昭夫
    • Journal Title

      化学と生物

      Volume: 51 Pages: 437-439

    • NAID

      130007602381

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 5T4 glycoprotein regulates the sensory input- dependent development of a specific subtype of newborn interneuron in the olfactory bulb2012

    • Author(s)
      Yoshihara S, Takahashi H, Naritsuka H, Nishimura N, Shirao T, Torashima T, Hirai H, Yoshihara Y, Mori K, Stern P.L. and Tsuboi A
    • Journal Title

      J . Neurosci.

      Volume: 32 Pages: 2217-2226

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 嗅球における感覚入力依存的な神経回路再編の分子機構2012

    • Author(s)
      吉原誠一, 坪井昭夫
    • Journal Title

      AROMA RESEARCH

      Volume: 第13巻, 第3号 Pages: 235-239

    • NAID

      40019412436

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 嗅覚系における神経回路形成とCO_2センシングの分子機構2012

    • Author(s)
      高橋弘雄, 坪井昭夫
    • Journal Title

      日本応用酵素協会誌

      Volume: 第46巻 Pages: 23-30

    • Related Report
      2013 Final Research Report
  • [Journal Article] 5T4 Glycoprotein regulates the sensory input-dependent development of a specific subtype of newborn interneurons in the mouse olfactory bulb2012

    • Author(s)
      Yoshihara S, Takahashi H, Nishimura N, Naritsuka H, Shirao T, Hirai H, Yoshihara Y, Mori K, Stern P. L. and Tsuboi A
    • Journal Title

      The Journal of Neuroscience

      Volume: Vol.32 Issue: 6 Pages: 2217-2226

    • DOI

      10.1523/jneurosci.5907-11.2012

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 嗅覚系における神経回路形成とCO2センシングの分子機構2012

    • Author(s)
      高橋弘雄、坪井昭夫
    • Journal Title

      日本応用酵素協会誌

      Volume: 46 Pages: 23-30

    • Related Report
      2012 Research-status Report
  • [Journal Article] 嗅球における感覚入力依存的な神経回路再編の分子機構2012

    • Author(s)
      吉原誠一、坪井昭夫
    • Journal Title

      AROMA RESEARCH

      Volume: 13 Pages: 235-239

    • NAID

      40019412436

    • Related Report
      2012 Research-status Report
  • [Presentation] 5T4 and Npas4 regulate the sensory experience-dependent development of dendrites in newborn olfactory bulb interneurons2014

    • Author(s)
      Tsuboi A, Takahashi H, Asahina R, Kinoshita M, Nishimura N and Yoshihara S
    • Organizer
      Keystone Symposia : Adult Neurogenesis
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2013 Final Research Report
  • [Presentation] Npas4 regulates the sensory experience-dependent development of dendritic spines in newborn olfactory bulb interneuron2014

    • Author(s)
      Yoshihara S, Takahashi H, Nishimura N, Kinoshita M, Asahina R, Furukawa-Hibi Y, Nagai T, Yamada K and Tsuboi A
    • Organizer
      Cold Spring Harbor Meeting : Neuronal Cir cuits
    • Place of Presentation
      Cold Spring Harbor, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Npas4 regulates the sensory experience-dependent development of dendritic spines in newborn olfactory bulb interneuron.2014

    • Author(s)
      Yoshihara S, Takahashi H, Nishimura N, Kinoshita M, Asahina R, Furukawa-Hibi Y, Nagai T, Yamada K and Tsuboi A
    • Organizer
      Cold Spring Harbor Meeting: Neuronal Circuits
    • Place of Presentation
      Cold Spring Harbor, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 5T4 and Npas4 regulate the sensory experience-dependent development of dendrites in newborn olfactory bulb interneurons.2014

    • Author(s)
      Tsuboi A, Takahashi H, Asahina R, Kinoshita M, Nishimura N and Yoshihara S
    • Organizer
      Keystone Symposia: Adult Neurogenesis
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2013 Annual Research Report
  • [Presentation] Time-lapse imaging of neuronal migration in the mouse olfactory bulb2013

    • Author(s)
      Tsuboi A, Yoshihara S, Tamada Y and Takahashi H
    • Organizer
      In : iCheMS Symposia : The 14^<th> Inter national Membrane Resear ch Forum
    • Place of Presentation
      Kyoto Univ., Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Npas4 regulates sensory experience- dependent development of dendritic spines in olfactory bulb granule cells2013

    • Author(s)
      Yoshihara S, Takahashi H, Kinoshita M, Nishimura N Nagai T, Yamada K and Tsuboi A
    • Organizer
      Neur ogenesis 2013 in Matsushima
    • Place of Presentation
      Miyagi, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Npas4 transcription factor regulates the sensory experience-dependent dendritic spine development of newborn interneurons in the mouse olfactory bulb2013

    • Author(s)
      Tsuboi A, Takahashi H, Kinoshita M, Nishimura N and Yoshihara S
    • Organizer
      Cell Symposia : Genes, Cir cuits & Behaviour
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] Sensory experience regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb2013

    • Author(s)
      Tsuboi A, Takahashi H, Kinoshita M, Nishimura N and Yoshihara S
    • Organizer
      Keystone Symposia : Neur ogenesis
    • Place of Presentation
      Santa Fe, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Npas4 transcription factor regulates the sensory experience-dependent dendritic spine development of newborn interneurons in the mouse olfactory bulb.2013

    • Author(s)
      Tsuboi A, Takahashi H, Kinoshita M, Nishimura N and Yoshihara S
    • Organizer
      Cell Symposia: Genes, Circuits & Behaviour
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Npas4 regulates sensory experience-dependent development of dendritic spines in olfactory bulb granule cells.2013

    • Author(s)
      Yoshihara S, Takahashi H, Kinoshita M, Nishimura N Nagai T, Yamada K and Tsuboi A
    • Organizer
      Neurogenesis 2013 in Matsushima
    • Place of Presentation
      Miyagi, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sensory experience regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb.2013

    • Author(s)
      Tsuboi A, Takahashi H, Kinoshita M, Nishimura N, Yoshihara S
    • Organizer
      Keystone Symposia: Neurogenesis
    • Place of Presentation
      Santa Fe, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Time-lapse imaging of neuronal migration in the mouse olfactory bulb.2013

    • Author(s)
      Tsuboi A
    • Organizer
      アイセムス(iCeMS)シンポジウム: 第14回国際細胞膜研究フォーラム
    • Place of Presentation
      京都大学、京都
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Molecular Basis of CO2 Sensing in the Mouse Olfactory System.2013

    • Author(s)
      Tsuboi A, Yoshihara S, Tamada Y, Hirota J, Sato T, Takahashi H
    • Organizer
      第90回日本生理学会大会 公募シンポジウム「気体分子のセンシングと生理機能」
    • Place of Presentation
      タワーホール船堀、東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Sensory input regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb2012

    • Author(s)
      Tsuboi A, Takahashi H, Nishimura N, Kinoshita M, Mori K, Stern PL and Yoshihara S
    • Organizer
      In : The 16th Inter national Symposium on Olfaction and Taste (ISOT)
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2013 Final Research Report
  • [Presentation] Sensory input regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb2012

    • Author(s)
      Yoshihara S, Takahashi H, Kinoshita M, Nishimura N and Tsuboi A
    • Organizer
      Cold Spring Harbor Meeting : Axon Guidance, Synapse For mation & Regener ation
    • Place of Presentation
      Cold Spring Harbor, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular basis of CO2 sensing in the mouse olfactory system2012

    • Author(s)
      Takahashi H, Yoshihara S, Miyazaki N, Nanaura H, Hirono J, Sato T and Tsuboi A
    • Organizer
      The 16th Inter national Symposium on Olfaction and Taste (ISOT)
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2013 Final Research Report
  • [Presentation] Sensory input regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb2012

    • Author(s)
      Tsuboi A, Takahashi H, Yamada K, Mori K, Stern PL and Yoshihara S
    • Organizer
      In : Symposium"Molecular basis of odor processing in the brain", Neuroscience 2012
    • Place of Presentation
      Nagoya Congress Center
    • Related Report
      2013 Final Research Report
  • [Presentation] Sensory input regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb.2012

    • Author(s)
      Tsuboi A, Takahashi H, Nishimura N, Kinoshita M, Mori K, Stern PL, Yoshihara S
    • Organizer
      The 16th International Symposium on Olfaction and Taste (ISOT)
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 嗅球顆粒細胞における樹状突起の感覚入力依存的な発達機構2012

    • Author(s)
      坪井昭夫
    • Organizer
      平成24年度 文科省包括型脳科学研究推進支援ネットワーク  支援ワークショップ 「嗅覚情報処理の神経基盤 - 匂い分子から嗅覚神経回路、行動・情動まで -」
    • Place of Presentation
      東京大学、東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Sensory input regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb.2012

    • Author(s)
      Yoshihara S, Takahashi H, Kinoshita M, Nishimura N, Tsuboi A
    • Organizer
      Cold Spring Harbor Meeting: Axon Guidance, Synapse Formation & Regeneration
    • Place of Presentation
      Cold Spring Harbor, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Sensory input regulates the dendritic development of specific neuronal subtypes in the mouse olfactory bulb.2012

    • Author(s)
      Tsuboi A, Takahashi H, Yamada K, Mori K, Stern PL, Yoshihara S
    • Organizer
      第35回日本神経科学大会 公募シンポジウム「高次嗅覚情報処理の分子基盤」
    • Place of Presentation
      名古屋国際会議場、名古屋
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] Odorant receptor gene regulation. Handbook of Olfaction & Gustation (3^r d edition): Moder n Per spectives (editor, Doty, R.L.)2014

    • Author(s)
      Tsuboi A and Sakano H
    • Publisher
      Wiley-Blackwell Publishing(in press)
    • Related Report
      2013 Final Research Report
  • [Book] Handbook of Olfaction & Gustation (3rd edition): Modern Perspectives (editor, Doty, RL)2014

    • Author(s)
      Tsuboi A and Sakano H
    • Total Pages
      1500
    • Publisher
      Wiley-Blackwell Publishing
    • Related Report
      2013 Annual Research Report
  • [Book] 嗅覚系における匂い地図の形成機構.嗅覚と匂い・香りの産業利用最前線2013

    • Author(s)
      坪井昭夫
    • Publisher
      NTS出版
    • Related Report
      2013 Final Research Report
  • [Book] 嗅覚と匂い・香りの産業利用最前線 第1章 第6節 嗅覚系における「匂い地図」の形成機構2013

    • Author(s)
      坪井昭夫
    • Publisher
      NTS出版
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.naramed-u.ac.jp/~amrc-lab1/

    • Related Report
      2013 Final Research Report
  • [Remarks] 奈良県立医科大学 先端医学研究機構 脳神経システム医科学

    • URL

      http://www.naramed-u.ac.jp/~amrc-lab1/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 奈良県立医科大学 先端医学研究機構 脳神経システム医科学

    • URL

      http://www.naramed-u.ac.jp/~amrc-lab1/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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