Approaching the mechanisms of dendritic formation using in vivo two-photon imaging
| Project/Area Number |
24650171
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Keio University |
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Principal Investigator |
TAKEO Yukari 慶應義塾大学, 医学部, 特任助教 (90624320)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 樹状突起 / in vivoイメージング / プルキンエ細胞 / 神経科学 / 小脳 / 形態形成 / 神経活動 |
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| Research Abstract |
This study explored mechanisms which underlie how mammalian dendrites grow in vivo during neural development. Using cerebellar Purkinje cells which develop their dendrites postnatally, I investigated molecular mechanisms of dendritogenesis during 2nd postnatal week.I found that RORalpha is crucial for formation of mature dendritic branch and spines, while neuronal activities are required for stem dendrite pruning and formation of monoplanar architecture of dendritic tree.Furthermore, live imaging using two-photon microscopy revealed dynamic remodeling of developing dendrite in vivo.
These results provide insights into in vivo mechanisms of dendrite formation.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Activity-dependent proteolytic cleavage of neuroligin-12012
Author(s)
Suzuki K, Hayashi Y, Nakahara S, Kumazaki H, Prox J, Horiuchi K, Zeng M, Tanimura S, Nishiyama Y, Osawa S, Sehara-Fujisawa A, Saftig P, Yokoshima S, Fukuyama T, Matsuki N, Koyama R, Tomita T, Iwatsubo T.
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Journal Title
Neuron
Volume: 76(2)
Issue: 2
Pages: 410-422
DOI
Related Report
Peer Reviewed
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