Approaching the mechanisms of dendritic formation using in vivo two-photon imaging

• Project/Area Number: 24650171
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: TAKEO Yukari 慶應義塾大学, 医学部, 特任助教 (90624320)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 樹状突起 / in vivoイメージング / プルキンエ細胞 / 神経科学 / 小脳 / 形態形成 / 神経活動

Research Abstract

This study explored mechanisms which underlie how mammalian dendrites grow in vivo during neural development. Using cerebellar Purkinje cells which develop their dendrites postnatally, I investigated molecular mechanisms of dendritogenesis during 2nd postnatal week.I found that RORalpha is crucial for formation of mature dendritic branch and spines, while neuronal activities are required for stem dendrite pruning and formation of monoplanar architecture of dendritic tree.Furthermore, live imaging using two-photon microscopy revealed dynamic remodeling of developing dendrite in vivo.
These results provide insights into in vivo mechanisms of dendrite formation.

Research Products

• [Journal Article] Selective and regulated gene expression in murine Purkinje cells by in utero electroporation (2012)
  • Author(s): Nishiyama J, Hayashi Y, Nomura T, Miura E, Kakegawa W, Yuzaki M
  • Journal Title: European Journal of Neuroscience Volume: 36 Pages: 2867-2876
  • Peer Reviewed
• [Journal Article] Activity-dependent proteolytic cleavage of neuroligin-1 (2012)
  • Author(s): Suzuki K, Hayashi Y, Nakahara S, Kumazaki H, Prox J, Horiuchi K, Zeng M, Tanimura S, Nishiyama Y, Osawa S, Sehara-Fujisawa A, Saftig P, Yokoshima S, Fukuyama T, Matsuki N, Koyama R, Tomita T, Iwatsubo T.
  • Journal Title: Neuron Volume: 76(2) Issue: 2 Pages: 410-422
  • DOI: 10.1016/j.neuron.2012.10.003
  • Peer Reviewed
• [Presentation] Stage-specific roles of ROR in morphogenesis and maintenance of dendrites in the developing and mature Purkinje cell (2013)
  • Author(s): Yukari H Takeo, Eriko Miura, Wataru Kakegawa and Michisuke Yuzaki
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2013-06-21
• [Presentation] マウスプルキンエ細胞樹状突起形態形成および維持におけるRORalphaの時期特異的役割 (2013)
  • Author(s): 竹尾 ゆかり
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館
• [Presentation] マウスプルキンエ細胞樹状突起形態形成および維持におけるRORαの時期特異的役割
  • Author(s): 竹尾 ゆかり
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館