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Analysis of immunotransporter function of the hydrophobized polysaccharide-based nanogel and its application to cancer therapy

Research Project

Project/Area Number 24650259
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionMie University

Principal Investigator

HARADA Naozumi  三重大学, 医学(系)研究科(研究院), リサーチアソシエイト (40520961)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsナノゲル / デリバリーシステム / リンパ節 / がんワクチン / マクロファージ
Research Abstract

Hydrophobic polysaccharide was synthesized by partially modifying hydrophilic polysaccharide with cholesterol groups. In aqueous solution, it forms a nanogel with a diameter of ~50 nm, in which proteins or peptides can be embedded. In this study, we created a cancer vaccine consisting of the nanogel and a tumor antigen peptide, and the mechanism of antigen delivery function of the nanogel was investigated using mice. The nanogel efficiently transported antigen to unique subsets of lymph node macrophages with high antigen-presenting activity, at least partly in a cell-independent. This macrophage-oriented vaccine system showed an enhanced anti-tumor effect and immunogenicity, as compared to a conventional one.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (17 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients2013

    • Author(s)
      Kageyama S, Wada H, Muro K, Niwa Y, Ueda S, Miyata H, Takiguchi S, Sugino SH, Miyahara Y, Ikeda H, Imai N, Sato E, Yamada T, Osako M, Ohnishi M, Harada N, Hishida T, Doki Y, Shiku H
    • Journal Title

      Journal of translational medicine

      Volume: 5;11 Issue: 1 Pages: 246-246

    • DOI

      10.1186/1479-5876-11-246

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of animal models to analyze the kinetics and distribution of human tumor antigen-specific CD8+ T cells2013

    • Author(s)
      Muraoka D, Nishikawa H, Noguchi T, Wang L, Harada N, Sato E, Luescher I, Nakayama E, Kato T, Shiku H
    • Journal Title

      Vaccine

      Volume: 31(17) Issue: 17 Pages: 2110-2118

    • DOI

      10.1016/j.vaccine.2013.02.056

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] A Novel Cancer Vaccine with Nanogel-based Antigen Transporter and Sequence-optimized Long Peptide Antigen2014

    • Author(s)
      Harada N, Muraoka D, Hayashi T, Momose F, Tahara Y, Sawada S, Akiyoshi K, Shiku H.
    • Organizer
      ITOC-1
    • Place of Presentation
      ミュンヘン,ドイツ
    • Related Report
      2013 Final Research Report
  • [Presentation] A Novel Cancer Vaccine with Nanogel-based Antigen Transporter and Sequence-optimized Long Peptide Antigen2014

    • Author(s)
      Naozumi Harada1,2, Daisuke Muraoka1,2, Tae Hayashi1,2, Fumiyasu Momose1, Yoshiro Tahara3,4, Shin-ichi Sawada3,4, Kazunari Akiyoshi3,4, and Hiroshi Shiku1
    • Organizer
      ITOC-1
    • Place of Presentation
      ドイツ ミュンヘン
    • Related Report
      2013 Annual Research Report
  • [Presentation] Macrophage is a good target as antigen presenting cell to induce strong immune responses elicited with administration of vaccination antigen and immunological adjuvant2013

    • Author(s)
      Muraoka D, Harada N, Hayashi T, Momose F, Sawada S, Akiyoshi K, Shiku H
    • Organizer
      日本免疫学会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] Peptide Sequence Design and Delivery System Are Critical for the Immunogenicity of Long Peptide-Based Cancer Vaccines2013

    • Author(s)
      Muraoka D, Harada N, Hayashi T, Akiyoshi K, Shiku, H.
    • Organizer
      日本癌学会
    • Place of Presentation
      パシフィコ横浜,横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗原デリバリーシステム・CHPナノゲルはワクチン抗原をリンパ節内の抗原提示細胞に高効率輸送しワクチン性能を向上させる2013

    • Author(s)
      原田直純,村岡大輔,林妙,澤田晋一,秋吉一成,珠玖洋
    • Organizer
      日本DDS学会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] CHPナノゲルはロングペプチド抗原をリンパ節内のマクロファージに抗原提示させる2013

    • Author(s)
      村岡大輔,原田直純,林妙,百瀬文康,澤田晋一,秋吉一成,珠玖洋
    • Organizer
      日本がん免疫学会
    • Place of Presentation
      宇部
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] マクロファージ指向型がんワクチンの有用性検討2013

    • Author(s)
      村岡 大輔1,3、原田 直純1,3、林 妙1,3、百瀬 文康1、澤田 晋一 4、秋吉 一成4、珠玖 洋1,2
    • Organizer
      日本がん免疫学会
    • Place of Presentation
      宇部市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Peptide Sequence Design and Delivery System Are Critical for the Immunogenicity of Long Peptide-Based Cancer Vaccines2013

    • Author(s)
      Daisuke Muraoka1,3, Naozumi Harada1,3, Tae Hayashi1,3, Kazunari Akiyoshi4, and Hiroshi Shiku1,2
    • Organizer
      日本癌学会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Macrophage is a good target as antigen presenting cell to induce strong immune responses elicted with administration of vaccination antigen and immunological adjuvant2013

    • Author(s)
      Daisuke Muraoka1,3, Naozumi Harada1,3, Tae Hayashi1,3, Momose Fumiyasu2, Shin-ichi Sawada4, Kazunari Akiyoshi4, and Hiroshi Shiku1,2
    • Organizer
      日本免疫学会
    • Place of Presentation
      千葉市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 抗原デリバリーシステム・CHPナノゲルはワクチン抗原をリンパ節内の抗原提示細胞2013

    • Author(s)
      原田 直純、村岡 大輔、林 妙、澤田 晋一 、秋吉 一成、珠玖 洋
    • Organizer
      日本DDS学会
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Control of in vivo spatiotemporal dynamics of antigen and adjuvant by a delivery system CHP nanogel markedly improves the immunogenicity and antitumor efficacy of long peptide cancer vaccine2012

    • Author(s)
      Muraoka D, Harada N, Hayashi T, Sawada S, Akiyoshi K, Shiku H.
    • Organizer
      the AACR Special Conference, Tumor Immunology : Multidisciplinary Science Driving Basic and Clinical Advances
    • Place of Presentation
      マイアミ,米国
    • Related Report
      2013 Final Research Report
  • [Presentation] ワクチン抗原のCHPナノゲル複合体化によるリンパ節輸送の促進と抗腫瘍免疫応答の改善2012

    • Author(s)
      吉見公志,村岡大輔,原田直純,林妙,秋吉一成,珠玖洋
    • Organizer
      日本癌学会
    • Place of Presentation
      ホテルロイトン札幌,札幌.
    • Related Report
      2013 Final Research Report
  • [Presentation] ワクチン抗原のCHPナノゲル複合体化によるリンパ節輸送の促進と抗腫瘍免疫応答の改善2012

    • Author(s)
      村岡大輔,原田直純,林妙,吉見公志,澤田晋一,秋吉一成,珠玖洋
    • Organizer
      日本がん免疫学会
    • Place of Presentation
      北海道大学,札幌.
    • Related Report
      2013 Final Research Report
  • [Presentation] Long Peptides Complexed with A Novel Delivery System CHP Nanogel Leads to the Improved Vaccine-induced Specific Immune Responses with CpG oligo DNA or poly-I2012

    • Author(s)
      Harada N, Muraoka D, Hayashi T, Yoshimi K, Sawada S, Akiyoshi K, Shiku H.
    • Organizer
      C RNAThe Association for Cancer Immunotherapy (CIMT) 2012 Annual Meeting
    • Place of Presentation
      Rheingoldhalle Congress Center, Mainz, Germany.
    • Related Report
      2013 Final Research Report
  • [Presentation] Long Peptides Complexed with A Novel Delivery Sytem CHP Nanogel Leads to the Improved Vaccine-induced Specific Immune2012

    • Author(s)
      Naozumi Harada, Daisuke Muraoka, Tae Hayashi, Koji Yoshimi, Shin-ichi Sawada, Kazunari Akiyoshi and Hiroshi Shiku
    • Organizer
      CIMT
    • Place of Presentation
      ドイツ
    • Related Report
      2012 Research-status Report
  • [Presentation] ワクチン抗原のCHPナノゲル複合体化によるリンパ節輸送の促進と抗腫瘍免疫応答の改善2012

    • Author(s)
      村岡 大輔、原田 直純、林 妙、吉見 公志、澤田 晋一 、秋吉 一成、珠玖 洋
    • Organizer
      日本がん免疫学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] ワクチン抗原のCHPナノゲル複合体化によるリンパ節輸送の促進と抗腫瘍免疫応答の改善2012

    • Author(s)
      吉見 公志、 村岡 大輔、 原田 直純、 林 妙、 秋吉 一成、 珠玖 洋
    • Organizer
      日本癌学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Book] バイオ医薬品2013

    • Author(s)
      原田直純、珠玖洋
    • Publisher
      化学同人
    • Related Report
      2012 Research-status Report
  • [Remarks] JSTイノベーション・ジャパン、JST東海国立3大学新技術説明会、BIOtech 2013、BioJapan 2013に出展

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] がん治療用ワクチン製剤2012

    • Inventor(s)
      原田直純,村岡大輔,秋吉一成,珠玖 洋
    • Industrial Property Rights Holder
      三重大学・東京医科歯科大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-08-30
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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