Cell Differentiation by DNA Quaternary Complex to Nucleus Event
Project/Area Number |
24650281
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 遺伝子複合体 / 細胞分化誘導 / アルキルイミダゾール末端修飾PEG / DNA一分子修飾 / アルキルイミダゾール末端修飾PEG / DNA一分子修飾 |
Research Abstract |
The new concept of DNA "mono-ion complex" had been established from the DNA modification method by the interaction of the alkyl group with the DNA groove. The resulting mono-ion complex has exhibited significant higher transfection activity for tibialis muscle than clinical naked DNA and commercial in vivo transfection reagent jet PEI. The achievement of transfection to mouse tibialis muscle cells with less mitosis suggests the possibility of exogenous gene transfection preserving their nucleus membrane. Therefore, the resulting tool to control nucleus event has been established for realizing the differentiation of cell with no mitosis.
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Report
(3 results)
Research Products
(33 results)
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[Patent(Industrial Property Rights)] 界面活性剤様化合物2013
Inventor(s)
朝山章一郎、野原敦、川上浩良、根岸洋一
Industrial Property Rights Holder
朝山章一郎、野原敦、川上浩良、根岸洋一
Industrial Property Rights Type
特許
Industrial Property Number
2013-056436
Filing Date
2013-03-19
Related Report
Overseas