Physiological functions of free branched-chain amino acids in nerve and brain

• Project/Area Number: 24650406
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Nagoya University
• Principal Investigator: SHIMOMURA Yoshiharu 名古屋大学, 生命農学研究科, 教授 (30162738)
• Co-Investigator(Kenkyū-buntansha): KITAURA Yasuyuki 名古屋大学, 大学院生命農学研究科, 助教 (90442954)
• Co-Investigator(Renkei-kenkyūsha): KADOTA Yoshihiro 名古屋大学, 大学院生命農学研究科, 特別研究員 (10724776)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分岐鎖アミノ酸 / 分岐鎖α-ケト酸脱水素酵素キナーゼ / コンディショナルノックアウト / マウス / 筋 / 神経 / 分岐鎖アミノ酸分解系 / コンディショナルノックアウトマウス / 脳・神経

Research Abstract

Branched-chain amino acid metabolism is suppressed by branched-chain alpha-ketoacid dehydrogenase kinase (BDK). In the present study, we have prepared conditional BDK-knockout (KO) mice to clarify the physiological functions of BCAAs. We first produced BDK-floxed mice for preparation of conditional BDK-KO mice. Using the mice, we prepared global (whole body) BDK-KO mice, and then muscle-specific BDK-KO mice, and now preparation of nerve-specific BDK-KO mice is ongoing. The conditional BDK-KO mice have a tissue-specific deficiency of BCAAs. Specific features of these mice are becoming to be clarified.

Research Products

• [Presentation] Characterization of muscle- specific branched-chain a-ketoacid dehydrogenase kinase (BDK)- deficient mice (2014)
  • Author(s): Ishikawa, T., Kitaura, Y., Itami, Y., Kadota, Y., Kato, R., Inukai, A., Shimomura, Y
  • Organizer: Experimental Biology 2014
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-28
• [Presentation] 筋特異的分岐鎖アミノ酸代謝亢進マウスの解析 (2014)
  • Author(s): 北浦靖之, 石川卓弥, 伊丹雄也, 加藤里奈, 犬飼彩美, 門田吉弘, 下村吉治
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 川崎市
  • Year and Date: 2014-03-29
• [Presentation] 筋特異的分岐鎖アミノ酸代謝亢進マウスの解析
  • Author(s): 北浦靖之、石川卓弥、伊丹雄也、加藤里奈、犬飼彩美、門田吉弘、下村吉治
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学（川崎市）