Search for new target molecules to overcome drug resistance using the Hsp72 binding protein interactome
| Project/Area Number |
24650647
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical oncology
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| Research Institution | Osaka City University |
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Principal Investigator |
TAKAHASHI KATSUYUKI 大阪市立大学, 医学(系)研究科(研究院), 薬剤部職員 (10597751)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 抗癌剤耐性 / 分子シャペロン / 熱ショックタンパク質 / プロテオミクス / シャペロン |
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| Research Abstract |
Heat shock protein 72 (Hsp72) has functions such as promotion of protein folding, stabilizing oncogenic proteins, and avoiding stress. It has reported that Hsp72 overexpressed in gastric cancer was associated with resistance to chemotherapy. We think that Hsp72 client proteins would play an important role in drug resistance.
All experiments used human gastric cancer cell line, 2M and oxaliplatin (OXA) resistant 2M (2M/OXA). We identified 22 specific Hsp72-client proteins in 2M/OXA by LC/MS/MS. One of the client proteins, stromal cell derived factor 2 siRNA enhanced OXA inducing cell death in 2M/OXA.
Hsp72-client proteins in drug-resistant cells may target molecules to overcome drug resistance.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Hsc70 contributes to cancer cell survival by preventing Rab1A degradation under stress conditions2014
Author(s)
Tanaka M, Mun S, Harada A, Ohkawa Y, Inagaki A, Sano S, Takahashi K, Izumi Y, Osada-Oka M, Wanibuchi H, Yamagata M, Yukimura T, Miura K, Shiota M, Iwao H
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Journal Title
PLOS ONE
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Fibroblast growth factor-2 anchoring on plasma membrane is critical for endothelial cell survival2014
Author(s)
Tanaka M, Yamaguchi M, Kawamoto Y, Takahashi K, Inagaki A, Osada-Oka M, Harada A, Wanibuchi H, Izumi Y, Miura K, Shiota M, Iwao H, Ohkawa Y
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Journal Title
Monoclon Antib Immunodiagn Immunother
Volume: (in press)
Related Report
Peer Reviewed
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[Journal Article] Fibroblast growth factor-2 anchoring on plasma membrane is critical for endothelial cell survival2014
Author(s)
Tanaka M, Yamaguchi M, Kawamoto Y, Takahashi K, Inagaki A, Osada-Oka M, Harada A, Wanibuchi H, Izumi Y, Miura K, Shiota M, Iwao H, Ohkawa Y.
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Journal Title
Monoclon Antib Immunodiagn Immunother
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Hsc70 contributes to cancer cell survival by preventing Rab1A degradation under stress conditions2014
Author(s)
Tanaka M, Mun S, Harada A, Ohkawa Y, Inagaki A, Sano S, Takahashi K, Izumi Y, Osada-Oka M, Wanibuchi H, Yamagata M, Yukimura T, Miura K, Shiota M, Iwao H.
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Journal Title
PLOS ONE
Volume: in press
Related Report
Peer Reviewed
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