Construction of Detection System for Ligand Screening in Solution Using Peptide Generation based on Molecular Interactions
| Project/Area Number |
24651237
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Gunma University |
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Principal Investigator |
TAKAHASHI Tsuyoshi 群馬大学, 先端科学研究指導者育成ユニット, 准教授 (90345380)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ペプチド / リガンド探索 / 分子間相互作用 / ペプチドライゲーション |
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| Research Abstract |
In order to develop a screening system in solution for ligands capable of binding to proteins, we have attempted to construct a ligation reaction between peptides bearing a ligand and a protein when the ligand-protein interaction occurs. Moreover, using the generated peptide, enzymatic activity can be generated. Using a model interaction pair, coiled-coil peptides, and ribonuclease S reconstitution, we have successfully demonstrated that the detection system can work well.
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Report
(3 results)
Research Products
(12 results)
