Study on biosynthesis and secretion mechanisms of the sperm attracting factor in ascidians

• Project/Area Number: 24651240
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: YOSHIDA Manabu 東京大学, 理学(系)研究科(研究院), 准教授 (60301785)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Kaoru 桐蔭横浜大学, 先端医用工学センター, 講師 (70398973)
• Co-Investigator(Renkei-kenkyūsha): MURATA Michio 大阪大学, 大学院理学研究科, 教授 (40183652)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 受精 / 生体分子 / 生理活性物質 / シグナル伝達 / 生体生命情報学 / RNA-Seq / 生理活性

Outline of Final Research Achievements

Sperm attraction of the egg found in many animals is believed to be a species-specific phenomenon in many cases. However, biosynthesis and secretion pathway of the sperm attractant in eggs is almost unknown. In this study, we tried to understand the universality and biodiversity of the sperm attraction system. First, in order to elucidate biosynthesis pathway and secretion of the sperm attractant SAAF, we have tried to comprehensive analysis of gene expression during oogenesis by RNA-seq analysis. Second, we have tried to identify sperm attractant from the ascidians related to Ciona intestinalis, and finally we identified the sperm attractant from the ascidian Ascidia sydneiensis.

Research Products

• [Journal Article] Chemotactic response with a constant delay-time mechanism in Ciona spermatozoa revealed by a high time resolution analysis of flagellar motility. (2015)
  • Author(s): Miyashiro, D. Shiba, K., Miyashita, T., Baba, S., Yoshida, M., and Kamimura, S.
  • Journal Title: Biology Open Volume: 4 Issue: 2 Pages: 109-118
  • DOI: 10.1242/bio.20137351
  • Peer Reviewed / Open Access
• [Journal Article] Sperm motility parameters and spermatozoa morphometric characterization in marine species: a study of swimmer and sessile species. (2014)
  • Author(s): Gallego, V., Perez, L., Asturiano, J. F., and Yoshida, M.
  • Journal Title: Theriogenology Volume: 82 Issue: 5 Pages: 668-676
  • DOI: 10.1016/j.theriogenology.2014.05.026
  • Peer Reviewed
• [Journal Article] Species-specificity of sperm motility activation and chemotaxis: a study on ascidian species. (2013)
  • Author(s): Yoshida, M., Hiradate, Y, Sensui, N., Cosson, J., and Morisawa, M.
  • Journal Title: Biological Bulletin Volume: 224 Pages: 156-165
  • Peer Reviewed
• [Journal Article] A Novel Sperm-Activating and Attracting Factor (SAAF) from the Ascidian Ascidia sydneiensis. (2013)
  • Author(s): Matsumori, N., Hiradate, Y., Shibata, H., Oishi, T., Simma, S., Toyoda, M., Hayashi, F., Yoshida, M, Murata, M, and Morisawa, M.
  • Journal Title: Organic Letters Volume: 15 Issue: 2 Pages: 294-297
  • DOI: 10.1021/ol303172n
  • Peer Reviewed
• [Journal Article] Role of Mos/MEK/ERK cascade and Cdk1 in Ca2+ oscillations in fertilized ascidian eggs. (2012)
  • Author(s): Sensui, Noburu
  • Journal Title: Developmental Biology Volume: 367 Issue: 2 Pages: 208-215
  • DOI: 10.1016/j.ydbio.2012.05.011
  • Peer Reviewed
• [Presentation] スジキレボヤから単離された精子活性化誘引物質の構造決定と化学合成 (2014)
  • Author(s): 渡部友博、海老根真琴、柴田 一、土川博史、松森信明、村田道雄、吉田 学、森沢正昭、大石 徹
  • Organizer: 第56回天然有機化合物討論会
  • Place of Presentation: 高知県立県民文化ホール他（高知県高知市）
  • Year and Date: 2014-10-15 – 2014-10-17
• [Presentation] 発現系を用いたCatSperチャネルの機能解析 (2014)
  • Author(s): 有馬大貴、筒井秀和、吉田 学、岡村康司
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 精子走化性における膜型カルシウムATPアーゼの役割 (2014)
  • Author(s): 吉田 薫、坂本恵香、稲葉一男、吉田 学
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] SAAF誘導体の活性比較によるSAAFの作用メカニズムの考察 (2014)
  • Author(s): 山本拓也, 小坂浩司, 鳥飼浩平, 吉田 薫, 大石 徹, 吉田 学
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Sperm chemotaxis is mediated by calcium extrusion via plasma membrane Ca2+-ATPase and Na+/Ca2+ exchanger. (2014)
  • Author(s): Yoshida, K, Shiba, K, Nakashima, A, Sakamoto, A, Matsunaga, S, Inaba, K, and Yoshida, M.
  • Organizer: The 12th International Symposium on Spermatology
  • Place of Presentation: Newcastle City Hall, Newcastle, NSW, Australia
  • Year and Date: 2014-08-08 – 2014-08-14
• [Presentation] 受精時における卵及び精子のカルシウムイメージング (2013)
  • Author(s): 吉田 学
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Invited
• [Presentation] Plasma membrane Ca2+/ATPase concerning sperm chemotaxis in the ascidian Ciona intestinalis (2013)
  • Author(s): 吉田薫、坂本恵香、稲葉一男、吉田 学
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] Species-specificity of sperm motility activation and chemotaxis: a study on ascidian species (2013)
  • Author(s): Yoshida M, Matsumori N, Hiradate Y, Sensui N, Cosson J, Shibata H, Oishi T, Murata M and Morisawa M
  • Organizer: Gordon Research Conference: Fertilization and the Activation of Development
  • Place of Presentation: Holderness School, Plymouth NH, USA
• [Presentation] CatSper mediates sperm penetration into the vitelline membrane in the Ascidian Ciona intestinalis (2013)
  • Author(s): Ono C, Maruyama K, Sakamoto A, and Yoshida M
  • Organizer: Gordon Research Conference: Fertilization and the Activation of Development
  • Place of Presentation: Holderness School, Plymouth NH, USA
• [Presentation] カタユウレイボヤの受精における精子特異的チャネルCatSperの働き (2012)
  • Author(s): 小野千紘，丸山虎轍，吉田 学
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学豊中キャンパス（豊中市）
• [Presentation] pHによるホヤ卵からの精子誘引物質放出の制御 (2012)
  • Author(s): 泉水 奏，柴 小菊，馬場昭次，稲葉一男，吉田 学
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学豊中キャンパス（豊中市）
• [Presentation] Sperm chemotaxis in the ascidians. (2012)
  • Author(s): Yoshida, M.
  • Organizer: The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Hotel Nagoya Garden Palace, Aichi, Japan
  • Invited
• [Presentation] Role for CatSper, Sperm-Specific Ca2+ Channel in Fertilization of the Ascidian Ciona intestinalis. (2012)
  • Author(s): Ono, C., Maruyama, K., Sakamoto, A., and Yoshida, M.
  • Organizer: The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Hotel Nagoya Garden Palace, Aichi, Japan
• [Presentation] カタユウレイボヤ精子誘引物質SAAFの卵からの放出にかかわる分子の解析
  • Author(s): 坂本恵香、吉田 薫、吉田 学
  • Organizer: 日本動物学会第65回関東支部会
  • Place of Presentation: 東京工業大学大岡山キャンパス（東京都目黒区）
• [Book] 動植物の受精学（第17章 精子運動の活性化と走化性） (2014)
  • Author(s): 澤田 均、稲葉一男、柴 小菊、吉田 学 他
  • Total Pages: 340
  • Publisher: 化学同人
• [Book] Sexual Reproduction in Animals and Plants (2014)
  • Author(s): Sawada H., Inoue N, Iwano M, Yoshida M, Hirohashi N, Matusaki M, Sasanami T, Burnett L, Chandler DE, et al.
  • Total Pages: 480
  • Publisher: Springer
• [Remarks] 東京大学三崎臨海実験所吉田グループホームページ
  • URL: http://www.mmbs.s.u-tokyo.ac.jp/research/Yoshida/index.html
• [Remarks] 東京大学大学院理学系研究科附属臨海実験所
  • URL: http://www.mmbs.s.u-tokyo.ac.jp/index.html
• [Remarks] 臨海実験所吉田グループFaceBookページ
  • URL: http://www.facebook.com/MMBS.YoshidaLab
• [Remarks] プレスリリース「ホヤ精子走化性の種特異性をもたらす精子誘引物質の構造の違いを解明」
  • URL: http://www.s.u-tokyo.ac.jp/ja/press/2013/02.html
• [Remarks] 東京大学大学院理学系研究科附属臨海実験所
  • URL: http://www.mmbs.s.u-tokyo.ac.jp/index.html
• [Remarks] 臨海実験所吉田グループFaceBookページ
  • URL: http://www.facebook.com/MMBS.YoshidaLab