Encapsulation mechanism of virus into protein crystals by in vivo crystallization
| Project/Area Number |
24651241
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
ABE SATOSHI 東京工業大学, 生命理工学研究科, 助教 (40508595)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 蛋白質結晶 / 細胞内結晶 / 多角体 / 昆虫ウィルス / 蛋白質結晶工学 |
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| Research Abstract |
Cypovirus produce the polyhedrin, then when the crystalline assemblies of polyhedrin are formed in cell, the virus particles are incorporated into the crystals. The virus particles embedded in polyhedra survive dehydration, freezing and enzymatic degradation for several years. However, the mechanism of polyhedrin crystallization and virus encapsulation is poorly understood. In this research, we demonstrated the mechanistic study of crystallization mechanism of polyhedra by observation of infectious cells with TEM and in vitro accumulation of polyhedrin with AFM. The results suggest that polyhedrin accumulation is closely affected with pH and potassium concentration in cell.
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Report
(3 results)
Research Products
(23 results)
