Development of fluorogenic probe for gene-analisis utilizing novel sililated fluorescent material
| Project/Area Number |
24651255
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | バイオプローブ / 含ケイ素高輝度蛍光剤 / 蛍光核酸プローブ / シリル化ピレン / 高輝度蛍光剤 |
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| Research Abstract |
Novel pyrene derivatives having modifiable silylated functional group were developed. Among these, a derivative possessing azide function was successfully incorporated into a modified DNA having alkynyl function via click chemistry. Also, a silylated pyrene possessing strong electron-withdrawing group (cyano function) was synthesized first time. The derivative exhibited marked red-shift on both absorption and emission spectra compare to that of the unmodified pyrene. In addition, fluorescent quantum yield of the derivative was increased almost twice as much as that of the unmodified pyrene. This unique derivative was further derived to the corresponding phosphoramide derivative and was incorporated into 5'-terminus of oligoDNA.
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Report
(3 results)
Research Products
(9 results)
