Dynamics elucidation of zooxanthellae in scleractinian coral by comprehensive genetic analysis

Research Project

Project/Area Number	24651267
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Resource conservation science
Research Institution	Kochi University
Principal Investigator	KUBOTA SATOSHI 高知大学, 教育研究部総合科学系, 准教授 (00314980)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	褐虫藻 / クレード / 網羅的解析 / 次世代シーケンサー / 造礁サンゴ
Outline of Final Research Achievements	The purpose of this study is to know the availability of next generation sequencer (NGS) for elucidation of zooxanthellae composition in scleractinian coral. Ribosomal RNA sequences of 28S region (LSU) was amplified and RFLP analysis and NGS analysis were applied. RFLP analysis showed several types of clade C and one clade F zooxanthellae in coral DNA. NGS analysis indicated major Clade C zooxanthellae species had identical sequences with analyzed by RFLP. This result supports that NGS must be powerful tool for elucidation of zooxanthella composition in scleractinian coral.