Dynamics elucidation of zooxanthellae in scleractinian coral by comprehensive genetic analysis

• Project/Area Number: 24651267
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Resource conservation science
• Research Institution: Kochi University
• Principal Investigator: KUBOTA SATOSHI 高知大学, 教育研究部総合科学系, 准教授 (00314980)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 褐虫藻 / クレード / 網羅的解析 / 次世代シーケンサー / 造礁サンゴ

Outline of Final Research Achievements

The purpose of this study is to know the availability of next generation sequencer (NGS) for elucidation of zooxanthellae composition in scleractinian coral. Ribosomal RNA sequences of 28S region (LSU) was amplified and RFLP analysis and NGS analysis were applied. RFLP analysis showed several types of clade C and one clade F zooxanthellae in coral DNA. NGS analysis indicated major Clade C zooxanthellae species had identical sequences with analyzed by RFLP. This result supports that NGS must be powerful tool for elucidation of zooxanthella composition in scleractinian coral.

Research Products

• [Journal Article] Molecular cytogenetic analysis of the scleractinian coral Acropora solitaryensis Veron & Wallace 1984. (2014)
  • Author(s): Taguchi T1, Mezaki T, Iwase F, Sekida S, Kubota S, Fukami H, Okuda K, Shinbo T, Oshima S, Iiguni Y, Testa JR, Tominaga A.
  • Journal Title: Zoolog Sci. Volume: 31 Issue: 2 Pages: 89-94
  • DOI: 10.2108/zsj.31.89
  • NAID: 40019989087
  • Peer Reviewed
• [Presentation] 次世代シーケンサーを用いた造礁サンゴに共生する褐虫藻の網羅的解析の試み (2012)
  • Author(s): 久保田 賢・目﨑 拓真
  • Organizer: 日本サンゴ礁学会
  • Place of Presentation: 東京大学