Development of a global gene identification method

• Project/Area Number: 24655155
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Okayama University
• Principal Investigator: SERA TAKASHI 岡山大学, 自然科学研究科, 教授 (10362443)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 遺伝子

Outline of Final Research Achievements

The goal of this study is to develop a method for a global gene identification. For this purpose, we designed nine molecules that recognized one specific target site of the gene of our interest, respectively, and generated them. Among them, we identified the best sample to modulate the expression of the target gene most effectively. Furthermore, we examined how to generate the phenotype of our purpose, and raised the induction efficiency to 60-70%.