| Project/Area Number |
24656314
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Prefectural University of Hiroshima |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 水素発酵 / メタン発酵 / リアルタイムPCR / mcrA gene / バイオガス化 / 微生物叢解析 / 機能遺伝子 |
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| Outline of Final Research Achievements |
In order to produce an efficient hydrogen-methane fermentation process, the behavior of microorganisms in a fermenter was examined by the activity of the gene.When hydrogen fermentation threw a raw material into a reactor, it was indicated that it's important to revise pH around 7.5.The hydrogen-methane fermented process needs one compared with the capacity of each reactor about 10 times.The determination method of mcrA gene created the primer set from the result of microflora or reference, and was examined by the real-time PCR method.The determination method of the amount of mcrA gene by the real-time PCR method has been established when the primer set of mcrA gene for Methanoculles obtained from reference was used.The expression level of mcrA gene in the continuous methane fermentation experiment system was quantified by real-time RT-PCR method which used the primer set of mcrA gene for Methanoculles.
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