Development of membrane protein reconstitution technique in vivo.
Project/Area Number |
24656506
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Okayama University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | タンパク質工学 / 化学修飾 / 細胞内導入 / タンパク質フォルディング / 細胞内輸送経路 / 翻訳系 / バイオテクノロジー / 膜タンパク質 / 細胞内輸送 / 細胞生物学 / タンパク質細胞内輸送 / 細胞機能制御 |
Research Abstract |
Combining protein transduction and in cell folding techniques, we tried to develop a novel in cell folding techniques for membrane protein in living cells. In order to achieve this technology, in cell folding technique was drastically improved in this study. Using denatured and reversibly cationized precursor protein, protein maturation events were observed in human cells without translation machinery.
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Report
(3 results)
Research Products
(21 results)