2013 Fiscal Year Final Research Report
Development of membrane protein reconstitution technique in vivo.
Project/Area Number |
24656506
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Okayama University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Keywords | タンパク質工学 / 化学修飾 / 細胞内導入 / タンパク質フォルディング / 細胞内輸送経路 / 翻訳系 |
Research Abstract |
Combining protein transduction and in cell folding techniques, we tried to develop a novel in cell folding techniques for membrane protein in living cells. In order to achieve this technology, in cell folding technique was drastically improved in this study. Using denatured and reversibly cationized precursor protein, protein maturation events were observed in human cells without translation machinery.
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Research Products
(12 results)