Real-time monitoring of protein levels
| Project/Area Number |
24657002
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUO Takuya 名古屋大学, 遺伝子実験施設, 助教 (00452201)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 生物時計 / リアルタイム測定 / 生物発光 / レポーター / ルシフェラーゼ / 変異体 / タンパク質 / 緑藻 / 時計タンパク質 / クラミドモナス |
|---|
| Outline of Final Research Achievements |
We tryed to develop a monitoring system for protein levels in vivo in real time and to apply the system to studies for the circadian clock. For this purpose, we used clock proteins of the green alga Chlamydomonas as a model. As a result, we succeeded in monitoring of levels in ROC15, ROC40, ROC66, and ROC75 Chlamydomonas clock proteins in vivo in real time. In addition, we succeeded in analyzing properties of the clock proteins by using this system.
|
Report
(4 results)
Research Products
(26 results)
-
[Journal Article] Importance of the monomer-dimer-tetramer interconversion of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria2015
Author(s)
Iida T, Mutoh, R, Onai, K, Morishita, M, Furukawa, Y, Namba, K, Ishiura, M
-
Journal Title
Genes Cells
Volume: 20
Issue: 3
Pages: 173-90
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Comparative analysis of kdp and ktr mutants reveals distinct roles of the potassium transporters in the model cyanobacterium Synechocystis sp. PCC 68032014
Author(s)
Nanatani, K., Shijuku, T., Takano, Y., Zulkifli, L., Yamazaki, Y., Tominaga, A., Souma, S., Onai, K., Morishita, M., Ishiura, M., Hagemann, M., Suzuki, I., Maruyama, H., Arai, F., Uozumi, N.
-
Journal Title
J. Bacteriology
Volume: -
Issue: 4
Pages: 676-87
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
[Journal Article] The promoter activities of sucrose phosphate synthase genes in rice, OsSPS1 and OsSPS11, are controlled by light and circadian clock, but not by sucrose.2013
Author(s)
Yonekura M, Aoki N, Hirose T, Onai K, Ishiura M, Okamur M, Ohsugi R, Ohto C
-
Journal Title
Frontiers Plant Sci.
Volume: 4
Pages: 31-31
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] The roles of the dimeric and tetrameric structures of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria2012
Author(s)
Murakami R, Mutoh R, Iwase R, Furukawa Y, Imada K, Onai K, Morishita M, Yasui S, Ishii K, Valencia Swain JO, Uzumaki T, Namba K, Ishiura M.
-
Journal Title
J Biol Chem.
Volume: 287
Issue: 35
Pages: 29506-29515
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
