| Project/Area Number |
24657004
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子間領域 / 非コードDNA / ゲノム / 遺伝学 / 大腸菌 / DARS / datA / oriC |
|---|
| Research Abstract |
In this study, we analyzed the inter-gene regions which have a length of more than 0.5 kb in the Escherichia coli genome. First, based on original analysis using informatics and E. coli genome database, we deduced 94 sites which meet our present study. Next, according to priority determined by further database analysis, we systematically constructed deletion mutants of the regions. One of those mutants was found to have clear sensitivity in cell growth at low temperatures. A specific sequence motif was also found in the region, which was suggested to support cell growth at low temperatures. In parallel, we analyzed the datA region which has a cluster of DnaA-binding sites in an inter-gene region. DnaA is the replication initiator protein and we revealed functional mechanism in datA for regulating DnaA,
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