Experimental demonstration of functional compensation among paralogous genes encoding neuropeptides
Project/Area Number |
24657050
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Animal physiology/Animal behavior
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Research Institution | The University of Tokyo |
Principal Investigator |
OKA Yoshitaka 東京大学, 理学(系)研究科(研究院), 教授 (70143360)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ペプチド / 生殖 / 性行動 / パラログ遺伝子 / トランスジェニック / メダカ / 神経科学 / 生理学 / 脳・神経 / 動物 / 生理活性 |
Research Abstract |
There is a general agreement that a whole genome duplication has occurred early during evolution to produce paralogous genes with diversified functions. Functional compensation of a deleted gene by its paralogous gene has been documented for unicellular organisms, but such functional compensation has not been demonstrated for multicellular organisms so far. We have recently found an example of possible functional compensation between the paralogous genes, kiss1 and kiss2, encoding peptides called kisspeptins, which are considered to be involved in the central regulation of reproduction and/or sexual behavior. Here, we attempted at demonstrating experimentally functional compensation mechanisms of kisspeptin genes using state-of-the-art gene knock-out techniques such as TALEN and CRISPR/Cas9, which can be applied to non-model organisms.
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Report
(3 results)
Research Products
(78 results)
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[Journal Article] A neural mechanism underlying mating preferences for familiar individuals in medaka fish.2014
Author(s)
Okuyama T, Yokoi S, Abe H, Isoe Y, Suehiro Y, Imada H, Tanaka M, Kawasaki T, Yuba S, Taniguchi Y, Kamei Y, Okubo K, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H
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Journal Title
Science (New York, N.Y.)
Volume: 343
Issue: 6166
Pages: 91-94
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Sexually dimorphic expression of the sex chromosome-linked genes cntfa and polim3a in the medaka brain.2014
Author(s)
Maehiro S, Takeuchi A, Yamashita J, Hiraki T, Kawabata Y, Nakasone K, Hosono K, Usami T, Paul-Prasanth B, Nagahama Y, Oka Y, Okubo K.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 455
Issue: 1
Pages: 113-119
DOI
Related Report
Peer Reviewed
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[Journal Article] Time-of-day dependent changes in GnRH1 neuronal activities and gonadotropin mRNA expression in a daily spawning fish, medaka2012
Author(s)
Karigo, T., Kanda, S., Abe, H., Okubo, K., and Oka, Y.
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Journal Title
Endocrinology
Volume: 153
Issue: 7
Pages: 3394-3404
DOI
Related Report
Peer Reviewed
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[Presentation] Morphological analysis of the early development of GnRH neuron systems in the EGFP-expressing transgenic medaka lines2013
Author(s)
Takahashi, A., Islam, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 43th Annual Meeting of the Society for Neuroscience 2013
Place of Presentation
San Diego
Related Report
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[Presentation] Morphological analysis of the early development of GnRH neuron systems in the EGFP-expressing transgenic medaka lines2013
Author(s)
Takahashi, A., Islam, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 43th Annual Meeting of the Society for Neuroscience 2013
Place of Presentation
San Diego,合衆国
Related Report
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[Presentation] Embryonic Expression and Function of Kisspeptins and their Receptors in a Teleost Fish, medaka2012
Author(s)
Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Weltzien, F.A.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo
Related Report
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[Presentation] Electrophysiological and Morphological Analysis of Kiss1 Neurons in Trasgenic Medaka, Oryzias latipes2012
Author(s)
Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo
Related Report
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[Presentation] Effects of Kisspeptins on LH Secretion and GnRH1 Neuronal Activities in Goldfish and Medaka2012
Author(s)
Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo
Related Report
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[Presentation] Embryonic Expression and Function of Kisspeptins and their Receptors in a Teleost Fish, medaka2012
Author(s)
Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Weltzien, F.A.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
The University of Tokyo, Tokyo
Related Report
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[Presentation] Electrophysiological and Morphological Analysis of Kiss1 Neurons in Trasgenic Medaka, Oryzias latipes2012
Author(s)
Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
The University of Tokyo, Tokyo
Related Report
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[Presentation] Effects of Kisspeptins on LH Secretion and GnRH1 Neuronal Activities in Goldfish and Medaka.2012
Author(s)
Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
The University of Tokyo, Tokyo
Related Report
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